Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,724 G→T 23.4% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7240GT23.4% 576.1 / 37.9 272intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (79/114);  new base T (48/23);  total (131/141)
Fisher's exact test for biased strand distribution p-value = 1.57e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 278 total aligned reads displayed.

TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  CP009273/2170575‑2170873
                                                                                                                                                       |                                                                                                                                                     
tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑Tg                                                                                                                                                       <  1:1554646/150‑1 (MQ=255)
    tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAat                                                                                                                                                   <  2:317358/150‑1 (MQ=255)
     accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAatt                                                                                                                                                  <  1:3153065/150‑1 (MQ=255)
     accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAatt                                                                                                                                                  <  1:3097388/150‑1 (MQ=255)
     accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAatt                                                                                                                                                  <  2:2401983/150‑1 (MQ=255)
      ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAatta                                                                                                                                                 <  1:1088708/150‑1 (MQ=255)
      ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATATAA‑‑TTAAaata                                                                                                                                                 >  2:2712032/1‑150 (MQ=255)
        accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAattatt                                                                                                                                               <  1:600452/150‑1 (MQ=255)
         ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTAttt                                                                                                                                              <  2:4736784/150‑1 (MQ=255)
         ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTAttt                                                                                                                                              <  2:2119631/150‑1 (MQ=255)
          cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTg                                                                                                                                             <  1:2562379/150‑1 (MQ=255)
           acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGt                                                                                                                                            <  1:5119907/150‑1 (MQ=255)
               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGttttt                                                                                                                                        <  1:3783970/150‑1 (MQ=255)
               gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAA‑‑ATAAATTATtttttttt                                                                                                                                        >  1:1184794/1‑150 (MQ=255)
                cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAAAATAA‑‑TTAAATTATTTGTTTTTa                                                                                                                                       >  2:2546418/1‑150 (MQ=255)
                   cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAat                                                                                                                                    <  1:2628622/150‑1 (MQ=255)
                   cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGATTTAAAAAAAAAA‑‑TTAAAATTTTTTTTTTTTAat                                                                                                                                    >  1:16084/1‑150 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGGTTTAAAAAAAAAAATTAAAAATATTTTTTTTTaaa                                                                                                                                     >  1:1389083/1‑150 (MQ=255)
                    gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAata                                                                                                                                   <  1:282279/150‑1 (MQ=255)
                     tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAatat                                                                                                                                  <  1:3888591/150‑1 (MQ=255)
                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATc                                                                                                                                 <  1:4770536/150‑1 (MQ=255)
                      ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATc                                                                                                                                 <  2:4382555/150‑1 (MQ=255)
                       tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCAGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCg                                                                                                                                <  2:2128420/150‑1 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGCATTCAAAAAATAA‑‑TGAAAATATTTTTTTTTAAATATCga                                                                                                                               >  2:5002391/1‑150 (MQ=255)
                        cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCga                                                                                                                               <  1:733262/150‑1 (MQ=255)
