Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,724 G→T 36.7% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7240GT36.7% 230.3 / 60.2 180intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (47/60);  new base T (40/28);  total (89/91)
Fisher's exact test for biased strand distribution p-value = 6.36e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  CP009273/2170575‑2170873
                                                                                                                                                      |                                                                                                                                                      
tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATg                                                                                                                                                        <  1:2195236/150‑1 (MQ=255)
  tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                                      <  1:4232469/150‑1 (MQ=255)
  tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                                      <  1:1231072/150‑1 (MQ=255)
     accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTCAAAATATAATTAAatt                                                                                                                                                   >  2:1697004/1‑150 (MQ=255)
       caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAAATAAaatat                                                                                                                                                 >  2:2370889/1‑150 (MQ=255)
        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAattat‑t                                                                                                                                               <  1:3021804/150‑1 (MQ=255)
        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGATTTAAAAATATTATTAAaatat‑t                                                                                                                                               >  1:622489/1‑150 (MQ=255)
          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTg                                                                                                                                             <  1:1435710/150‑1 (MQ=255)
          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTg                                                                                                                                             <  1:1463306/150‑1 (MQ=255)
          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTg                                                                                                                                             <  2:239962/150‑1 (MQ=255)
          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTg                                                                                                                                             <  1:895905/150‑1 (MQ=255)
          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTg                                                                                                                                             <  2:1909909/150‑1 (MQ=255)
            cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGAATTTCAAAAATTATTAAAAtttt‑tttt                                                                                                                                            >  1:3720255/1‑148 (MQ=255)
             ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGttt                                                                                                                                          <  2:1162596/150‑1 (MQ=255)
             ttGCATCGTTTAGAGATTGTTAATATTGAATGCCTGAACCTCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGttt                                                                                                                                          <  2:1162512/150‑1 (MQ=255)
              tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTATATTTAAAAAAATAATAAAAAAAt‑ttttttt                                                                                                                                         >  1:3319944/1‑150 (MQ=255)
               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTGGATTTCAAAAAATAAATAAATTAT‑tttttttt                                                                                                                                        >  2:1119086/1‑150 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTCGATTTCAAAATATAATTAAAATAT‑TTTTTTTTAAata                                                                                                                                   >  2:1772866/1‑150 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTAAAAATATAATGAAATTAT‑TTGTTTTTAAata                                                                                                                                   >  2:1772703/1‑150 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTAAAAAAATAAATAAAATATATTTTTTTTaaaa                                                                                                                                    >  1:1724977/1‑149 (MQ=255)
                     tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAatat                                                                                                                                  <  2:4176620/150‑1 (MQ=255)
                     tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAatat                                                                                                                                  <  2:1706476/150‑1 (MQ=255)
                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATc                                                                                                                                 <  2:3172030/150‑1 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTCGATTTAAAAATATAATTTAATTAA‑TTTTTTTTAAatataca                                                                                                                               >  2:3075640/1‑147 (MQ=255)
                         gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTATATTTAAAAAAATAAATAAAAAAT‑TTTTTTTTTAaaataaat                                                                                                                              >  2:3648954/1‑146 (MQ=255)
                          agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCgaga                                                                                                                             <  2:3317504/150‑1 (MQ=255)
