Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F14 I0 R1 127 310.1 10402230 94.2% 9798900 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,724 G→T 24.7% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7240GT24.7% 528.5 / 37.3 251intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (79/98);  new base T (48/21);  total (130/121)
Fisher's exact test for biased strand distribution p-value = 6.00e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

TTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATA  >  CP009273/2170576‑2170872
                                                                                                                                                       |                                                                                                                                                     
ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATg                                                                                                                                                       <  2:888112/149‑1 (MQ=255)
ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATg                                                                                                                                                       <  2:1044139/149‑1 (MQ=255)
 tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGa                                                                                                                                                      <  2:4885191/149‑1 (MQ=255)
 tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGa                                                                                                                                                      <  2:4355707/149‑1 (MQ=255)
 tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGa                                                                                                                                                      <  2:3742332/149‑1 (MQ=255)
  cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGaa                                                                                                                                                     <  2:3323187/149‑1 (MQ=255)
   tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGaaa                                                                                                                                                    <  1:4354402/149‑1 (MQ=255)
    accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAat                                                                                                                                                   <  1:996134/149‑1 (MQ=255)
     ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTTG‑ATTTAAAAAA‑ATAATTAAat‑t                                                                                                                                                 >  2:1752002/1‑149 (MQ=255)
      caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAat‑ta                                                                                                                                                <  1:3664217/149‑1 (MQ=255)
      caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTAAAAAT‑ATAAATAAat‑ta                                                                                                                                                >  2:1590495/1‑149 (MQ=255)
       accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAat‑tat                                                                                                                                               <  1:2070442/149‑1 (MQ=255)
          acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGTAAT‑TATTTg                                                                                                                                            <  2:3058861/149‑1 (MQ=255)
          acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTg                                                                                                                                            <  2:4244081/149‑1 (MQ=255)
           cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAG‑ATTTAAAAAT‑AAAAATAAAA‑TAttttt                                                                                                                                           >  1:1106912/1‑147 (MQ=255)
            ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGtt                                                                                                                                          <  1:3427900/149‑1 (MQ=255)
            ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGtt                                                                                                                                          <  1:847957/149‑1 (MQ=255)
            ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGtt                                                                                                                                          <  1:4143533/149‑1 (MQ=255)
             tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGttt                                                                                                                                         <  1:2975494/149‑1 (MQ=255)
               cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCT‑ATTTAAAAAT‑ATAATTAAAA‑TATtttttttt                                                                                                                                       >  2:3589400/1‑149 (MQ=255)
               cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGttttt                                                                                                                                       <  1:803564/149‑1 (MQ=255)
                aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTGTTTT‑G‑ATTTAAAAAA‑ATAATTAAAA‑TATATTTTTTTa                                                                                                                                      >  1:4966693/1‑149 (MQ=255)
                aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAT‑AATTAAAAAT‑ATAATTAAAT‑TAAttttttttt                                                                                                                                      >  1:3355094/1‑148 (MQ=255)
                aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTa                                                                                                                                      <  1:1274660/149‑1 (MQ=255)
                 tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAG‑ATTTAAAAAT‑ATAAATAAAT‑TAATTTTTTTTaa                                                                                                                                     >  2:2947166/1‑149 (MQ=255)
