Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,724 G→T 21.7% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7240GT21.7% 499.9 / 29.1 251intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (90/93);  new base T (46/16);  total (137/114)
Fisher's exact test for biased strand distribution p-value = 6.41e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 254 total aligned reads displayed.

TTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAT  >  CP009273/2170576‑2170871
                                                                                                                                                    |                                                                                                                                                   
ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATg                                                                                                                                                     <  2:3607135/149‑1 (MQ=255)
  cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                                   <  2:1400569/149‑1 (MQ=255)
   tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                                  <  2:293900/149‑1 (MQ=255)
   tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                                  <  2:1905461/149‑1 (MQ=255)
   tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAAAGaaa                                                                                                                                                  >  2:109543/1‑149 (MQ=255)
      caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTGATTTAAAAAAATAATTAAaata                                                                                                                                               >  1:1043749/1‑149 (MQ=255)
        ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                             <  2:781887/149‑1 (MQ=255)
         cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAAATAttt                                                                                                                                            >  2:1250580/1‑149 (MQ=255)
         cacGTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                            <  2:1944183/149‑1 (MQ=255)
           cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                                          <  2:3719644/149‑1 (MQ=255)
           cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                                          <  2:1330623/149‑1 (MQ=255)
            ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAATTAAAAAAtttttt                                                                                                                                         >  1:783400/1‑149 (MQ=255)
              gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                                       <  1:1019995/149‑1 (MQ=255)
                aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                     >  2:4277941/1‑149 (MQ=255)
                aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAATGAAAATATTTGtttttt                                                                                                                                     >  2:2727391/1‑148 (MQ=255)
                aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                     <  1:3215766/149‑1 (MQ=255)
                aTCGTGTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                     <  2:4508264/149‑1 (MQ=255)
                 tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTTCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                                    <  2:4478331/149‑1 (MQ=255)
                 tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAATAAATTATttttttttta                                                                                                                                    >  2:2131244/1‑147 (MQ=255)
                   gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAAAAAAGAAATTATTTTTTTTTAAat                                                                                                                                  >  1:1990323/1‑149 (MQ=255)
                    tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAATTAAAATAATTTTTTTTaaaaa                                                                                                                                 >  2:4239872/1‑147 (MQ=255)
                    tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATAAAATTAAAATAATTTTTTTTAAata                                                                                                                                 >  1:182572/1‑149 (MQ=255)
                     ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                                <  1:4015571/149‑1 (MQ=255)
                     ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATAAAAAGAAATTATTTTTTTTTAAatat                                                                                                                                >  2:107800/1‑149 (MQ=255)
                      tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                               <  2:4270665/149‑1 (MQ=255)
                      tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                               <  1:1711840/149‑1 (MQ=255)
                      tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGAATTAAAAAAATAAATAAATTATTTTTTTTTTAaaata                                                                                                                               >  1:3536648/1‑148 (MQ=255)
                        gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                             <  2:3219211/149‑1 (MQ=255)
                         agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAATTAAATATTTTTTTTTAAATATagag                                                                                                                            >  2:4612606/1‑149 (MQ=255)
                          gtttGTGTATATTGAATGCCGGAACCGCATAACCTCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTAGATACCACGTACATTTTCAGATCCTGTCGTTTGTTTTCGATTTCAAAATATATTGAAATTATTTGTTTTTATATTTCgaga                                                                                                                           <  2:2419702/147‑1 (MQ=255)
                          gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgaga                                                                                                                           <  1:2499862/149‑1 (MQ=255)
                           aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                          <  1:535094/149‑1 (MQ=255)
                           aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                          <  1:1699256/149‑1 (MQ=255)
                             tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTGTTAGATTTAAAAAAATAATGAAATTATTTGTTTTTATATATCgagctca                                                                                                                        >  1:3729132/1‑145 (MQ=255)
                              gtgAATTTTGATTGCCGGAACCGCATATCCGCCGCGCTGTGAGTTGTTCAGGATCTGCTTTGTCGATACCACGTACTTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATTTCGAGATatc                                                                                                                       <  1:3673453/149‑3 (MQ=255)
                              gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGTTTTGAAAAAATAATTAAAATAATTTTTTTTTAATATAGATATTAc                                                                                                                       >  1:4472670/1‑149 (MQ=255)
                                gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                                     <  1:2242559/149‑1 (MQ=255)
                                 aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                    <  2:2497768/149‑1 (MQ=255)
                                 aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCAGATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                    <  2:2527849/149‑1 (MQ=255)
