Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F0 I0 R1 81 326.4 10500568 93.1% 9776028 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,724 G→T 19.9% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7240GT19.9% 199.6 / 6.9 96intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (35/37);  new base T (13/8);  total (49/47)
Fisher's exact test for biased strand distribution p-value = 3.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  CP009273/2170575‑2170873
                                                                                                                                                     |                                                                                                                                                        
tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATg                                                                                                                                                          >  2:1987495/1‑150 (MQ=255)
tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAATATAATg                                                                                                                                                          >  2:4616500/1‑150 (MQ=255)
  tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                                        <  1:4818774/150‑1 (MQ=255)
  tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTATATTTAAAAAAAAAATTaa                                                                                                                                                        >  1:2522353/1‑150 (MQ=255)
   cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                                       <  2:4463116/150‑1 (MQ=255)
    tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                                      <  1:4762431/150‑1 (MQ=255)
    tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                                      <  2:3694056/150‑1 (MQ=255)
     accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                                     <  1:3083802/150‑1 (MQ=255)
         ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                                 <  1:3927872/150‑1 (MQ=255)
          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTg                                                                                                                                                <  1:603958/150‑1 (MQ=255)
             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                                             <  1:4739655/150‑1 (MQ=255)
             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                                             <  1:4739741/150‑1 (MQ=255)
                 aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                                         <  2:22190/150‑1 (MQ=255)
                 aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAAATAAATTATtttttttttt                                                                                                                                         >  2:3504050/1‑148 (MQ=255)
                  tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaaa                                                                                                                                        <  1:3203460/150‑1 (MQ=255)
                   cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAat                                                                                                                                       <  1:974359/150‑1 (MQ=255)
                     tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                                     <  1:345687/150‑1 (MQ=255)
                       tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                   <  2:416786/150‑1 (MQ=255)
                       tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                                   <  2:1509140/150‑1 (MQ=255)
                         gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                                                 <  2:485418/150‑1 (MQ=255)
                           gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                               <  1:2771144/150‑1 (MQ=255)
                            aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATAAAATGAAAATATTTTTTTTTAAAAATCCCGATa                                                                                                                              >  2:3870585/1‑150 (MQ=255)
                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                                             <  2:4187871/150‑1 (MQ=255)
                              tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                                            <  2:1299867/150‑1 (MQ=255)
                                tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTAAAAATATAATGTAATTTTTTTTTTTTTAATATCTAGAttaaga                                                                                                                          >  2:3273150/1‑145 (MQ=255)
                                tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGTAATTATTTTTTTTTTAATATAGAGATAACGa                                                                                                                          >  2:214503/1‑150 (MQ=255)
                                tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAATTAATTTTTTTTAAAAATAGAGATAAcca                                                                                                                          >  2:307510/1‑148 (MQ=255)
                                tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGGTTTAGATTTAAAAATATAAAGAAAATATTTTTTTTTAAATATACAGATAAAGa                                                                                                                          >  2:5080309/1‑150 (MQ=255)