                    tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAata                                                                                                                                  <  2:1084320/149‑1 (MQ=255)
                      tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATc                                                                                                                                <  1:687146/149‑1 (MQ=255)
                        gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCga                                                                                                                              <  2:2713705/149‑1 (MQ=255)
                         agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAG‑ATTTAAAAAA‑AAAAATAAAA‑TATTTTTTTTTTAATATAgaa                                                                                                                             >  2:1369561/1‑148 (MQ=255)
                          gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCgaga                                                                                                                            <  1:2640763/149‑1 (MQ=255)
                          gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAG‑AATTCAAAAA‑ATAATTAAAT‑TATTTGTTTTTAAATTTAgaga                                                                                                                            >  2:2061183/1‑149 (MQ=255)
                          gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTTGTTTTTAAAAAA‑AAAAATAAATATATTTTTTTTTatatataaa                                                                                                                              >  1:3062224/1‑146 (MQ=255)
                          gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAG‑ATTTAAAAAT‑ATAATGAAAA‑TATTTTTTTTTTAATATAgaga                                                                                                                            >  1:1201293/1‑149 (MQ=255)
                            ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATa                                                                                                                          <  2:3569042/149‑1 (MQ=255)
                            ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATa                                                                                                                          <  2:4155029/149‑1 (MQ=255)
                              gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAAc                                                                                                                        <  2:169658/149‑1 (MQ=255)
                               tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTGG‑ATTTAAAAAA‑ATAATGGAAA‑TATTTGTTTTTAAAAAACGAGATaaac                                                                                                                       >  2:4906035/1‑147 (MQ=255)
                                gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGTGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGa                                                                                                                      <  2:4681810/149‑1 (MQ=255)
                                 aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                     <  2:4076995/149‑1 (MQ=255)
                                  atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATc                                                                                                                    <  2:3623896/149‑1 (MQ=255)
                                  atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATc                                                                                                                    <  1:4515834/149‑1 (MQ=255)
                                  atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAT‑TTTTAAAAAA‑AAAAATAAAA‑AAATTTTTTTTTAATATATAGATAAAGaaa                                                                                                                    >  1:1444723/1‑147 (MQ=255)
                                    atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                                  <  1:4704468/149‑1 (MQ=255)
                                     ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAG‑ATTTAAAAAA‑ATAAATAAAA‑TATTTTTTTTTATATATCGAGATAAAGATaaca                                                                                                                 >  1:5186311/1‑149 (MQ=255)
                                              ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGGTTTAG‑ATTTCAAAAT‑ATAATGAAAA‑TATTTTTTTTTAAATATCGAGATAAAGATAACAAAAACGACa                                                                                                        >  2:3007551/1‑149 (MQ=255)
                                              ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGG‑ATTTAAAAAT‑ATAAATAAAA‑TAATTTTTTTTTAATATAGAGGTAAAGAAAACaaaaaaaaaa                                                                                                        >  1:2056954/1‑144 (MQ=255)
                                               gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCG‑ATTTGAAAAT‑ATAATGAAAT‑TATTTTTTTTTTAATATCGAGATTACGATCACAAAAAAGACaa                                                                                                       >  2:4877597/1‑149 (MQ=255)
                                                   cccATACCCGCCGCGCTGTGCGTTTTTCCGCATCTGCTTTGTCGATACCCCGTACATTTTCATATCCTGTCTT‑TTGTTTTCT‑ATTAAAAAAT‑ATAATGAATT‑TTTTTTTTTTTAAATATCTATATAACTATCACAAACACGACAATATg                                                                                                   <  2:3991136/147‑1 (MQ=255)
                                                       tAACCGCCGGGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCCCGTACATTTTAATATCCTGTCGT‑TTGTTTTCT‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaaa                                                                                               <  2:3368023/149‑1 (MQ=255)
                                                       tAACCGCCGCGCTGTGGTTTTTTCAGCATCTGTTTTGTCTATACCACGTACATTTTCATATCCTGTCTT‑TTGTTTTCG‑ATTTAAAAAT‑ATAATGAAAT‑TATTTTTTTTTAAATATCGAGATAACGATCAAAAAAACGACAATATGaaaa                                                                                               <  2:4536350/149‑1 (MQ=255)
                                                        atcagacgcgCTGTGCGTTGTTCACCATCTGCTTTGTCGATACCACTTACATTTAAATATCCTGTCGT‑TTGTTTTCT‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAat                                                                                              <  2:5160610/143‑1 (MQ=255)
                                                          ccgccgCGCTGTGCTTTGTACAGCATCTGCTTTGTCGATACCCCGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑TTTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAACACAAAAACGACAATATGAAAatta                                                                                            <  2:407553/149‑1 (MQ=255)
