Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F86 I0 R1 593 297.4 9515568 94.4% 8982696 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,713 C→A 30.3% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7130CA30.3% 171.7 / 19.1 104intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (16/48);  new base A (23/9);  total (47/57)
Fisher's exact test for biased strand distribution p-value = 1.80e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

TGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  CP009273/2170566‑2170862
                                                                                                                                                    |                                                                                                                                                      
tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTT‑Caa                                                                                                                                                      <  2:1327074/150‑1 (MQ=255)
 ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑Caaa                                                                                                                                                     <  2:3508162/150‑1 (MQ=255)
     gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAatat                                                                                                                                                 <  2:1770711/150‑1 (MQ=255)
      cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAAtata                                                                                                                                                <  2:664397/150‑1 (MQ=255)
      cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAAtata                                                                                                                                                <  1:3339807/150‑1 (MQ=255)
       ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTT‑CAAAATATaa                                                                                                                                               >  2:3351698/1‑150 (MQ=255)
       ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATaa                                                                                                                                               <  2:2538735/150‑1 (MQ=255)
        gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGCTTT‑CAAAATATAAt                                                                                                                                              >  2:2037505/1‑150 (MQ=255)
        gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTT‑CAAAATATAAt                                                                                                                                              >  2:4422906/1‑150 (MQ=255)
         tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑g                                                                                                                                            <  2:4241735/150‑1 (MQ=255)
         tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑g                                                                                                                                            <  1:4119311/150‑1 (MQ=255)
         tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑g                                                                                                                                            <  1:2797883/150‑1 (MQ=255)
          ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑Ga                                                                                                                                           <  1:4276180/150‑1 (MQ=255)
          ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑Ga                                                                                                                                           <  1:4276187/150‑1 (MQ=255)
          ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTTGATTT‑AAAAATATAAtta                                                                                                                                            >  1:1665897/1‑148 (MQ=255)
           tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑Gaa                                                                                                                                          <  1:129485/150‑1 (MQ=255)
           tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑Gaa                                                                                                                                          <  1:2285329/150‑1 (MQ=255)
           tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑Gaa                                                                                                                                          <  1:2285113/150‑1 (MQ=255)
           tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑Gaa                                                                                                                                          <  1:129511/150‑1 (MQ=255)
            cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑Gaaa                                                                                                                                         <  1:3069425/150‑1 (MQ=255)
             tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGTTTT‑AAAAAAATaaataaaa                                                                                                                                         >  1:3319112/1‑144 (MQ=255)
              accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGGTTT‑TAAAAAATaaattaaat                                                                                                                                        >  1:2036536/1‑143 (MQ=255)
              accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTCGGTTT‑GAAAATATAAtttgaat                                                                                                                                        >  1:3909142/1‑144 (MQ=255)
                  ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTAttt                                                                                                                                   <  1:2901812/150‑1 (MQ=255)
                    acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGt                                                                                                                                 <  2:2630223/150‑1 (MQ=255)