                                tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAAAGAAATTATTTTTTTTTTAATATAGGGTTAACGa                                                                                                                          >  2:213609/1‑150 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATTAAAATATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                         >  2:767628/1‑150 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGAt                                                                                                                         >  2:767951/1‑150 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAAAGAAATTATTTTTTTTTAAATATAGAGATAACGAt                                                                                                                         >  2:767565/1‑150 (MQ=255)
                                   atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTGTTTTGTTTTGAAAAAATAATTTAATTATTTTTTTTTTAATTTGTAGATTACgcata                                                                                                                       >  1:1012205/1‑146 (MQ=255)
                                   atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATAGGGATAAAGATca                                                                                                                       >  2:2566909/1‑150 (MQ=255)
                                   atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTTTGTTTGAATATATAATTTAAATATTTTTTTTTTTATTAAGAGATAAAGATca                                                                                                                       >  1:1012937/1‑150 (MQ=255)
                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                                      <  1:1108668/150‑1 (MQ=255)
                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                                      <  1:547573/150‑1 (MQ=255)
                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAGTGAAATTATTTGTTTTTAAATATCGAGATAACGATcac‑a                                                                                                                    <  2:377956/150‑1 (MQ=255)
                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac‑a                                                                                                                    <  2:377881/150‑1 (MQ=255)
                                               ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAAAAAAAAGAAAATATTTTTTTTTTAATATAGAGATTACGATAAC‑AAAAACGACac                                                                                                          >  2:2271383/1‑149 (MQ=255)
                                                     gaaTAACCGCCGCGCTGTGCGTTTTTCAGCATCTTCTTTGTCGATACCAGTTAAATTTTCATATCCTGTCTTTTGTTTTCTATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCAA‑AAAAACGACAATATGaa                                                                                                    <  2:4464957/148‑1 (MQ=255)
                                                      cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAAAATATAGATAAAGATAAA‑AAAAACGACAAAATTaaa                                                                                                   >  2:1693891/1‑150 (MQ=255)
                                                           ccgccgCGGTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGAAAATTTACATATCCTTTCGTTTTTTTTCGATTTAAAAATATAATTAAATTATTTTTTTTAAAATATCTAGATAACTATAAA‑AAAAACGACAATATGAAAattat                                                                                              <  1:3480247/150‑1 (MQ=255)
                                                               cgcgGTGTGCTTTGTTCAGAATCTGCTTTGTCGATACCACGTACATTTTAATATCCTGTCGTTTTTTTTCGTTTTAAAAATATATTTAAATTATTTTTTTTTAAATATCGAGATACCGATCAC‑AAAAACGACAATATGAAAATTATTCga                                                                                          <  2:2776971/150‑1 (MQ=255)
                                                               cgcgCTGTGCGTTGTTCAGAATCGGCTTTGTCGAAACCACGTACATTTACATATCCTGTCGTTTTTTTACTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCga                                                                                          <  2:2777110/150‑1 (MQ=255)
                                                                   cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATATAATTTAAATATTTTTTTTTAAATATAGAGGTTAAGATAAA‑AAAAAAGAAAAAATTAAAATTATTAgagggg                                                                                      >  1:2167970/1‑148 (MQ=255)
                                                                     gtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTGAAAAAAAAATTAAAATAATTTTTTTTAAATATAGAGATAAACAAAAC‑AAAAAAGAAAAAAATAAAAATATTAgaggaggg                                                                                    >  1:3057885/1‑148 (MQ=255)
                                                                     gtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAAAAAAGAAAATATTTTTTTTTAAATAACGATATAAACATCAC‑AAAAAAGACAAAATTAAAAATATTCGTGGtgtg                                                                                    >  1:3056662/1‑150 (MQ=255)
                                                                      tgCGTTGTTCAGCATCTGCTTTGTCTATACCACGTAAATTTTCATATCCTGTCTTTTTTTTTCTATTTCAAAATAAAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑a                                                                                  <  2:1266266/150‑1 (MQ=255)
                                                                         gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTTAATTATTTGTTTTTAAATATAGATATAAAGATCAA‑AAAAAAAACAAAATTAAAATTATTAGAGGAGTG‑AAA‑g                                                                              >  2:1934109/1‑150 (MQ=255)
                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGTTTTAAAAAAATAATTAAAATATTTTTTTTTTTATTTAGAGATTAAGATAAA‑AAAAAAGACAAAAAGAAAAATATTATAGGAGTT‑AAATTGCaaaaaaa                                                                     >  2:1021149/1‑150 (MQ=255)
                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAAAAAAAAAAAAATATTTTTTTTTAAAAAAAGAGATAAACATAAC‑AAAAAAAAAAAAAAGTAAAATATTAGATGAGTGTAAT‑GACaaaaaaaa                                                                    >  2:498811/1‑149 (MQ=255)
                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAAAAAAATTAAATTATTTTTTTTTAAAAATAGTGAAAAAGAAAAA‑AAAAAAAAAAAAATTTAAAATATTAGAGGATTG‑TAA‑GGGaaaaaaaaa                                                                   >  1:2103648/1‑148 (MQ=255)
                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTGTGTTTTTAAAAAAAAATGAAATTATTAGTTTTTATATATAGATATAAACAAGACAAAAAACAAAAAAATTAAAA‑TATACGAGTATTG‑AAA‑TCaaaaaaaaaa                                                                   >  1:2103640/1‑148 (MQ=255)