                         gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCgag                                                                                                                              <  1:790653/150‑1 (MQ=255)
                         gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCgag                                                                                                                              <  1:1241669/150‑1 (MQ=255)
                          agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATATAA‑‑ATAAAATATTTTTTTTTTAATATAgata                                                                                                                             >  1:2907432/1‑148 (MQ=255)
                          agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAAAAA‑‑TTAAAAAAATTTTTTTTTAatatatata                                                                                                                             >  1:3627541/1‑145 (MQ=255)
                            aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATa                                                                                                                           <  1:964754/150‑1 (MQ=255)
                            aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATa                                                                                                                           <  2:172624/150‑1 (MQ=255)
                            aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGGTTTCGAATTCAAAATATAA‑‑AGAAATAATTTTTTTTTAAATATAGAtata                                                                                                                           >  2:3024741/1‑150 (MQ=255)
                              tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                                         <  1:4167344/150‑1 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                                     <  1:1469474/150‑1 (MQ=255)
                                  aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTAATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                                     <  1:3041298/150‑1 (MQ=255)
                                   atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                                    <  1:3496473/150‑1 (MQ=255)
                                    tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATATAA‑‑TTAAATTATTTTTTTTTTTATATATATATAAAGATcaa                                                                                                                   >  1:1999593/1‑149 (MQ=255)
                                     atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                                  <  2:1294926/150‑1 (MQ=255)
                                           tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACg                                                                                                            <  2:3883191/150‑1 (MQ=255)
                                              cGGAACCGCATACCCGCCGCGGTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACa                                                                                                         <  2:1105357/150‑1 (MQ=255)
                                              cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAA‑‑TTAAATTATTTTTTTTTTAATATAGAGATTAAGATAACAAAAACGACa                                                                                                         >  1:5225769/1‑150 (MQ=255)
                                               ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAAAATAA‑‑TGAAATTAATTTTTTTTTAATATGGAGGTAACGATCACaacaacttcaa                                                                                                        >  1:1880928/1‑145 (MQ=255)
                                                     gcttAACCGCCGCGCTTTGCGGTGTTCAGCATCTGCTTTGTCGATACCACTTACTTTTTCATATCCTGTCGTTTGTTTTCTTTTTCAAAATATAT‑‑TGAAATTATTTGTTTTTAAATATCGTGATAACGATCAAAAAAACGACAATATGaa                                                                                                  <  2:4431494/147‑1 (MQ=255)
                                                      cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGTTTTAAAAAAATAA‑‑ATAAAATAATTTTTTTTTTATATATGGATAAAGATAAAAAAAAAAACAAAAAGaaa                                                                                                 >  1:459676/1‑150 (MQ=255)
                                                       aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTGAAAAAAAAA‑‑ATAAATTATTTTTTTTTTTATATAGTTATGACAGTAAAAAAAAAAACAAAATGaaaa                                                                                                >  1:4108384/1‑150 (MQ=255)
                                                              ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCg                                                                                         <  2:3694869/150‑1 (MQ=255)
                                                                 cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagg                                                                                      <  2:426732/150‑1 (MQ=255)
                                                                   cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAA‑‑TGAAATTATTTTTTTTTAAATATAGAGATAAAGATAACAAAAAAGACAAAATTTAAATAATTGgaggtg                                                                                    >  2:3124542/1‑148 (MQ=255)
                                                                   cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTAAAAAAATAA‑‑TTTAAATATTTTTTTTTTAATATAGAGATAAAGATAAAAAAAAAGAAAAAATTAAAaatattagtggtg                                                                                    >  1:5169399/1‑143 (MQ=255)
                                                                    tgtgCGTTTTTCAGCATCTGCTTTGTCGATACCCCGTACATTTACATATCCGGTCTTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGt                                                                                   <  2:3627541/150‑1 (MQ=255)
                                                                    tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTGAAAATATAA‑‑TTAAATTATTTTTTTTTTAAAATAGAGGTAAAGATAACAAAAACTAAAAGATGTAAATTATTAgaggagg                                                                                   >  2:1937263/1‑149 (MQ=255)