                                     ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAATATTATTAAATTATTTTTTTTTTAATATAGAGATAACGATaaca                                                                                                                >  2:561847/1‑149 (MQ=255)
                                         aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                            <  1:2944400/149‑1 (MQ=255)
                                          tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACAATCACAAAAAc                                                                                                           <  2:960173/149‑1 (MQ=255)
                                            ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                         <  2:1180037/149‑1 (MQ=255)
                                              ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTATATTTAAAAATATAAATTAAATATTTTTTTTTAAAAATAGAGATAAAGATCACaaaaaacaca                                                                                                       >  1:1145551/1‑144 (MQ=255)
                                              ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGTTTTTAAAATATAATTTAATTATTTTTTTTTTAATATATGGATAAAGATAACAAAAAAGACa                                                                                                       >  1:500061/1‑149 (MQ=255)
                                                aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTGAAAATATAATGTAAATATTTTTTTTTAAAAATAGTGATTAAGATAACCAAAACGACaaa                                                                                                     >  1:4285796/1‑148 (MQ=255)
                                                 acctaaTAACCGCCGCGGTGTGTTTTGTTCTGCTTGTGATTTGTCTATACCACTAACTTTTTAATTTCCTGTGTTTTTTTTTGGTTTTAAAAATATAATTAATTTTTTTTTTTTTAATTATGTATATAACGATCACAAAAACGACAata                                                                                                    <  2:4106002/144‑1 (MQ=255)
                                                 aCCGAATAACCGCCGCGGTGTGGTTTGTTCATCATCTGCTTTGTCTATACCACTTACTTTTTAATATCGTGTGGTTTTTTTTCTTTTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGATTTAACGAACACAAAAACGACAata                                                                                                    <  2:3367397/149‑1 (MQ=255)
                                                  ccGCATAACCGCCGCGCTGTGGGTTGTTCAGCATCTGATTTGTCTATACCACTTACTTTTTCATATCCTGTGGTTTGTTTTCTATTTCAAAATATAATGAATTTTTTTGTTTTTAATTTTCGAGATAACGATCAAAAACACGACAatat                                                                                                   <  2:92515/149‑1 (MQ=255)
                                                   cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATATTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATg                                                                                                  <  2:3170409/149‑1 (MQ=255)
                                                      aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGATTTAAAAATATAATTAAAATATTTTTTTTTTAATATAGAGGTAACGATAACAAAAAACACAAAATGaaa                                                                                               >  1:2644220/1‑149 (MQ=255)
                                                       tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCCCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaaa                                                                                              <  2:3569459/149‑1 (MQ=255)
                                                       tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAAAATCGAGATAACGAACAAAAAAAACACAAAAAGaaaa                                                                                              >  2:4100214/1‑149 (MQ=255)
                                                          ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAAAATAAATAAAATATTTTTTTTTAAATATAGAGATAACGATAACCAAAACGCCAATATgcacaata                                                                                           >  1:1540573/1‑142 (MQ=255)
                                                           cgccggGGTGTGGGTTGTTCAGCATCTGCTTTGTCGATCCCCCGTAAATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATTAATTTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattat                                                                                          <  1:2677480/149‑1 (MQ=255)
                                                            gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                         <  2:1143767/149‑1 (MQ=255)
                                                            gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAAATATTTTTTTTTAAATATAGAGATAACCATAACAAAAACCACAAAATGAAAaatatt                                                                                         >  2:1701763/1‑149 (MQ=255)
                                                                 gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGTTTTCTAAAAATAATGAAAATATTTTTTTTTTAAAATAGAGTTAAAGATAACAAAAAAACCAAAATTGAAATTATTCtagg                                                                                    >  2:805991/1‑149 (MQ=255)
                                                                     tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTGAAAATATAATTTAATTATTTTTTTTTTAATATAGATATAAAGATCACAAAAAAGACAAAATTGAAATTTTTAGAGGGGTg                                                                                >  1:2690813/1‑149 (MQ=255)
                                                                               aGCATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaa                                                                      <  2:2552036/149‑1 (MQ=255)
                                                                               aGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAAAATAATGTAAATATTTTTTTTTTAAAAAAGAGATAAAAATAACAAAAACAAAAAAATTAAAAATATTGTAGGTGTGGAAGGCcaaa                                                                      >  1:2270486/1‑149 (MQ=255)
                                                                                 cATCTGCTTTGTCGATACCACGTACATTTTCATATCCGGTCGTTTGTTTTCGTTTTAAAAATATATTGAATTTTTTTTTTTTTAATTATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaa                                                                    <  2:4526094/149‑1 (MQ=255)
                                                                                  aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGATTTGAAAAAAAAATTAAAAAATTTTTTTTTTAATATAGCGAAAACCATAACAAAAACAAAAAAAATAAAAAAATTAGAGGAGGGAAAAGCacaaaaa                                                                   >  1:4651389/1‑149 (MQ=255)
                                                                                        tttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTGTTTTAAAAATATAATTTAATTATTTTTTTTTTAATATAGATTTAAACATAAAAAAAAAGAAAATATTTAAATTATTATGGGAGTGGAATGCaaaaaaaaaggct                                                             >  2:2080768/1‑143 (MQ=255)
                                                                                          tGTCGAAACCACGTACATTTCAATATCCTGTCGTTTTTTTTCTATTTAAAAATATAATCAAATTATTTTTTTTTAAATATCTAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                           <  2:32808/149‑1 (MQ=255)
                                                                                             cGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAATGAAATTATTTTTTTTTTAATATAGAGAAAAAGATCAAAAAAAACACAAAATTAAAATTTTTAGAGGTGTGAAAGGCAAAAAAAAGGCCACCCGa                                                        >  2:1421696/1‑149 (MQ=255)
                                                                                              gATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAATATAAATTAAATATTTTTTTTTTAATATATAGATAAAGAAAAAAAAAAACAAAAAAATAAAAATATTAGTGGTTTGAAAGGCAAAAAAAAGGccaacccat                                                       >  1:1564670/1‑142 (MQ=255)
                                                                                                  ccACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATAACCAAAACGACAAAATTTAAATTATTGGAGGAGTGGTATGCCAAAAAAATGCCTCCCGCTAggg                                                   >  2:2999377/1‑149 (MQ=255)