                    acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGt                                                                                                                                 <  2:2631416/150‑1 (MQ=255)
                      ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGttt                                                                                                                               <  1:2733062/150‑1 (MQ=255)
                       tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGtttt                                                                                                                              <  2:224996/150‑1 (MQ=255)
                       tGCATCGTTTCGAGATTGAGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGtttt                                                                                                                              <  2:224816/150‑1 (MQ=255)
                        gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑AAAAATATAAT‑GTAAATATtttttttt                                                                                                                             >  2:3412478/1‑150 (MQ=255)
                        gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTT‑AAAAATATAAT‑TAAAATATtttttttt                                                                                                                             >  2:2191463/1‑150 (MQ=255)
                          aTCGTTTCGAGATTGTTAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTaa                                                                                                                           <  2:3556465/150‑1 (MQ=255)
                            cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTTATTT‑AAAAATATTAA‑TAAAtttttttttttttttt                                                                                                                         >  1:4191188/1‑146 (MQ=255)
                             gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAata                                                                                                                        <  2:458690/150‑1 (MQ=255)
                             gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGACGTTTGTTTTAGATAT‑ATAAATATAAT‑GTAAATATATTTTTTTAAata                                                                                                                        >  1:2013441/1‑150 (MQ=255)
                                 cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGATTTAAAAAAAAAAATAAAAATTTTTTTTTTTTatatata                                                                                                                      >  1:605481/1‑149 (MQ=255)
                                 cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGGTTT‑AAAAAAAAAAA‑TAAAATATTTTTTTTTtaaaaaata                                                                                                                    >  1:605192/1‑144 (MQ=255)
                                 cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGTATTT‑AAAAAAATAAT‑AAAATTATTTTTTTTTTAaaatata                                                                                                                    >  1:605271/1‑147 (MQ=255)
                                   agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCgaga                                                                                                                  <  1:376262/150‑1 (MQ=255)
                                   agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCgaga                                                                                                                  <  1:3252264/150‑1 (MQ=255)
                                      ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTT‑AAAAATATAAT‑GAAATTATTTGTTTTTTAATATAGAGATaa                                                                                                               >  2:2360282/1‑150 (MQ=255)
                                       tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                              <  2:1445450/150‑1 (MQ=255)
                                       tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                              <  2:3836316/150‑1 (MQ=255)
                                            atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                         <  1:3013024/150‑1 (MQ=255)
                                             tatTGACTGCCGGAACCTCATAACCGCCGCTCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACTTAAATTTTCATATCCTGTCTTTTGTTTTAGATTT‑AAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                        <  1:2123355/150‑1 (MQ=255)
                                              atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                       <  2:857156/150‑1 (MQ=255)
                                              atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATcacc                                                                                                       >  2:3131013/1‑149 (MQ=255)
                                              atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTT‑AAAAATATAAT‑TAAAATATTTTTTTTTTAATATAGAGATAAAGAtaaaa                                                                                                       >  2:3349841/1‑146 (MQ=255)
                                              atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGTTTT‑CAAAAAATAAT‑TAAAATATTTTTTTTTTTATATAGAGGTTACGATaaca                                                                                                       >  2:3989950/1‑150 (MQ=255)
                                              atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATGTTCATATCCTGTCGTTTGTGTTAGATTT‑GAAAAAATAAA‑TAAATAAATTGTTTTTAAATATAGAGATGActaaaaaa                                                                                                       >  2:3349832/1‑143 (MQ=255)
                                               ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                      <  2:4040585/150‑1 (MQ=255)
                                               ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                      <  1:2724442/150‑1 (MQ=255)
                                               ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTT‑AAAAATATAAT‑GAAATTATTTTTTTTTATATTTCGAGATAACGGTCTCaa                                                                                                      >  2:2984818/1‑150 (MQ=255)
