Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,724 G→T 23.7% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7240GT23.7% 330.7 / 27.5 174intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (51/71);  new base T (26/22);  total (78/96)
Fisher's exact test for biased strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  CP009273/2170575‑2170873
                                                                                                                                                      |                                                                                                                                                      
tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATg                                                                                                                                                        <  1:3315080/150‑1 (MQ=255)
         ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAT‑ATAATTAAATTAttt                                                                                                                                               >  2:3385771/1‑150 (MQ=255)
             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGttt                                                                                                                                           <  2:822304/150‑1 (MQ=255)
             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGttt                                                                                                                                           <  1:181709/150‑1 (MQ=255)
             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGttt                                                                                                                                           <  1:2445665/150‑1 (MQ=255)
              tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGtttt                                                                                                                                          <  1:388610/150‑1 (MQ=255)
               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTGATTTAAAAAT‑AAAAATAAAATAAtttttttt                                                                                                                                         >  1:3812385/1‑150 (MQ=255)
               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGttttt                                                                                                                                         <  2:3788234/150‑1 (MQ=255)
               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGttttt                                                                                                                                         <  2:1897204/150‑1 (MQ=255)
               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGttttt                                                                                                                                         <  2:1897199/150‑1 (MQ=255)
                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTa                                                                                                                                        <  1:2630114/150‑1 (MQ=255)
                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTa                                                                                                                                        <  1:1924147/150‑1 (MQ=255)
                   cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTATGTTTAAAAAT‑ATAATGAAATTATTTTTTTTTaata                                                                                                                                     >  1:580994/1‑148 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAata                                                                                                                                    <  2:70245/150‑1 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAata                                                                                                                                    <  1:3621866/150‑1 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAT‑ATAATGAAATTTTTTGTTTTTAAata                                                                                                                                    <  1:2580298/150‑1 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAT‑ATAATTAAATTATTTTTTTTTTAata                                                                                                                                    >  2:2968933/1‑150 (MQ=255)
                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATc                                                                                                                                  <  2:55250/150‑1 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAT‑ATAAAGAAAATATTTTTTTTTAAataaaga                                                                                                                                >  2:2205477/1‑146 (MQ=255)
                           gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTATATTTAAAAAA‑AAAAATAAAATTTTTTTTTTTTAATATAgagag                                                                                                                             >  1:4360840/1‑149 (MQ=255)
                              tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAAT‑ATAATGAAATTATTTTTTTTTTAATATCGCGAttaa                                                                                                                          >  2:4070953/1‑147 (MQ=255)
                               gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                                         <  1:1254511/150‑1 (MQ=255)
                                tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGAATTCAAAAT‑ATAATTAAAATATTTGTTTTTAAAAATAGAGATaaaca                                                                                                                        >  2:867191/1‑147 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                       <  1:248196/150‑1 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                                      <  1:4159715/150‑1 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTGAAAAT‑ATTATTATATTATTTTTTTTTTTATATAGAGTTAActtta                                                                                                                      >  1:2256525/1‑146 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTCGCTGTCAAAAA‑ATAAAGAAAATATTTTTTTTTAAAAATAGAGATAACCATc                                                                                                                      >  2:3273379/1‑150 (MQ=255)
