Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F5 I0 R1 92 232.7 8849178 85.5% 7566047 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,724 G→T 25.3% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7240GT25.3% 297.3 / 26.4 166intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (48/66);  new base T (28/15);  total (80/86)
Fisher's exact test for biased strand distribution p-value = 1.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

TTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAT  >  CP009273/2170576‑2170871
                                                                                                                                                     |                                                                                                                                                     
 tcgaccaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGa                                                                                                                                                      <  1:2721691/146‑1 (MQ=255)
  cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGaa                                                                                                                                                     <  1:3624646/149‑1 (MQ=255)
  cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGaa                                                                                                                                                     <  1:3624649/149‑1 (MQ=255)
   tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGaaa                                                                                                                                                    <  2:2642790/149‑1 (MQ=255)
    accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAat                                                                                                                                                   <  2:3970511/149‑1 (MQ=255)
    accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAat                                                                                                                                                   <  2:1840715/149‑1 (MQ=255)
     ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAAT‑AAATAAatt                                                                                                                                                  >  2:840047/1‑149 (MQ=255)
      caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGTTTTCAAAATAT‑aattaaatta                                                                                                                                                 >  2:1433196/1‑149 (MQ=255)
       accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAattat                                                                                                                                                <  2:3445946/149‑1 (MQ=255)
       accacTTGCATCGTATCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAattat                                                                                                                                                <  2:4027281/149‑1 (MQ=255)
        ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATAT‑aattaaattaat                                                                                                                                               >  1:3812195/1‑147 (MQ=255)
        ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAattatt                                                                                                                                               <  2:1111951/149‑1 (MQ=255)
        ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAattatt                                                                                                                                               <  2:2185493/149‑1 (MQ=255)
         cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTAttt                                                                                                                                              <  1:861271/149‑1 (MQ=255)
         cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTAttt                                                                                                                                              <  1:52481/149‑1 (MQ=255)
         cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTAttt                                                                                                                                              <  2:2496962/149‑1 (MQ=255)
         cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTAttt                                                                                                                                              <  1:2777409/149‑1 (MQ=255)
           cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAAT‑AATTAAAATAttttt                                                                                                                                            >  2:3729156/1‑147 (MQ=255)
            ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGtt                                                                                                                                           <  2:904053/149‑1 (MQ=255)
            gtGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGtt                                                                                                                                           <  2:3113150/148‑1 (MQ=255)
               cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATAT‑AAATAAATTATtttttttt                                                                                                                                        >  2:4233110/1‑149 (MQ=255)
               cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGttttt                                                                                                                                        <  2:1520408/149‑1 (MQ=255)
                aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTa                                                                                                                                       <  1:3632154/149‑1 (MQ=255)
                 tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTGATTTAAAAATAT‑AATTAAAtttttttttttttt                                                                                                                                      >  1:2770285/1‑147 (MQ=255)
                 tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAAT‑AATGAAATTATttttttttta                                                                                                                                      >  2:1757782/1‑147 (MQ=255)