                            aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTTTATTTCAAAAAAAAATGAAAATAA‑TTTTTTTTTAatatatatata                                                                                                                           >  1:229970/1‑143 (MQ=255)
                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAGAATTGAAAAAAAAAATAAATTAT‑TTTTTTTTAAATATAGAGaaaa                                                                                                                          >  2:3399254/1‑150 (MQ=255)
                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATaa                                                                                                                          <  1:3001535/150‑1 (MQ=255)
                             ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTTTTTTTAAAAAAAAAAATAAAATAA‑TTTTTTTTTTATATATAGATaa                                                                                                                          >  1:3786297/1‑150 (MQ=255)
                              tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAAc                                                                                                                         <  2:3720598/150‑1 (MQ=255)
                              tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAAc                                                                                                                         <  2:2486686/150‑1 (MQ=255)
                               gtgAATATTGCATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACg                                                                                                                        <  1:4166594/150‑1 (MQ=255)
                               gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTTGATTTAAAAAAATAAATTAATTAA‑TTTTTTTTAATTATAGAGATaaag                                                                                                                        >  1:3135745/1‑148 (MQ=255)
                               gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATTAAAATAT‑TTTTTTTTAAATATAGAGATaaag                                                                                                                        >  2:1403288/1‑148 (MQ=255)
                                tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGa                                                                                                                       <  1:3405943/150‑1 (MQ=255)
                                 gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGTTTTGAAAAAATAAATAAAATAT‑TTTTTTTTTTATATAGTGGTAACGAt                                                                                                                      >  1:3716891/1‑150 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATc                                                                                                                     <  2:3172288/150‑1 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATc                                                                                                                     <  1:1704603/150‑1 (MQ=255)
                                   atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGATATTAT‑TTGTTTTTAAATATCGAGATAACGATca                                                                                                                    <  2:3479080/150‑1 (MQ=255)
                                   atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATca                                                                                                                    <  2:1499638/150‑1 (MQ=255)
                                       tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAAATAT‑TTGTTTTTAAATATAGAGAGAACGATCACaaa                                                                                                                >  2:2821439/1‑150 (MQ=255)
                                        gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATTAAATTAT‑TTTTTTTTAAATATCGAGATAACGAACACaaaa                                                                                                               >  2:3627076/1‑150 (MQ=255)
                                           tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACg                                                                                                            <  2:3235713/150‑1 (MQ=255)
                                           tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTATATTTATAAATAAAAATAAAATAA‑TTTTTTTTTTATATAGAGTTACAGAGAACAAAAAca                                                                                                            >  1:4036753/1‑149 (MQ=255)
                                            gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                           <  2:2101935/150‑1 (MQ=255)
                                                gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGGTTTAAAAAAATAAATAAAATTT‑TTTTTTTTTAATATATAGATAAAGATaaaaaaaaacaccaa                                                                                                       >  1:3320505/1‑143 (MQ=255)
                                                   ccGAATAACCGCCGGGGTGTGGGTTTTTCAGAATGTGTTTTTTCTATACAACTTAATTTTTCTTATCGTGTGGT‑TTTTTTTCTTTTTAAAAATATATTTAATTTTT‑TTTTTTTTAAATTTGGATATAACTATCACAAAAACGACAATATg                                                                                                    <  2:4103777/150‑1 (MQ=255)
                                                     gCATAACCGCGGCGCTGTGCTTTGTTCAGCTTCTGGTTTGTCTATACCACTTACTTTTTCATATCCTGTCGT‑TTTTTTTCGTTTTCAAAATATATTGAAATTAT‑TTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaa                                                                                                  <  2:3872800/150‑1 (MQ=255)
                                                      cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTAAAAAAAAAAAGAAATTAT‑TTTTTTTTTTATATAGAGATTAAGCTCACAAAAAAGACAaaatttaa                                                                                                 >  2:2225282/1‑146 (MQ=255)
                                                        tacccgccgCGGTGTGCGTTGTTCAGAATGTGCTTTGTCTATACCACGAAATTTTTCATATCCTGTGTT‑TTTTTTTCTATTTAAAAATATTATTAATTTAT‑TTTTTTTTAAATATCTAGATACCTATCAAAAAAACGACAATATGAAAat                                                                                               <  2:2182354/147‑1 (MQ=255)
                                                        tacccgccgCGCTGTGGGTTTTTCAGCATCTGCTTTGTCGATACCCCGTACATTTTCATATCCTGTCTT‑TTTTTTTCTATTTCAAAATATAATGAATTTAT‑TTTTTTTTAAATATCGATATAACGATCACAAACACGACAATATGAAAat                                                                                               <  2:2181725/147‑1 (MQ=255)