                                   atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                                     <  2:3287789/150‑1 (MQ=255)
                                   atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAT‑ATAAAGAAAATATTTTTTTTTAAATATAGAGAAAAACATca                                                                                                                     >  2:372659/1‑150 (MQ=255)
                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                                   <  2:3284570/150‑1 (MQ=255)
                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                                   <  2:594734/150‑1 (MQ=255)
                                        gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                                                <  1:1357595/150‑1 (MQ=255)
                                         aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAAA‑ATAATGAAAATATTTTTTTTTTAATATAGAGTTAAAGATAACaaaaa                                                                                                               >  2:593552/1‑150 (MQ=255)
                                             ccGGAACCGCATAACCGCCGCGCTGTGCTTTGTTCAGCATCTGCTTTGACGATACCACGTACATTTTAATATCCTGTCGTTTGTTTTCTATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                                           <  2:3861171/150‑1 (MQ=255)
                                             ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                                           <  2:2073883/150‑1 (MQ=255)
                                              cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTGGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACa                                                                                                          <  2:1430404/150‑1 (MQ=255)
                                               ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACaa                                                                                                         <  2:1535003/150‑1 (MQ=255)
                                                  aCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGCTTTAAAAATAATAAT‑AAATTTTTTTTTTTTAAAAATCGAGATAAAGATCACAAAAACGACAatat                                                                                                      >  2:3107621/1‑150 (MQ=255)
                                                  aCCGAATAACCGCCGCGCTGTGCGTTGTACAGCATCTGATTTGTCGAAACAATGTAATTTTACATATCCTTTCGTTTTTTTTCTTTTTAAAAAT‑ATAATTAAATTTTTTTTTTTTAATTATCGAGATAACGAAAACAAAAACGACAatat                                                                                                      <  2:3016237/150‑1 (MQ=255)
                                                   ccGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAAA‑ATAAATAAATTATTTGTTTTTAAATATAGAGTTAACGATAACAAAAACGACAaaatt                                                                                                     >  2:4414685/1‑149 (MQ=255)
                                                       aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTCGATTTAAAAAT‑ATAAATAAATTATTTTTTTTTTAATATAGAGATAACGATCACAAAAACGACAATATGaaaa                                                                                                 >  2:648119/1‑150 (MQ=255)
                                                        tAACCGCGGGGGTGGGCGTTGTTCAGCATCTGCTTTGTCGATACAACTTACTTTTTAATATCGTGTGGTTTGTTTTCGATTTCAAAAT‑ATATTTAATTTTTTTTTTTTTAAATATCGTTATAACGATCACAAAAACGACAATATGAAAat                                                                                                <  1:718585/150‑1 (MQ=255)
                                                         aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGAATTCAAAAT‑ATAATTAAATTATTTTTTTTTTAATATCGAGATAACCATCAAAAAAACGACAAAATGAAAatt                                                                                               >  2:2583147/1‑150 (MQ=255)
                                                            cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTTAATATAGAGATAACGATAACAAAAAAGACAAAAAGAAAaatatt                                                                                            >  2:2130306/1‑150 (MQ=255)
                                                                gcgcTGTGTGTTGTTCAGCTTTTGTTTTGTCGAGACCACGTACTTTTTAATATCCTTTCTTTTGTTTTCTATTAAAAAAT‑ATAATCAAATTTTTTTTTTTTAAATATCGAGATAACTATCACAAACACGACAATATGAAAATTATTCgag                                                                                        <  1:2916070/150‑1 (MQ=255)
                                                                 cgcTGGGGGTTGTTCAGCATCTGCTTTGCCGATACCCCTAACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAAT‑ATAATTAAATTATTTGTTTTAAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagg                                                                                       <  1:1450883/150‑1 (MQ=255)
                                                                   cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgaggag                                                                                     <  2:1861334/150‑1 (MQ=255)
                                                                    tgtgCGTTGTTCAGAATCTGCTTTGTCGATCCCACGTAATTTTTCATATCGTGTCGTTTGTTTTCTATTACAAAAT‑ATATTGTAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGt                                                                                    <  2:4178842/150‑1 (MQ=255)
                                                                      tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGAATTAAAAAA‑AAAATTAAAATAATTTTTTTTTAAAAAAGAGATAAACATAACAAAAAAAACAAAAAGAAAAAAATTAGAGGCGtta                                                                                  >  2:3808012/1‑148 (MQ=255)
                                                                           tgtaCAGCATCTGCTTGGTCGATACCACGTAAATTTTCATATCCTGTCTTTTTTTTGCGATTTCAAAAT‑ATATTTAATTTTTTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGc                                                                            <  2:1916923/150‑1 (MQ=255)
                                                                            gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAT‑ATAAATAAATTATTTTTTTTTAAATATAGAGATAAAGATAACAAAAAACACAAAATTAAAATTATTAGAGGAGTG‑AAATGCa                                                                           >  2:206028/1‑150 (MQ=255)