                                                 gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGTTTTT‑AAAAATAAAAA‑TAAAATATTTTTTTTTTTATATATAGATAAACATCaaaaaa                                                                                                    >  1:1319328/1‑150 (MQ=255)
                                                 gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTT‑AAAAATATAAA‑GAAAATATTTTTTTTTAAATATATAGATTACGATCACaaaa                                                                                                    >  1:2997265/1‑150 (MQ=255)
                                                 gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTT‑CAAAAAAAAAT‑TAAATTATTTTTTTTTTTATATAGAGGGAAAGATCACaaaa                                                                                                    >  1:627522/1‑150 (MQ=255)
                                                   aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                  <  2:849488/150‑1 (MQ=255)
                                                   aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTT‑AAAAAAATAAT‑TAAAATATTTTTTTTTAAAAATAGAGATAAAAAACAaaaaaaa                                                                                                  >  2:3676275/1‑149 (MQ=255)
                                                   aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTT‑TAAAATATAAT‑TAAAATATTTGTTTTTAAATATAGAGATAACCAACACAAAAAc                                                                                                  >  2:3676725/1‑150 (MQ=255)
                                                       cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTT‑AAAAATATAAT‑GAAAATATTTTTTTTTTAATATAGAGATAACGATCACAAAAACGACa                                                                                              >  2:1729816/1‑150 (MQ=255)
                                                        ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTT‑AAAAATATAAT‑GAAATTATTTGTTTTTTAATATCGAGATAACGATCACAAAAACGACaa                                                                                             >  2:2692681/1‑150 (MQ=255)
                                                          aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTGTTCGAGTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAata                                                                                           >  2:3699414/1‑150 (MQ=255)
                                                            ccGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTT‑CAAAATATAAT‑TAAATTATTTTTTTTTTAATATAGAGATAAAGATAACAAAAACGACAATATg                                                                                         >  2:2348259/1‑150 (MQ=255)
                                                            ccGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTT‑GAAAAAAAAAT‑TAAAATATTTTTTTTTTTAAATAGATATTAAGATCACAAAAAAGACaaaaag                                                                                         >  1:653696/1‑146 (MQ=255)
                                                              gaaTAACCGCCGCGCTGTGCTTTGTTCAGCATATGATTTGTCGATACAACTTACATTTTCATATCCTGTCTTTTTTTTTCTATTT‑CAAAATATAAT‑TAAATTATTTGTTTTTAAATATCGATATAACTATCACAAAAACGACAATATGaa                                                                                       <  2:1404571/148‑1 (MQ=255)
                                                                 tAACCGCCGCGCTGTGCTTTGTTCAGCATCTTCTTTGTCTATACCACTTACTTTTTAATATCCTGTCTTTTTTTTTCTATTT‑AAAAATATAAT‑GAAATTTTTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAat                                                                                    <  1:1160104/150‑1 (MQ=255)
                                                                   cccgccgCGGTGTGCGTTGTTCAGCATCTGCTTTGTCTATACCACTTAAATTTTAATATCCTGTGGTTTGTTTTCTATTT‑AAAAATATAAT‑GAATTTATTTTTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAatta                                                                                  <  2:442312/149‑1 (MQ=255)
                                                                   cccgccgCGCTGTGCGTTGTTCCGAATCTGCTTTGTCTATACCACTTAAATTTTCATATCCTGTCGTTTGTTTTCTATTA‑CAAAATATAAT‑GAATTTATTTTTTTTTAAATATGGAGATAACTAACACAAACACGACAATATGAAAatta                                                                                  <  2:442359/149‑1 (MQ=255)
                                                                     cgccgcGCTGTGCGTTTTTCAGCTTCTGCTTTGTCGATACCACGTACATTTTAATATCGTGTCTTTTGTTTTCTATTA‑CAAAATTTAAT‑GAAATTATTTGTTTTTAAATATCGATATAACCATCACAAACACGACAATATGAAAattatt                                                                                <  2:2452934/150‑1 (MQ=255)
                                                                           gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                          <  2:2715472/150‑1 (MQ=255)
                                                                             tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTT‑AAAAAAAAATT‑TTAATTATTTGTTTTTTAATATCGAGTTAACCATCTCAAAAAAGCCAAAAAGAAAATTATAAGAGGGGt                                                                        >  2:829631/1‑150 (MQ=255)
                                                                                 cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTGGTTT‑AAAAATAAAAT‑TAAAATATTTTTTTTTTAATATATTTATAAAAATAACAAAAAAAACAAAAATAAAATTATTAGTGGAGTGtaa                                                                    >  1:1736570/1‑150 (MQ=255)
                                                                                        cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTT‑GAAAATAAAAT‑TAAAATATTTTTTTTTAAATATAGAGAAAAACAAAACAAAAAACACCAAATGAAAAAAATTATAGGAGTGGAACGCaaaa                                                             >  1:2696485/1‑150 (MQ=255)
                                                                                           cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTT‑TAAAAAAAAAT‑TAAAATAATTTTTTTTAAATAAAGAGATAAACAAAACAAAAAACACAAAATTAAAAATATTCTATGAGTGAAAGGCcaaaaaa                                                          >  1:3161467/1‑150 (MQ=255)
                                                                                            ttCTGCTTTGTCTATACCACTTACTTTTTCATATCCTGTCGTTTTTTTTCGATTT‑CAAATTATATT‑GAATTTATTTGTTTTTAAATATCGATATAACGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  2:61746/149‑1 (MQ=255)
                                                                                            aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTTTTT‑CAAAATATAAT‑GAATTTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  1:771234/150‑1 (MQ=255)
                                                                                            aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTT‑CAAAATATAAT‑CAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  2:4222364/150‑1 (MQ=255)