                                                        tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTAAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCAAAAAAACGACAATATGAAAat                                                                                               <  2:1422499/150‑1 (MQ=255)
                                                         aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTGGATTTGAAAAAAAAATTAAAATAT‑TTTTTTTTTAAAATAGAGATAAACAACACAAAAAAAACaaaattaaaatt                                                                                              >  1:2365189/1‑150 (MQ=255)
                                                         aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGGTTTAAAAAAAAAATTTAATTAT‑TTTTTTTTAAATATAGAGGTTACGAACACAAAAAAGACAAAATGagaaat                                                                                              >  1:3572728/1‑148 (MQ=255)
                                                         aaCCGCCGCGCTGTGCGTTGTCCAGCATCTGCTTTGTCTATACCACGTACTTTTAAATATCCTTTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatt                                                                                              <  2:4335389/150‑1 (MQ=255)
                                                                  gcTGGGCGTGGTACAGCATCTGCTTTTTCGATACCACTTACTTTTTCATATCCTGTCTT‑TTTTTTTCTATTTAAAAATATAATCAATTTAT‑TTGTTTTTAAATATCGATATAACGACCACAAAAACGACAATATGAAAATTATTCgagga                                                                                     <  2:20638/150‑1 (MQ=255)
                                                                    tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATTAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGt                                                                                   <  2:649562/150‑1 (MQ=255)
                                                                     gtgCGTTGTTCAGCATCTGCTTGGTCGATACCACGTACATTTTCATATCCGGTCGT‑TTGTTTTCGATTTCAAAATATAATTAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTg                                                                                  <  2:1351616/150‑1 (MQ=255)
                                                                      tgCGTTGTTCAGCATCTGCTTGGTCGATACCACGTACATTTTCATATCCTGGCGT‑TTTTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGa                                                                                 <  2:1010578/150‑1 (MQ=255)
                                                                            gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTAGATTTAAAAAAAAAAATAAAATAT‑TTTTTTTTAAATATCGAGATAACGATAACAAAAAAGAAAAAAAGAAAAATATTAGAGGAGTTGAAGGCa                                                                           >  2:1692722/1‑150 (MQ=255)
                                                                             ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGATTTAAAAAAAAAATTAAAAAAA‑TTTTTTTTTAATATAGAGAAAAACAAAACAAAAAAAACAAAAAGAAAATTAATAGAGGACTGTAAGGCaa                                                                          >  1:1366525/1‑150 (MQ=255)
                                                                              tCAGAATCTGATTTGTCGATACCACGTAATTTTTCATATCCTGTCGT‑TTTTTTTCGTTTACAAAATATAATGAATTTAT‑TTTTTTTTAAATATCGATATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                                         <  1:2799130/150‑1 (MQ=255)
                                                                                      tgttttGTCGATCCCCCGTAAATTTTCATATCCTGTGTT‑TTTTTTTCTTTTAAAAAATATAATTAATTTTT‑TTTTTTTTAAATATCGATATAACGAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                                 <  1:3890938/147‑1 (MQ=255)
                                                                                         tttGTCTATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTCGATTTCAAAATATAATTAAATTAT‑TTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTc                                                              <  2:355657/150‑1 (MQ=255)
                                                                                              cGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTAAAAATATAATTAAATTAT‑TTTTTTTTTAATATCGAGATAAAGATAACAAAAAAGACAATATTAAAATTATTGGAGGAGTGTAAGGGAAAAAAAAGGCCTCCCGaa                                                         >  2:3568260/1‑149 (MQ=255)
                                                                                              cGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGATTTCAAAATATAATGAAAATAT‑TTTTTTTTAAATATCGAGTTAACGATCACAAAAACGACACTATGAAAATTATTAGAGGGGTGAAAGGCCAAAAAACCGCCACCCGag                                                         >  2:1933523/1‑149 (MQ=255)
                                                                                                 tACCACTTAAATTTTCATATCGTGTCTT‑TTTTTTTCTATTAAAAAATATAATAAAATTAT‑TTTTTTTTAATTATCTATATAACTATCAAAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                                      <  1:3477800/150‑1 (MQ=255)
                                                                                                  aCCACGTACATTTTCATATCCTGTCGT‑TTTTTTTGGGTTTAAAAATATAATGAAATTAT‑TTTTTTTTTAATATAGAGATAAAATTAAAAAAAACGAAAATATTAAAATTATTAGTGGGGTGGATTGTCAAAAAAAGGCCGGCCGAGAggg                                                     >  2:3989717/1‑150 (MQ=255)
                                                                                                  aCCACGTACATTTTCATATCCTGTCGT‑GTGTTTTTGATTTGAAAATATAATTAAAATAT‑TTTTTTTTTAATTTAGAGTTTACGATAACCAAAACGACAATATTAAAATTTTTAGGGGGGGGTAATGGAAAAAAAAGGCCTGCCGCTTggg                                                     >  2:4163751/1‑150 (MQ=255)
                                                                                                  aCCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGTTTTGAAAAAATAATTTAAATAT‑TTTTTTTTTAATATGGAGATTAACATGACAAAAAAGACCAAATTAAAATTATTTGGGGGGGGTAATGCCAAAAAAAGGCCGCCCGGTTggg                                                     >  2:571123/1‑150 (MQ=255)
                                                                                                  aCCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCGATTTAAAAATATAATGAAATTAT‑TTTTTTTTATATATCGAGGTAAAGATCCCAAAAACGACAATATGTAAATTATTGGAGGAGTGGAAGGCAAAAAAAAAGCCACCCGCGAggg                                                     >  2:2739579/1‑150 (MQ=255)
                                                                                                    cACTTACTTTTTCTTATCTTGTCTT‑TTTTTTTCTTTTTAAAAATATAATTAATTTTT‑TTTTTTTTAAATATCTTGATACCGAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                   <  1:1015078/150‑1 (MQ=255)
                                                                                                    cACGTAAATTTTCATATCCTGTCTT‑TTTTTTTATATTTAAAAATATAATTAAATTAT‑TTTTTTTTAAATATCTATATAACTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                   <  1:320681/150‑1 (MQ=255)