                                                                                      tgatttGTCGATACAACTTACATTTTAATATCCTTTCGTTTTTTTTCTATTTAAAAATATATTTAAATTATTTTTTTTTAAATATCTATATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGc                                                                 <  2:2522353/147‑1 (MQ=255)
                                                                                              cGATACCACTTATATTTTCTTATCATGTCGTTTTTTTTCTTTTTAAAAATATAATTAAATTATTTGTTTTTAATTATCGATATAACGATCAA‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGAt                                                         <  2:4467665/150‑1 (MQ=255)
                                                                                               gATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTCAAAAAATAATGAAAATATTTTTTTTTAAATATAGAGATAAAGAACAC‑AAAAACGACAAAATGAAAATTATTAGAGGCGTG‑TAA‑GGCAAAAAAAAGGCCTCCCGCTa                                                        >  2:5223672/1‑150 (MQ=255)
                                                                                               gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTTAAATATTTTTTTTTAAATATAGAGATAACGATAAC‑AAAAAACACAATATTAAAAATATTCGAGGAGTG‑AAA‑GGCCAAAAAAAGGCCACCCGATa                                                        >  2:5224471/1‑150 (MQ=255)
                                                                                               gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAAAAATGAAATTATTTTTTTTTTAATATAGAGATAAAGATAAC‑AAAAAAGAAAAAATTAAAATTATTAGAGGAGTG‑TAA‑TGCAAAAAAAACGCCAccccaga                                                        >  2:5224608/1‑146 (MQ=255)
                                                                                                aTACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGATATAAAGATCAC‑AAAAAAGAAAATATGAAAATTATTAGTGGATTG‑AAA‑GGCAAAAAAAAGGGCTCCCGCTat                                                       >  2:2010191/1‑149 (MQ=255)
                                                                                                aTACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAAAAAAATGAAATTATTTTTTTTTATATATCGAGATAAAGATAAC‑AAAAACGACAATATGAAAATTATTAGAGGTGTG‑AAA‑GGGCAAAAAACTGCCTACCGGTAg                                                       >  2:3495883/1‑150 (MQ=255)
                                                                                                 tACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTGAAAATATAATTTAATTATTTTTTTTTTTATATCGGGTTTAAGATAAC‑AAAAAACACAAAATGGGCAGTATTGGGGGGGTG‑GAT‑GGGAAAAAAAAGGCCGCCCgcgagg                                                      >  2:4057998/1‑145 (MQ=255)
                                                                                                 tACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAATGTAAATATTTTTTTTTTAATATCGAGATGACGATCAC‑AAAAAAGACAAAATGAAAAATATTAGAGGAGGG‑ACA‑TGCGAAAAAACGGCCTCCCGAGAgg                                                      >  2:4057948/1‑150 (MQ=255)
                                                                                                      cttaaaTTTTAATATCCTGTGTTTTTTTTTCTATTAAAAAATATATTGAAATTATTTTTTTTTAAATATCTATATACCGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGc                                                 <  1:2431808/145‑1 (MQ=255)
                                                                                                       ttaatttttCATATCCTGTCTTTTGTTTTATATTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCTATATAACTATAAA‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                                <  1:4616500/145‑1 (MQ=255)
                                                                                                       ttaatttttCATATCCTGTCGTTTTTTTTCTTTTTCAAAATATAATGAATTTATTTTTTTTTAAATTTCTAGATAACGATCAA‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                                <  1:1987495/145‑1 (MQ=255)
                                                                                                             tttCATATCCTGTCGTGTTTTTTAGATTTAAAAATATAAAGAAATTATTTTTTTTTAAAAATAGAGCTAACGATAAC‑AAAAAAGACAAAAATAAAAATATTAGAGGAGTG‑AAA‑GGCAAAAAAAAGGCCAACCCATATGGAAGCCGTAGCa                                          >  2:5166828/1‑150 (MQ=255)
                                                                                                             ttaaatatCCTGTCGTTTGTTTTCTATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACTATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCa                                          <  2:2979368/146‑1 (MQ=255)
                                                                                                                cATATCCTGTCGTTTGTTTTCTATTACAAAATATAATTAAATTATTTGTTTTTAAATATCTATATAACGAACAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAg                                       <  1:2554921/150‑1 (MQ=255)
                                                                                                                  tttCCTGTCGTTTTTTTTCGATTACAAAATAAAATGAAATTATTTGTTTTTAAATATCGATATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                                     <  2:1144225/148‑1 (MQ=255)
                                                                                                                   atCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATACCGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                                    <  1:2596408/150‑1 (MQ=255)
                                                                                                                          cGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                             <  2:2455835/150‑1 (MQ=255)
                                                                                                                             ttgtttTCGATTTCAAAATATATTTAAATTATTTTTTTTTAAATATCGATATAACGATCAA‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                          <  1:395322/150‑1 (MQ=255)
                                                                                                                             ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                          >  1:2959834/1‑150 (MQ=255)
                                                                                                                             ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                          >  1:3971814/1‑150 (MQ=255)