                                                          ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTAAAAAA‑ATAAAGAAAT‑TATTTTTTTTTAAATAACGAGATAACCAAAACAAAAACGACAATATGAAAatta                                                                                            >  2:2532941/1‑149 (MQ=255)
                                                            gccgcGCTGTGCGTTGTACAGCATCTGCTTTGTCGATACCACGTAAATTTTCATAACCTGTCGT‑TTTTTTTCG‑ATTACAAAAT‑ATAATGAAAT‑TATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAattatt                                                                                          <  2:5057987/149‑1 (MQ=255)
                                                                 gcggtgCGTTGTACAGCATCTGCTTTGTCTATACCACGTAAATTTTCATCGCCTGTCGT‑TTGTTTTCT‑TTTTCAAAAT‑ATATTGAAAT‑TATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCgagg                                                                                     <  1:2508782/146‑1 (MQ=255)
                                                                    gtgCGTTGTTCTGCATCTTCTTTTTCGATACCACGTAAATTTTAATATCCTGTCTT‑TTTTTTTCG‑ATTACAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGt                                                                                  <  2:617107/149‑1 (MQ=255)
                                                                        gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAG‑AATTAAAAAA‑ATAAAGAAAT‑TATTTTTTTTTAAATATAGAGATAACGATAAAAAAAACGAAAAAATTAAAATTATTAGAGGAGTGaaa                                                                              >  2:2857427/1‑149 (MQ=255)
                                                                        gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTTT‑TTTTAAAAATAAAAATAAAAT‑AATTTTTTTTTAAA‑AAATAGATAAAAAAAAAAAAAAAGACAATAATAAAATTATTAGAAGAGttgta                                                                              >  1:1583049/1‑145 (MQ=255)
                                                                          tttacAGAATCTGTTTTGTCTATACAAGGTAAATTTTCATCTCATTTCTT‑TTTTTTTCT‑ATTAAAAAAA‑ATAATTAAAT‑TATTTTTTTTTAAATATCTAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                            <  2:4460101/145‑1 (MQ=255)
                                                                               aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAG‑ATTTAAAAAA‑ATAAATCAAA‑TATTTTTTTTTTAAAAAAGGGATAACGATAACAAAAACCAAAAAATGTAAATTATTCGGGGGGGGAAAGGCacaa                                                                       >  2:1498465/1‑149 (MQ=255)
                                                                                  aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTGT‑TTTTGAAAAA‑ATAAATAAAA‑AATTTTTTTTTTAATATTGAGTTAAAGAAAAAAAAAAACACAAAAATAAAAATATTAAAGGATGGGAAGGAcaaaaaa                                                                    >  2:3492319/1‑149 (MQ=255)
                                                                                  aTCTGCTTTGTCGATACCACGTACATTTTAATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATTAAAT‑TATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                    <  2:2792899/149‑1 (MQ=255)
                                                                                    gtGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑TTTTCAAAAT‑ATAATGAATT‑TTTTTGTTTTTAATTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                                  <  2:192705/148‑1 (MQ=255)
                                                                                          tGTCGATACCAGGTAATTTTTCTTATCGTGTCGT‑TTGTTTTCG‑TTTTAAAAAT‑ATATTGAATT‑TATTTTTTTTTAATTATCGTTATAACTATCACAAAAACGACAATATGAAAATTATTCGTGGAGTGAAAGGCAAAAAAACGGCCTcc                                                            <  1:417876/149‑1 (MQ=255)
                                                                                           gTCTATACCACGTAAATTTTCATATCCTGTTTT‑TTTTTTTCT‑ATTTAAAATT‑ATAATCAAAT‑TATTTTTTTTTAAATATCTATATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTccc                                                           <  1:1690733/149‑1 (MQ=255)
                                                                                              gATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGG‑ATTTAAAAAT‑ATAATTAAAT‑TATTTGTTTTTTAATATAGAGATAAAGATCACAAAAAAGAAAAAATTAAAATTATTAGGTGGGGGTAATGGAAAAAAAAGGCCACCCgct                                                        >  2:2021854/1‑147 (MQ=255)
                                                                                               aaaCCACTAACATTTACATATCCGGGCTT‑TTGTTTCCT‑ATTACAAAAA‑ATAATCAAAT‑TATTTTTTTTTAAATATCGATATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                       <  2:4787106/147‑1 (MQ=255)
                                                                                                    aCGTACATTTTCATATCCTGTCGT‑TTTTTTTAG‑ATTTAAAAAT‑ATAATTTAAT‑TTTTTTTTTTTAAATTTCGTGATAAAGATAACAAAAAAGACAATTTGTAAATTTTTAGAGGTGGGTAAGGGCAAATAACGGCCTCCCGAGAgggga                                                  >  2:3410519/1‑147 (MQ=255)
                                                                                                     cGTACATTTTCATATCCTGTCGT‑TTGTTTTAG‑ATTTGAAAAT‑ATAATGAAAA‑TATTTTTTTTTAAATATCTCGATAACCATAACAAAAAAGTCAATATTAAATATATTCTGGGAGTGAAATGCAAAAAAAAGGCCACCCGATAGGGGAg                                                 >  2:2284561/1‑149 (MQ=255)
                                                                                                     cGTACATTTTCATATCCTGTCGT‑GTGTTTTAG‑ATTTAAAAAA‑ATAATTAAAT‑TATTTTTTTTTAAATATAGAGATAAAGATCACAAAAACGACAAAATTAAAATTATTCGAGGTGTGAAAGGCAAAAAAAACGCCTACCGATAGGGAAg                                                 >  2:812300/1‑149 (MQ=255)
                                                                                                      gTACATTTTCATATCCTGTCGT‑TTTTTTTCG‑ATTTCAAAAT‑ATATTGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                                <  2:3819118/149‑1 (MQ=255)
                                                                                                      gTACATTTTCATATCCTGTCGT‑GTTTTTTAG‑ATTTAAAAAT‑ATAATTAAAT‑TATTTTTTTTTAAATATCGAGATAAAGATAACAAAAACGACAAAAAGAAAATTATTAGAGGAGTGAAACGCAAAAAAAAGGCCACCCGAAATGGTAGc                                                >  2:773218/1‑149 (MQ=255)
                                                                                                       tACATTTTCATATCCTGTCGT‑TTGTTTTAG‑ATTTCAAAAA‑ATAATGAAAT‑TATTTTTTTTTAAATATCGAGATAACGATAACAAAAAAGACAAAAAGTAAATTATTTGAGGGGTGGAAGGCCAAAAAAACGCCGCCCCAAAGGGACGcc                                               >  2:2076720/1‑149 (MQ=255)
                                                                                                        aaaTTTTCATAGCCTGTCGT‑TTGTTTTCG‑ATTTAAAAAT‑ATAATGAAAT‑TATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                                              <  2:2889216/147‑1 (MQ=255)