                 tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAAT‑AAATAAATTATTTTTTTTTaa                                                                                                                                      >  1:2771344/1‑149 (MQ=255)
                   gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAat                                                                                                                                    <  1:1703439/149‑1 (MQ=255)
                   gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAAT‑AATTAAAATATTTTTTTTTAAat                                                                                                                                    >  2:4207351/1‑149 (MQ=255)
                   gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTAGATTTTAAAAAAA‑AAAAAAAATATTTTTTTTTtaaa                                                                                                                                    >  1:1506671/1‑148 (MQ=255)
                    tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAata                                                                                                                                   <  1:995618/149‑1 (MQ=255)
                    tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAata                                                                                                                                   <  1:3311312/149‑1 (MQ=255)
                     ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAatat                                                                                                                                  <  2:4315258/149‑1 (MQ=255)
                     ttCGAGATAGAGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAatat                                                                                                                                  <  2:2715123/149‑1 (MQ=255)
                      tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATc                                                                                                                                 <  1:4374107/149‑1 (MQ=255)
                      tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATc                                                                                                                                 <  1:2381786/149‑1 (MQ=255)
                        gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTGATTTAAAAAAAA‑AATGAAATTATTTTTTTTTAAaaatata                                                                                                                               >  1:2129074/1‑146 (MQ=255)
                        gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAATAA‑AATGTAAATAATTTTTTTTAAATATCga                                                                                                                               >  1:2130093/1‑149 (MQ=255)
                         agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAAAAA‑AAATAAAATATTTTTTTTTAAATATAgaa                                                                                                                              >  1:651819/1‑148 (MQ=255)
                         agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAAA‑AAATAAATTATTTGTTTTTAAATATagag                                                                                                                              >  2:1340175/1‑149 (MQ=255)
                         agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTAGTTTTAAAAAAAT‑AATGAAATTATTTTTTTTTAAATATagag                                                                                                                              >  1:651762/1‑149 (MQ=255)
                            ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTATATTTAAAAATAA‑AAATAAAATATTTTTTTTTTAATATAGAGATa                                                                                                                           >  2:28695/1‑149 (MQ=255)
                                gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGTTTTGAAAATAT‑AATTAAATTATTTTTTTTTTAATATAGAGATAACGa                                                                                                                       >  1:3126775/1‑149 (MQ=255)
                                gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGTTTTAAAAAAAT‑AAATAAATTATTTTTTTTTTAATATAGAGTTaaaaa                                                                                                                       >  1:1782074/1‑146 (MQ=255)
                                 aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                      <  2:3772621/149‑1 (MQ=255)
                                  atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTTTTTTTTAATATAGAGATAAAGAta                                                                                                                     >  2:1070283/1‑148 (MQ=255)
                                        aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTAGATTTCAAAATAA‑AATGTAATTAATTTTTTTTTAATATAGAGCTAACGATCAC‑aaaa                                                                                                              >  2:3387555/1‑149 (MQ=255)
                                         aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑aaaaa                                                                                                             <  2:4416615/149‑1 (MQ=255)
                                         aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATAT‑AATGTAATTATTTTTTTTTTAATATAGAGATAAAGATCCC‑aaaaa                                                                                                             >  2:3640011/1‑149 (MQ=255)
                                            ccGTAACCTCATAACCGCCGCGCTGTGCGTTGTTCAGAATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTAAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGa                                                                                                          <  1:1624100/149‑1 (MQ=255)
                                             cgtaaCCGAATAACCGCCGCGGGGTGCGTTTTTCAGAATCTGCTTTGTCGATACCACGTAATTTTTAATATCCTGTCGTTTGTTTTCTTTTTAAAAATAT‑AATTAATTTTTTTTTTTTTAAATATCGAGATAACGATCAC‑AAACACGAc                                                                                                         <  1:4195801/146‑1 (MQ=255)
                                             cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGAc                                                                                                         <  2:1303781/149‑1 (MQ=255)
                                               gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAATAT‑AATGAAATTATTTTTTTTTTTATATAGAGGTTACGATAAA‑AAAAAAGACaa                                                                                                       >  1:2932719/1‑149 (MQ=255)