                                                                                aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTGAAAAA‑ATAAATAAAATATTTTTTTTTTAATATAGAGATAAACAACACAAAAAAGAAAAAATTTAAAAAATTAGTGGAGTGTGAAGGCaaaa                                                                        >  2:2679043/1‑150 (MQ=255)
                                                                                aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTGAAAAT‑ATAATGAAAATATTTGTTTTTTAATATCGAGATTAAGATAACAAAAACCACAAAATGTAAATTATTCGTGGGGTG‑GAAGGCacaaa                                                                       >  2:3940629/1‑150 (MQ=255)
                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAA‑AAAATGAAATTAATTGTTTTTAAAAATAGAGATAAACATAACAAAAAAGACAAAATTAAAAATATTAGAGGAGTG‑AAAGGCaaaaaaaa                                                                    >  2:643728/1‑149 (MQ=255)
                                                                                    tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTCGATTTTAAAAT‑ATAATTAAAATATTTGTGTTTAAATATCGAGATAAAGAAAACACAAACACAAAAAATAAAATTATTCGAGGCTTG‑GAAGGCCACAAAAca                                                                   >  2:4208810/1‑149 (MQ=255)
                                                                                     cTGTTTTGTCGATCCCAATTAATTTTTAATATCTTTTCTTTTTTTTTCTATTAAAAAAT‑ATAATTAATTTTTTTTTTTTTAAATATCTATAAAACGAAAAAAAACACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACgg                                                                  <  1:867863/150‑1 (MQ=255)
                                                                                     cTGATTTTTCGATACCACTTAAATTTTCATATCCTGTCGTTTGTTTTCTATTACAAAAT‑ATAATGAATTTATTTTTTTTTAATTATCGATATAACGACCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACgg                                                                  <  2:1559435/150‑1 (MQ=255)
                                                                                              ctaTACCACTTACTTTTTCATATCCTTTCTTTTTTTTTCTATTTAAAAAT‑ATAATTAAATTTTTTTTTTTTAAATTTCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGAt                                                         <  2:1353530/148‑1 (MQ=255)
                                                                                              cGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAAT‑ATAATTAATTTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGAt                                                         <  2:1352204/150‑1 (MQ=255)
                                                                                              cGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGAt                                                         <  2:1352232/150‑1 (MQ=255)
                                                                                                aTACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTCAAAAT‑ATAAATAAAATATTTTTTTTTTTAAATAGAGATAAAGAGAACAAAAAACACCAAATGTAAATTAATAGAGGGGTG‑AAAGGCAAAAAAAAAGCCAcccccgag                                                       >  2:455974/1‑145 (MQ=255)
                                                                                                 aaCCACTTAAATTTTAATATCCTGTCTTTTTTTTTCTATTTAAAAAT‑ATATTGAAATTATTTTTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAgg                                                      <  1:3023716/149‑1 (MQ=255)
                                                                                                  acaaCGTAAATTTTCATATCCTGTCGTTTTTTTTGTATTTCAAAAT‑ATAATTAATTTATTTTTTTTTAAATATCTTTATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAggg                                                     <  1:2961047/147‑1 (MQ=255)
                                                                                                    cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                   <  2:1611546/150‑1 (MQ=255)
                                                                                                    cACGTAAATTTACATATCCTGTCGTTTGTTTTCGATTTAAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCTAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                   <  2:727696/150‑1 (MQ=255)
                                                                                                          atttttCATATCTTGTGTTTTTTTTTCTATTAAAAAAT‑ATAATTAAATTATTTTTTTTTAAATATCTATATAACGATCACAACCACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  1:973928/148‑1 (MQ=255)
                                                                                                          atttttCATACCATGTGTTTTTTTTTCTATTAAAAAAT‑ATAATTAATTTTTTTTTTTTTAAATTTCGATAAAAAGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  1:3238521/148‑1 (MQ=255)
                                                                                                               tCATATCCTGTCGTGTGTTTTGGATTTAAAAAA‑AAAAATAAAATATTTTTTTTTTAATAAAGACAAAAAGAACACAAAAAAGACAAAAATAAAATTATTAGAGGAGTG‑AAAGGCAAAAAAAAAGCCACCCCAAAAGGAAGCCCGAGCaaa                                        >  2:152040/1‑150 (MQ=255)
                                                                                                                  tctCCTTTTGTTTTTTTTCTATTACAAAAT‑ATAATTAAATTTTTTTTTTTTAAATATCGATAAACAGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                                     <  1:858406/148‑1 (MQ=255)
                                                                                                                  tatCCTGTGTTTTGTTTTCTATTAAAAAAT‑ATATTTAATTTTTTTTTTTTTAATTATCTATATAACTAAAAAAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                                     <  1:858526/150‑1 (MQ=255)
                                                                                                                        gtggtttttttTTGATTTCAAATT‑ATATTTAATTTTTTTGTTTTTAAATATCGTGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                               <  1:372659/147‑1 (MQ=255)
                                                                                                                        gTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                               >  2:3221570/1‑150 (MQ=255)
                                                                                                                          cGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                             >  2:3087557/1‑150 (MQ=255)