                                                                      tgCGTTGTCCCGCATCTGCTTTGTCGATACCCCTTACTTTTTAATATCCTGTCTTTTGTTTTCTATTACAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGa                                                                                 <  1:4354656/150‑1 (MQ=255)
                                                                            gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAA‑‑ATAAAATATTTTTTTTTAAATATAGAGATAAAGATAACAAAAAAGACAATATGAAAATTATTAGAGGAGGGAAAGGCa                                                                           >  2:2503819/1‑150 (MQ=255)
                                                                                   aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAA‑‑TTTAATTTTTTTTTTTTTAATATAGAGATAAAGATAAAAAAAAAGACAATATTAAAAATATTAGAGGTGTGAAAGGCaaaaaaaa                                                                    >  2:2655251/1‑149 (MQ=255)
                                                                                   aTCTGCTTTGCCTATACCACGTACATTTTAATATCCTGTCTTTTTTTTTCTATTTCAAAATATAA‑‑TTAATTTATTTTTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                    <  1:228523/150‑1 (MQ=255)
                                                                                     cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAAAAAACAAAAAAAAA‑‑TGAAAATATTAGTTTTTAAAAATCAAAATAAAAGTCACAAAAAAGAAAATAAGAAAAAAATTAAAAGAATGAAATGaaaaaaaaacg                                                                  >  2:2451845/1‑147 (MQ=255)
                                                                                      tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAA‑‑ATAAAATATTTTTTTTTTAATATATAGATAAACATAAAAAAAAACAAAAAATTAAAAATATTAGAGGAGGGTAAGGCaaaaaaaaagc                                                                 >  2:2291482/1‑146 (MQ=255)
                                                                                       gatttGTCGATACAACTTAAATTTTCATATATTGTGGTTTTTTTTCTTTTTAAAAATTTAA‑‑TGAATTTTTTTTTTTTTAAATTGCTATATAACGATCACAAAAACGAAAATATGAAAATTATTCGAGGAGTTAAAGGCAAAAAAACGGcc                                                                <  2:3993747/148‑1 (MQ=255)
                                                                                         tttGTCTATACCACTTAATTTTTCATATCTTTTTTTTTTTTTTCTTTTAAAAATTATAT‑‑TTATTTTTTTTTTTTTTAAATTTCTTTATAACTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTc                                                              <  1:1706476/150‑1 (MQ=255)
                                                                                            gTCGATACCACGTAAATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TTAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCg                                                           <  2:4677139/150‑1 (MQ=255)
                                                                                               gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTACAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                        <  2:3973570/150‑1 (MQ=255)
                                                                                                aTACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGGTTTAAAAATATAA‑‑ATAAAATATTTTTTTTTTAATATAGAGATAAAGATAACAAAAACGACAATATTAAAATTATTAGAGGGGTGAAAGGGAAAAAAAAGGCCACCCGATAg                                                       >  1:407978/1‑150 (MQ=255)
                                                                                                    cACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTTAAAAAATAA‑‑ATTAAATATTTTTTTTTTAATAAAGAGGTAAAGGTCAACAAAAAGACAAAAATAAAATTATTAGAGGGGTGGAAGGGAAAAAAAAGGCCGCCCCAGAgggga                                                   >  2:4026818/1‑148 (MQ=255)
                                                                                                    cACGTACATTTACATATCCTGTCGTTTGTTTTCTATTACAAAATATAA‑‑TGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                                   <  1:4442698/150‑1 (MQ=255)
                                                                                                      cGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAA‑‑TGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATTAAAAATATTAGAGGAGTGAAAGGCAAAAAAACGGCCACCCCAGAGGGAAGc                                                 >  2:1651993/1‑150 (MQ=255)
                                                                                                       gTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAA‑‑TTTAAATATTTTTTTTTTAATATAGAGATAAAGATAACAAAAAAGACAATATTAAATATATTAGTGGAGTGTAATGCAAAAAAAAGGCCGCCCGATAGGGTAGcc                                                >  2:82567/1‑150 (MQ=255)
                                                                                                          atttttCATATCGTGTTTTTTTTTTTCTTTTAAAAAATATAA‑‑TTAATTTTTTTGTTTTTAAATATCTACAAACCGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                             <  1:2409162/148‑1 (MQ=255)
                                                                                                            ttttCATATCCTGACGTTTGTTTTCGATTACAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGc                                           <  2:4873296/150‑1 (MQ=255)
                                                                                                                 atatCCTGTCGTTTGTTTTCGATTTAAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGt                                      <  2:790337/150‑1 (MQ=255)
                                                                                                                  tatCCTGTCGTTTGTTTTATAATTAAAAAAAAAA‑‑ATAAAATATTTTTTTTTTAATATAGATATAAAGATAAAAAAAAAAAAAAAAATAAAATTAATAGAGGAGTGAAAGGCAAAAAAACGGCCACCCGCTAGGGAAACCCAAGCacaatg                                     >  2:3278839/1‑145 (MQ=255)
                                                                                                                       tGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                                >  2:3674895/1‑150 (MQ=255)
                                                                                                                        gTCGTTTGTTTTCGAATTCAAAATATAA‑‑TTAAAATAATTTTTTTTAAATATAGATATAACGATAAAAAAAACGACAAAAATAAAATTATTAGATGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGGAGCCGTAGCAAAGTGCGCgtg                               >  1:3714477/1‑150 (MQ=255)
                                                                                                                         gcGTTTGTTTTCTATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                              <  2:5265570/149‑1 (MQ=255)
                                                                                                                           gtttgtttTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                            >  2:1120913/1‑150 (MQ=255)