                                               gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGGGTGTTAGATTTGAAAATAT‑AATGTAATTATTTTTTTTTTAATATGGTGATAACGTTGAC‑AAAAACGACaa                                                                                                       >  1:3479040/1‑149 (MQ=255)
                                               gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGGGGTTTGGGGTTAAAAAAAA‑AATTTAATTATTTTTTTTTTAATTACGTGAGAACCAAAAC‑CAAAAcaacaa                                                                                                       >  1:2738855/1‑149 (MQ=255)
                                                       tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTGGTCGATACCACGTACATTTTCATTTCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGaaaa                                                                                               <  2:2917011/149‑1 (MQ=255)
                                                          ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATAT‑AATGAAAATATTTTTTTTTAAATATAGAGATAAAGATAAC‑AAAAACGAAAATATGAAAatta                                                                                            >  2:703309/1‑149 (MQ=255)
                                                             ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGGGTTTTTGTTTTGAAAAAATAAATAAAATAATTTTTTTTTAAA‑ATATAGATAAATATAAA‑AAAAAAAAAAAAAAGAAAaatatta                                                                                         >  1:3923725/1‑148 (MQ=255)
                                                              cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATAT‑AATGAAATTATTTTTTTTTAAATATCGAGATAAAGATAAC‑AAAAAAGAAAATATGAAAattattag                                                                                        >  2:1074007/1‑147 (MQ=255)
                                                                 gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGTTTTCAAAATAT‑AATGAAATTATTTTTTTTTTAATATCGTGATAACGATAAC‑AAAAACGACAAAAGTAAAAGTATTAgagg                                                                                     >  2:4314238/1‑149 (MQ=255)
                                                                              ctgcttCTGTTTTGTCTATACCACTTAAATTTTAATATCCTGTGTTTTTTTTTCTATTTAAAAATAT‑ATTTAAATTATTTGTTTTTAAATATCGATATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCaaa                                                                       <  1:2509058/144‑1 (MQ=255)
                                                                                 cttgtGCTTTGTCTAACCCACGTAATTTTTCATTTCCTGTGGTTTTTTTTCTATTTCAAAATAT‑ATTCAATTTTTTTTTTTTTAAATATCTATATACCTAAAAA‑AAACACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCaaaaaa                                                                    <  1:1264095/145‑1 (MQ=255)
                                                                                 attgtgttttGTCTATCCCACTTACATTTTCATATCGTGTGTTTTTTTTTCTATTTAAAAATAT‑AATGAATTTATTTTTTTTTAAATATCTATATAACTATCAA‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCaaaaaa                                                                    <  1:3918291/142‑1 (MQ=255)
                                                                                  atcttttttGTCGATACCAATTAATTTTTCATATCCTGTGTTTTTTTTTCTATTAAAAAATAT‑AATTAAATTTTTTTTTTTTAAATATCGATATAACTAACAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCaaaaaaa                                                                   <  1:1135069/143‑1 (MQ=255)
                                                                                  aTCTGTTTTGTCGACACCACTTAATTTTTCATCTCCTGTTTTTTGTTTTATTTTACAAAATAT‑AATTATATTTTTTTTTTTTAAATATCTATTAAACGAACAC‑AAACACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCaaaaaaa                                                                   <  1:3361913/149‑1 (MQ=255)
                                                                                   tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATAT‑AATGAAATTATTTTTTTTTAAATATAGAGGTAAAGATCAC‑AAAAAAGACAATATTAAAATTATTAGAGGAGT‑GAAAGGCCTAAAAAc                                                                  >  2:3251891/1‑149 (MQ=255)
                                                                                        tttGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGTATTAAAAATAT‑AAATAAAATATTTTTTTTTATATATAGATATAAACAAAAA‑AAAAAAAAAAAAATTAAAAATAATAGTGGAGT‑GTAATGCaaaaaaaatgcca                                                             >  2:776357/1‑143 (MQ=255)
                                                                                        tttGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTTAAAATAA‑AATTAAATTTTTTTTTTTTTTATATAGAGATAACGATCACAAAAAAAGAAAAAAT‑AAAAATATTAGAGGAGTGGAAGGGCAAAAAAACgcca                                                              >  2:776183/1‑146 (MQ=255)
                                                                                         ttGTCGATACCACGAACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATTAAATTATTTTTTTTTAAATATCGCTATAACGATCAC‑AAACACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTc                                                            <  2:1374092/149‑1 (MQ=255)
                                                                                           gTCTATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATAT‑AATTAAATTATTTTTTTTTAAATATCTAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTccc                                                          <  2:651762/149‑1 (MQ=255)
                                                                                           gTCGATACCACTTAAATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑ATTGAAATTATTTGTTTTTAAATATCGAGATACCGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTccc                                                          <  2:651819/149‑1 (MQ=255)
                                                                                              gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTTTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGAt                                                       <  2:3254494/149‑1 (MQ=255)
                                                                                              gATACCACGTAAATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGAt                                                       <  2:3254596/149‑1 (MQ=255)
                                                                                                tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATAT‑AATGAAATTATTTTTTTTTAAATATCGAGATAACCATAAC‑AAAAACGACAAAATGAAAATTATTCTAGGAGT‑GAAAGGCAAAAAAAAGGCCTCCCGagag                                                     >  2:2030462/1‑149 (MQ=255)
                                                                                                tACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAAAAT‑AAATAAATTATTTTTTTTTTAATATCGTGCTAAAGATAAC‑AAAAACCACAAAATGAAAATAATTAGAGGCGT‑GTAAGGCAATAAAAAAGCCAcccccgag                                                     >  2:1863939/1‑144 (MQ=255)