                                                                                                                           gtttgtttTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                            >  2:4018277/1‑150 (MQ=255)
                                                                                                                           gtttgtttTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                            >  2:1736046/1‑150 (MQ=255)
                                                                                                                              tgtttTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                         >  1:195279/1‑150 (MQ=255)
                                                                                                                              tgtttTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                         >  1:3135463/1‑150 (MQ=255)
                                                                                                                               gtttTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                        >  1:676068/1‑150 (MQ=255)
                                                                                                                               gtttTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                        >  1:676087/1‑150 (MQ=255)
                                                                                                                               gtttTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                        >  1:2409653/1‑150 (MQ=255)
                                                                                                                                ttttCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAATCGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                       >  1:4444992/1‑150 (MQ=255)
                                                                                                                                ttttCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                       >  1:3590242/1‑150 (MQ=255)
                                                                                                                                ttttCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                       >  1:2217388/1‑150 (MQ=255)
                                                                                                                                 tttCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                      >  1:766852/1‑150 (MQ=255)
                                                                                                                                    cGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                   >  1:506279/1‑150 (MQ=255)
                                                                                                                                     taTTTAAAAAT‑ATAATGAAATTTTTTTTTTTTAAATATCTAGATAACGAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                  <  1:1994752/149‑1 (MQ=255)
                                                                                                                                      aTTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                 >  1:4445950/1‑150 (MQ=255)
                                                                                                                                      aTTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                 >  1:3449752/1‑150 (MQ=255)
                                                                                                                                      aTTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTGTTTATGCCGGAt                 >  2:2359108/1‑150 (MQ=255)
                                                                                                                                          cAAAAT‑ATAATTAAATTATTTTTTTTTAATTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             <  2:3915226/150‑1 (MQ=255)
                                                                                                                                          cAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             >  1:3662138/1‑150 (MQ=255)
                                                                                                                                             aaT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt          >  2:271282/1‑150 (MQ=255)
                                                                                                                                             aaT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt          >  1:1835249/1‑150 (MQ=255)
                                                                                                                                             aaT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt          >  2:3884543/1‑150 (MQ=255)
                                                                                                                                              at‑atACTGAATTTATTTGTTTTTAAATATCGAGATCCCGATCACAACCACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa         <  1:2412494/150‑1 (MQ=255)
                                                                                                                                                    aTGAAATTATTTTTTTTTAAATATCTAGATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt    <  1:250223/150‑1 (MQ=255)
                                                                                                                                                    aTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt    >  1:2060646/1‑150 (MQ=255)
                                                                                                                                                    aTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt    >  2:784351/1‑150 (MQ=255)
                                                                                                                                                     tGAATTTATTTTTTTTTAAATATAGATAAAACTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa   <  1:1177777/150‑1 (MQ=255)
                                                                                                                                                      gAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc  <  2:3474078/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  CP009273/2170575‑2170873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A