                                                                                                                              tgtttTCTATAACAAAATATATTCAATTTATTTTTTTTTAAATATCGATAAAACTAAAAA‑AAACACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                         <  1:4422524/150‑1 (MQ=255)
                                                                                                                               gtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                        >  1:3341732/1‑150 (MQ=255)
                                                                                                                                  ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  1:1847854/1‑150 (MQ=255)
                                                                                                                                  ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  1:1847877/1‑150 (MQ=255)
                                                                                                                                  ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATGCGAGGAGTG‑AAA‑GGCAAAATAACGGCCTCCCGATAGGGAAGCCGTAGCACAGTGCGCGTGTGGTTATGcc                     >  1:448815/1‑150 (MQ=255)
                                                                                                                                      aTTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGCGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                 <  2:4713653/150‑1 (MQ=255)
                                                                                                                                      aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAACACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                 <  1:2575872/150‑1 (MQ=255)
                                                                                                                                       tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg                >  1:4631949/1‑150 (MQ=255)
                                                                                                                                        ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc               >  1:811197/1‑150 (MQ=255)
                                                                                                                                         tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGTGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg              >  2:71171/1‑150 (MQ=255)
                                                                                                                                         tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGGTTTTATGCCGGATGCg              >  2:73531/1‑150 (MQ=255)
                                                                                                                                           aaaaTATAATGAAATTATTTGTTTTTAAATGTCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            >  1:339988/1‑150 (MQ=255)
                                                                                                                                           aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            >  2:4348510/1‑150 (MQ=255)
                                                                                                                                           aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            >  2:1036777/1‑150 (MQ=255)
                                                                                                                                            aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           <  2:4439827/150‑1 (MQ=255)
                                                                                                                                            aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           >  1:4716834/1‑150 (MQ=255)
                                                                                                                                               tataTTAAAATTATTTTTTTTTAAATATCGAGATAACGAGCAC‑AAACACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa        <  2:458584/150‑1 (MQ=255)
                                                                                                                                               tataATGAAATTATTTGTTTTTAAATATCGAGATAACGAACAC‑AAACACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa        <  2:459403/150‑1 (MQ=255)
                                                                                                                                               tataATGAAATTATTTGTTTTTAAATAGCGAGATAACGATCAC‑AAAACCGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa        <  2:5167729/150‑1 (MQ=255)
                                                                                                                                                ataATGAAATTATTTGTTTTTAAATATCGAGATACCGATCAC‑ACACACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa       <  2:4109959/150‑1 (MQ=255)
                                                                                                                                                ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa       >  1:3794438/1‑150 (MQ=255)
                                                                                                                                                 taATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc      >  1:16344/1‑150 (MQ=255)
                                                                                                                                                  aaTGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa     >  2:1974281/1‑150 (MQ=255)
                                                                                                                                                     gAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc  >  2:3019841/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                        
TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAA‑GGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  CP009273/2170575‑2170873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A