                                                                                            aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTTATTT‑AAAAAAATAAA‑AAAAATATTTTTTTTTAAAAATATAGTTAAAGAAAAAAAAAAAAAAAAAATTAAAAATATTCGAGGAGTGAAATGCaaaaaaaa                                                         >  2:1637045/1‑149 (MQ=255)
                                                                                            aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTT‑AAAAATATAAA‑TTAAATATTTTTTTTTAAAATACGAGATAAACAAAACAAAAAACAAAAAAATAAAAATAATATTGGCGTGAAAGGCcaaaaaaa                                                         >  2:1637026/1‑149 (MQ=255)
                                                                                            aTCTGCTTTGTCGATACCACATACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  2:381923/150‑1 (MQ=255)
                                                                                             tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGCTTT‑AAAAATATAAA‑TAAAATATTTTTTTTTTAAAATACTTATAAAAAAAACAAAAAAAACAAAAATAAAAAAATTAGAGGAGTGTAAGGCaaaaaaaac                                                        >  2:459750/1‑148 (MQ=255)
                                                                                              cTGCTTTGTCCAAACAACTAACATTTTAATATCCTTTCTTTTTTTTTCGATTA‑AAAAATAAAAA‑GAAATTATTTTTTTTAAAATATCGAGAAAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                       <  2:478498/150‑1 (MQ=255)
                                                                                              cTGATTTTTCTATACCAATTAAATTTACATATCCTTTCGTTTTTTTTCTATTT‑AAAAATATAAT‑TAATTTATTTGTTTTTAAATATCTAGAAAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACgg                                                       <  2:478563/150‑1 (MQ=255)
                                                                                                      tCGAAACCACGTAAATTTTCATATCCTGTCGTTTTTTTTCTATTT‑CAAAATATAAT‑CAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                               <  2:3842212/150‑1 (MQ=255)
                                                                                                              ccttACTTTTTCATTGCGTGTGGTTTTTTTTCTATTA‑AAAAATATAAT‑GAATTTTTTTTTTTTTAAATATCGAGATACCGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                       <  2:825411/147‑1 (MQ=255)
                                                                                                                  aCATTTTCATATCCTGTCGTTTGTTTTGGATTT‑AAAAAAAAAAT‑TAAAATATTTTTTTTTTAATATAGAGATAAAGATAACAAAAACGCAAATATGAAAATTATTCGAGGCGTGAAAGGCCAAAAAAAGGCCCCCCGCTTGGGCAGccct                                   >  2:3038998/1‑148 (MQ=255)
                                                                                                                  aCATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑CAAATTATTTGTTTTTAAATATCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGt                                   <  2:201144/150‑1 (MQ=255)
                                                                                                                  aCATTTTCATATCCTGTCGTGTTTTTTCGATTT‑CAAAATATAAT‑GAAAATATTTTTTTTTAAATATAGAGATAACCATCACAAAAAACCAAATATGAAAATTATTCGCGGAGTGTAAGGCAAAAAAACGGCCAACCCGGAGGGCAGCCGt                                   >  2:3038974/1‑150 (MQ=255)
                                                                                                                   atttttAATATCCTGTCGTTTTTTTTCTATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  2:3350215/148‑1 (MQ=255)
                                                                                                                       ttCATATCGTTTTTTTTGTTTTCTTTTT‑AAAAATATAAT‑TAATTTTTTTTTTTTTAAATATCTATATAACTAACAAAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaa                              <  2:3585454/150‑1 (MQ=255)
                                                                                                                             tCCTGTCGTTTTTTTTAGATTT‑CAAAAAATAAT‑GAAATTATTTTTTTTTAAATATCGAGAAAACGAAAACAAAAAAGACAAAATTTAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCACCCGATAGGGAAGCCCTAGCAAAATgcg                        >  2:701024/1‑150 (MQ=255)
                                                                                                                                tgtgtttttttttCTATTT‑AAAAATATATT‑TAATTTTTTTTTTTTTAATTTTCTATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     <  2:1203182/145‑1 (MQ=255)
                                                                                                                                tGTCGTTTTTTTTCTATTT‑AAAAATATAAT‑TAAATTATTTTTTTTTAAATATCGAGATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     <  2:3598053/150‑1 (MQ=255)
                                                                                                                                    gtttgtttTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCAAAGTGCGCGTGttt                 >  2:4018226/1‑150 (MQ=255)
                                                                                                                                    gtttgtttTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                 >  1:4137930/1‑150 (MQ=255)
                                                                                                                                     tttgtttTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                >  2:4647953/1‑150 (MQ=255)
                                                                                                                                         ttttCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            >  1:2211302/1‑150 (MQ=255)
                                                                                                                                               aTTT‑CAAAATTTAAT‑GAAATTTTTTGTTTTTAAATATCGAGATAACGTTCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt      <  2:1732940/150‑1 (MQ=255)
                                                                                                                                                ttt‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg     >  1:4538798/1‑150 (MQ=255)
                                                                                                                                                    cAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  2:4243752/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTT‑CAAAATATAAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  CP009273/2170566‑2170862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A