                                                                                                   cACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAATATAAATAAATTATTTTTTTTTAAAAATAGAGATAACGATAACAAAAAAGACAAAATTAAAAATATTAGAGGAGTGTAAGGCAAAAAAAACGCCACCCGCTAgggc                                                  >  2:3787249/1‑148 (MQ=255)
                                                                                                      gTACATTTTCTTATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                               <  2:173982/149‑1 (MQ=255)
                                                                                                      gTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAAAATTATTCAATTATTTTTTTTTTAATATAGAGATTAACATCACACAAAAGCCAATATGAAAATTATTAGTGGTGGGTAAGGCCAAATATCGGCCTCCCGATAGGGCAGc                                               >  2:568202/1‑149 (MQ=255)
                                                                                                          tttttCATATCCTTTCTTTTGTTTTATATTAAAAAATAAAATCAATTTATTTTTTTTTAAATATCTATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                           <  1:3486001/148‑1 (MQ=255)
                                                                                                           ttttCATATCCTGTCGTTTGTTTTCTATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                          <  2:899098/149‑1 (MQ=255)
                                                                                                           ttttCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                          <  2:2648273/149‑1 (MQ=255)
                                                                                                             ttattatCCTGTCTTTTGTTTTCTTTTTAAAAATATAATGAATTTATTTGTTTTTAATTATCGAGATAACTAAAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCa                                        <  1:394420/145‑1 (MQ=255)
                                                                                                                        tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                             >  2:2991614/1‑149 (MQ=255)
                                                                                                                          gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                           >  2:1301119/1‑149 (MQ=255)
                                                                                                                          gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                           >  1:2299960/1‑149 (MQ=255)
                                                                                                                          gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                           >  1:2300068/1‑149 (MQ=255)
                                                                                                                            ttgtttTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                         <  2:3304059/149‑1 (MQ=255)
                                                                                                                              gtttTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                       <  2:656431/149‑1 (MQ=255)
                                                                                                                                 ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                    >  1:3188622/1‑149 (MQ=255)
                                                                                                                                   cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGACAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                  >  1:2937838/1‑149 (MQ=255)
                                                                                                                                   cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAATAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                  >  2:3394810/1‑149 (MQ=255)
                                                                                                                                   cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                  >  1:3821961/1‑149 (MQ=255)
                                                                                                                                    gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                 >  1:141117/1‑149 (MQ=255)
                                                                                                                                    gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                 >  1:3391221/1‑149 (MQ=255)
                                                                                                                                      tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGag               >  2:2763908/1‑148 (MQ=255)
                                                                                                                                        tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGCTAGGGAAGCCGTAGCCAAGTGCGCGTGGTTTTATGCCGGATGc             >  2:1647727/1‑149 (MQ=255)
                                                                                                                                        tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc             >  1:844285/1‑149 (MQ=255)
                                                                                                                                        acAAAATATATTTAAATTATTTTTTTTTAAATATCGATATAACTATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGTCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTCCGCGTGTTTTTATGCCGGATGc             <  1:3448672/148‑1 (MQ=255)
                                                                                                                                          aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg           >  2:4242969/1‑149 (MQ=255)
                                                                                                                                           aaaTATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt          <  2:3265487/149‑1 (MQ=255)
                                                                                                                                              tataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACACTATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGTTAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa       >  1:1437182/1‑149 (MQ=255)
                                                                                                                                               ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa      >  1:1887865/1‑149 (MQ=255)
                                                                                                                                                  aTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa   <  2:182572/149‑1 (MQ=255)
                                                                                                                                                  aTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa   >  2:2308819/1‑149 (MQ=255)
                                                                                                                                                   tGAATTTATTTGTTTTTAAATATCGATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt  <  1:109543/149‑1 (MQ=255)
                                                                                                                                                   tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt  >  1:2917864/1‑149 (MQ=255)
                                                                                                                                                   tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt  >  1:3522840/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAT  >  CP009273/2170576‑2170871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A