                                                                                                                            tttgtttTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                           >  2:3159929/1‑150 (MQ=255)
                                                                                                                             ttgtttTCGATTTCAAAATATAC‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                          <  2:2828806/150‑1 (MQ=255)
                                                                                                                              tgtttTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                         >  1:3307865/1‑150 (MQ=255)
                                                                                                                               gtttTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                        >  1:3135306/1‑150 (MQ=255)
                                                                                                                               gtttTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                        >  2:3395845/1‑150 (MQ=255)
                                                                                                                                ttttataTTTAAAAATATAA‑‑TTATATTATTTTTTTTTAAATATCTATAAAACGAAAAAAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                       <  1:2372437/144‑1 (MQ=255)
                                                                                                                                ttttCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                       >  2:4487551/1‑150 (MQ=255)
                                                                                                                                  ttCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                     >  2:3355812/1‑150 (MQ=255)
                                                                                                                                     gATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATCGGGAAGCCGTAGCAAAGTGCGCGTGTTGTTATGCCGGa                  >  2:1527815/1‑150 (MQ=255)
                                                                                                                                      aTTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                 >  2:4588695/1‑150 (MQ=255)
                                                                                                                                      aTTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCGCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                 <  2:575826/150‑1 (MQ=255)
                                                                                                                                         aaaaaaaaTAA‑‑TTAAATTTTTTGTTTTTAAATTTCTATATAACTAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg              <  1:720619/148‑1 (MQ=255)
                                                                                                                                          cAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTGTTTATGCCGGATGCgg             >  1:4686599/1‑150 (MQ=255)
                                                                                                                                           aaaaTATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            >  2:49611/1‑150 (MQ=255)
                                                                                                                                           aaaaTATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt            >  1:4484344/1‑150 (MQ=255)
                                                                                                                                            aaaTATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg           >  2:3555256/1‑150 (MQ=255)
                                                                                                                                             aaTATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt          >  2:608361/1‑150 (MQ=255)
                                                                                                                                              atatAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAATGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGAGAGAGAGTGTTTAAGCCGGATGCGGTGTa         >  1:3966065/1‑150 (MQ=255)
                                                                                                                                              atatAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa         >  2:3715755/1‑150 (MQ=255)
                                                                                                                                              atatAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa         >  1:1481591/1‑150 (MQ=255)
                                                                                                                                                ataA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa       >  2:1235013/1‑150 (MQ=255)
                                                                                                                                                 taA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc      >  2:1348289/1‑150 (MQ=255)
                                                                                                                                                 taA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc      >  1:1842344/1‑150 (MQ=255)
                                                                                                                                                  aa‑‑ttaattTTTTTTTTTTAAAATATCTATATAACTATAACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa     <  1:2709170/150‑1 (MQ=255)
                                                                                                                                                      tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa   >  2:979339/1‑150 (MQ=255)
                                                                                                                                                       gAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAc  <  2:407978/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
TTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATAC  >  CP009273/2170575‑2170873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A