                                                                                                  ccACGTACATTTTCATATCCTGTCGTGTTTTTTAGTTTTGAAAATAT‑AATGAAATTATTTTTTTTTTAATATCGAGATTAAGATAAC‑AAAAAAGCCCAAATGAAACTTATTGGAGGGGT‑GGGAGGCACCAAAAAGGCCAcccccggggg                                                   >  2:3839303/1‑142 (MQ=255)
                                                                                                    aCGTACATTTTCATATCCTGTCGTGTGTTTTCGGTTTGAAAATAT‑AATGAAATTATTTTTTTTTTAATATAGAGATAAAGATCAC‑AAAAAAGACAAAATTGAAATTATTAGAGGGGT‑GAAAGGCCCAAATAAGGCCACCCCAGAGGGaa                                                 >  2:2882500/1‑149 (MQ=255)
                                                                                                    aCGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAAAAT‑AATGTAAATATTTTTTTTTAAAAATAGAGATAAAGATAAC‑AAAAAAGACAAAAATAAAATTATTAGAGGAGT‑GAAAGGCAAAAAAAAGGCCGCCCCAGAGGGaa                                                 >  2:680852/1‑149 (MQ=255)
                                                                                                     cgtaatttttCATCTCCTGTGTTTTGTTTTCGATTTCAAAATAT‑AATTAATTTTTTTTTTTTTAATTATCGATATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                <  1:1788648/143‑1 (MQ=255)
                                                                                                      gttctttttAATATCGTTTCGTTTTTTTTAGATTAAAAAATAA‑AATTAAATTATTTTTTTTTAAATATCTTGATAACTATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                               <  2:1639348/144‑1 (MQ=255)
                                                                                                      gTACTTTTTCATCGCGTGTCGTTTGTTTTCGATTTCAAAATAT‑ACTGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAACACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                               <  2:2415618/149‑1 (MQ=255)
                                                                                                      gTACATTTTCATATCCTGTCGTTTTTTTTCTATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACTATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                               <  2:723514/149‑1 (MQ=255)
                                                                                                       tACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCTATATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                              <  2:1673293/149‑1 (MQ=255)
                                                                                                       tACATGTTCCTCCCCGGTCGTTTGTTTGCGATTTCAAAATCT‑ACTGACATTATTTGTTTTTAACTATCGAGATACCGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                              <  2:1673506/149‑1 (MQ=255)
                                                                                                         cATTTTCATATCCGGTCGTTTGTTTTCTATTACAAAATAT‑AATGAATTTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGt                                            <  2:3550617/149‑1 (MQ=255)
                                                                                                               cATATCCGGTGGTTTGTTTTCGATTTCAAAATAT‑AATTAATTTATTTTTTTTTAATTATCGAGTTAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                      <  2:2642042/149‑1 (MQ=255)
                                                                                                                  agccTGTCTTTTGTTTTCGATTTCAAAATAT‑AATTAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                                   <  2:1258490/147‑1 (MQ=255)
                                                                                                                          gtttgtttTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                           >  2:313867/1‑149 (MQ=255)
                                                                                                                           tttgtttTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                          >  2:3470425/1‑149 (MQ=255)
                                                                                                                                     aTTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa                >  1:2445489/1‑149 (MQ=255)
                                                                                                                                         cAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg            >  2:75019/1‑149 (MQ=255)
                                                                                                                                         cAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg            >  1:4424526/1‑149 (MQ=255)
                                                                                                                                          aaaaTAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg           >  2:3888781/1‑149 (MQ=255)
                                                                                                                                          aaaaTAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg           >  2:3888704/1‑149 (MQ=255)
                                                                                                                                             atat‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt        >  2:2359320/1‑149 (MQ=255)
                                                                                                                                              tat‑aAGTAATTTTTTTTTTTTTAAATATCGAGATAACGAACAC‑AAACACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa       <  1:1996270/149‑1 (MQ=255)
                                                                                                                                               taaaaTCAATTTTTTTTTTTTAAAATATCGATATAACGAACAC‑AAACACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa       <  1:3387555/146‑1 (MQ=255)
                                                                                                                                                    tGAAATTATTTGTTTTTAAATATCTAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt  <  2:1349444/149‑1 (MQ=255)
                                                                                                                                                    tGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc    >  2:1050620/1‑147 (MQ=255)
                                                                                                                                                    tGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAAc    <  1:1050620/147‑1 (MQ=255)
                                                                                                                                                    tGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAt  <  2:4174942/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACAT  >  CP009273/2170576‑2170871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A