                                                                                                          aTTTTCATATCCTGTCGT‑TTGTTTTAG‑ATTTAAAAAA‑ATAAATAAAA‑TATTTGTTTTTAAATATAGAGATAACGATCAAAAAAAAGAAAAAAATTAAAATATTAGAGGAGTGAAAGGCAAAAAAACCGCCAACCCATAGGGAAAcccga                                            >  2:416119/1‑146 (MQ=255)
                                                                                                          aTTTTCATATCCTGTCGT‑GTGTTTTAG‑ATTTAAAAAA‑ATAATTAAAT‑TATTTTTTTTTAAATATCGAGATAACGATCACAAAAAACACAAAATTAAAATTATTCGAGGAGTGAAAGGCCAAAAAAAGGCCACCCGATAGGGAAGCCgaa                                            >  2:2903829/1‑147 (MQ=255)
                                                                                                           ttttCATATCCTGTCGT‑TTGTTTTTG‑ATTTGAAAAA‑ATAATTAAAT‑TTTTTTTTTTTTTATTTAGTGTTTACGATAAAAAAAACGAGAATATTTAAATTATTGGTTGTGTGTTAGGGGAAAAAAATGACTGCCGAGAGGGAGTCCGttg                                           >  2:3642088/1‑147 (MQ=255)
                                                                                                                   tCCTTTCTT‑TTTTTTTCT‑ATTAAAAAAT‑ATAATTATTT‑TTTTTTTTTTAAATTATCTATAAAACTAACAAAACAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                                   <  1:1348954/149‑1 (MQ=255)
                                                                                                                          gt‑ttttttTCG‑ATTTCAAAAT‑ATATTGATTT‑TATTTTTTTTTAATTATGGATATATCTTTAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                            <  2:1190712/149‑1 (MQ=255)
                                                                                                                          gt‑ttgtttTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                            >  1:4062827/1‑149 (MQ=255)
                                                                                                                           t‑ttgtttTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgtgt                           >  1:3280699/1‑147 (MQ=255)
                                                                                                                           t‑ttgtttTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                           >  1:4745730/1‑149 (MQ=255)
                                                                                                                                ttttCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATCGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                       >  2:4052954/1‑149 (MQ=255)
                                                                                                                                   tCG‑ATTTCAAAAT‑ATAATTAAAT‑TATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                    <  2:2056954/149‑1 (MQ=255)
                                                                                                                                   tCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                    >  2:1842082/1‑149 (MQ=255)
                                                                                                                                     g‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAATCGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTTTGCCgg                  >  1:4558467/1‑149 (MQ=255)
                                                                                                                                     g‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                  >  1:2922927/1‑149 (MQ=255)
                                                                                                                                     g‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                  >  2:5000198/1‑149 (MQ=255)
                                                                                                                                       aTTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                 >  2:462229/1‑149 (MQ=255)
                                                                                                                                          tCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc              >  2:1712046/1‑149 (MQ=255)
                                                                                                                                            aaaaT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg            >  2:3883892/1‑149 (MQ=255)
                                                                                                                                            aaaaT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg            >  1:4125842/1‑149 (MQ=255)
                                                                                                                                                t‑ataATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa        >  2:3261712/1‑149 (MQ=255)
                                                                                                                                                  ataATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa       >  1:2030777/1‑149 (MQ=255)
                                                                                                                                                   taATTAAAT‑TATTTTTTTTTAAATATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa      <  1:1093930/149‑1 (MQ=255)
                                                                                                                                                   taATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa      >  1:4901031/1‑149 (MQ=255)
                                                                                                                                                   taATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa      >  1:4901076/1‑149 (MQ=255)
                                                                                                                                                     aTGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa    >  1:3718849/1‑149 (MQ=255)
                                                                                                                                                      tGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt   >  1:4028613/1‑149 (MQ=255)
                                                                                                                                                       gAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa  >  2:221050/1‑149 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
TTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATA  >  CP009273/2170576‑2170872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A