                                                                                                      cGTACATTTTCATATCCTGTCGTGTTGTTTT‑GATTTAAAAATAAAATGAAAATAT‑TTTTTTTTTAAAATAGAGATAACGATAAAAAAAAACACCAAAATAAAATTATTAGGGGTGTGGAAGGCAAAAAAAACGCCACCCCAGAGGGGAGc                                                 >  2:780183/1‑150 (MQ=255)
                                                                                                       gTAAATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                                <  2:258000/150‑1 (MQ=255)
                                                                                                          cATTTTCATATCCTGTCGT‑GTGTTTTGGTTTTAAAAATATAATGAAAATAT‑TTTTTTTTTAATATAGAGTTAACGATCACAAAAAAGACAAAATGAAAATTTTTAGAGGGGTGTGAGGGAAAAAAAAAGCCTCCCGAGAGGGACGCCGTa                                             >  2:1188035/1‑150 (MQ=255)
                                                                                                                aatatCCTGTCTT‑TTTTTTTCTATTTAAAAATATAATTAATTTAT‑TTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAg                                       <  1:258054/149‑1 (MQ=255)
                                                                                                                  tatCCTGTGGT‑TTTTTTTCTTTTAAAAAATATATTTAATTTTT‑TTTTTTTAAAATATCGTTATACCCAAAACAACAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                                     <  1:1209436/150‑1 (MQ=255)
                                                                                                                        gTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGAAAATATGAAAATTATTCGAGTTGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                               >  2:3891515/1‑150 (MQ=255)
                                                                                                                          ctt‑ttgtttTCTATTAAAAAATATAATTAATTTAT‑TTTTTTTTAAATATCGAGATAACGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                             <  2:4041017/148‑1 (MQ=255)
                                                                                                                          cGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                             >  1:2076831/1‑150 (MQ=255)
                                                                                                                           gt‑ttgtttTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                            >  1:4212708/1‑150 (MQ=255)
                                                                                                                            t‑ttgtttTCGATATCAAAAAATAATGAAAATAT‑TTGTTTTTAAATAACGAGATAACGATCACAAAAACGACAATATGAAAATAATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCCATATGGAAGCCGTAGCAAAGAGCGAGTGttat                           >  1:3239958/1‑148 (MQ=255)
                                                                                                                                 ttttCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                       >  1:1871674/1‑150 (MQ=255)
                                                                                                                                   ttCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAGATTATTGGTGGAGTGTAAGGCAATAAAACGGCGTCGGGATGGGGTAGCCGTAGCTAGGTGCGGGGGtttgtttggg                     >  1:2194285/1‑143 (MQ=255)
                                                                                                                                      gATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                  <  2:895190/150‑1 (MQ=255)
                                                                                                                                          tCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg              >  2:1532058/1‑150 (MQ=255)
                                                                                                                                           cAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             >  2:3778258/1‑150 (MQ=255)
                                                                                                                                           cAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             >  2:1197641/1‑150 (MQ=255)
                                                                                                                                           cAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             >  1:3516482/1‑150 (MQ=255)
                                                                                                                                           aaaaaTATAATTAATTTAT‑TTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg             <  1:2099474/149‑1 (MQ=255)
                                                                                                                                            aaaaTATAAGGAAATTAT‑TTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            <  2:2167928/150‑1 (MQ=255)
                                                                                                                                               atatAATGAAATTAT‑TTGTTTTTAAATATCGAGATACCTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa         <  2:1599636/150‑1 (MQ=255)
                                                                                                                                               atatAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAGGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa         >  1:4254181/1‑150 (MQ=255)
                                                                                                                                               atatAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa         <  2:3786297/150‑1 (MQ=255)
                                                                                                                                               atatAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa         >  2:2611032/1‑150 (MQ=255)
                                                                                                                                                tataATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa        >  1:377334/1‑150 (MQ=255)
                                                                                                                                                  taATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc      >  1:1565843/1‑150 (MQ=255)
                                                                                                                                                  taATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc      >  2:2681361/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAT‑TTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  CP009273/2170575‑2170873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A