Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,713 C→A 24.6% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7130CA24.6% 313.8 / 27.7 179intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (35/84);  new base A (19/25);  total (70/109)
Fisher's exact test for biased strand distribution p-value = 1.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 181 total aligned reads displayed.

AGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGC  >  CP009273/2170570‑2170860
                                                                                                                                                 |                                                                                                                                                    
aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑Caaaa                                                                                                                                                  <  1:354241/148‑1 (MQ=255)
 gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAat                                                                                                                                                 <  1:307970/148‑1 (MQ=255)
  cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAata                                                                                                                                                <  2:1463797/148‑1 (MQ=255)
  cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTT‑GTATTT‑Taaaaaa                                                                                                                                                >  1:2503367/1‑146 (MQ=255)
    gTTTCTCCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAAtat‑a                                                                                                                                             <  2:2750314/148‑1 (MQ=255)
        cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGa                                                                                                                                         <  2:1353951/148‑1 (MQ=255)
        cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGa                                                                                                                                         <  2:1798828/148‑1 (MQ=255)
          accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTT‑CGATTT‑CAAAATAT‑AATTaaa                                                                                                                                       >  2:2949094/1‑148 (MQ=255)
          accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CTATTT‑CAAAATAT‑AATGaaa                                                                                                                                       <  2:167331/148‑1 (MQ=255)
          accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CTATTT‑CAAAATAT‑AATGaaa                                                                                                                                       <  2:1838022/148‑1 (MQ=255)
          accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGaaa                                                                                                                                       <  2:1590621/148‑1 (MQ=255)
          accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGaaa                                                                                                                                       <  1:2742981/148‑1 (MQ=255)
          accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTT‑CGATTT‑CAAAATAT‑AATTaaa                                                                                                                                       >  2:2948935/1‑148 (MQ=255)
          accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTT‑AGATTT‑AAAAAAAT‑AAAGaaa                                                                                                                                       >  1:112764/1‑148 (MQ=255)
           ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑GGATTT‑AAAAATAT‑AAtttaat                                                                                                                                      >  2:1141079/1‑143 (MQ=255)
            caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAatt                                                                                                                                     <  1:3159760/148‑1 (MQ=255)
            caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAatt                                                                                                                                     <  2:2613769/148‑1 (MQ=255)
             accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAatta                                                                                                                                    <  1:2510418/148‑1 (MQ=255)
               cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAattatt                                                                                                                                  <  2:2153285/148‑1 (MQ=255)
               cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTT‑CGATTT‑CAAAATAT‑AAATAAattatt                                                                                                                                  >  2:455164/1‑148 (MQ=255)
                 cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTg                                                                                                                                <  1:1532662/148‑1 (MQ=255)
                 cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTg                                                                                                                                <  2:480558/148‑1 (MQ=255)
                  ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTT‑AGATTT‑AAAAAAAT‑AAAGAAAATAttttt                                                                                                                               >  1:1075843/1‑146 (MQ=255)
                   tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTAtttttt                                                                                                                              >  2:501896/1‑148 (MQ=255)
                   tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑AGATTT‑CAAAATAT‑AATTAAATTAtttttt                                                                                                                              >  2:501874/1‑148 (MQ=255)
                        cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTaa                                                                                                                         <  1:695986/148‑1 (MQ=255)
                        cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTaa                                                                                                                         <  2:3140885/148‑1 (MQ=255)
                          tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAat                                                                                                                       <  1:1801865/148‑1 (MQ=255)
                          tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAat                                                                                                                       <  1:771986/148‑1 (MQ=255)
                           ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAata                                                                                                                      <  2:1443990/148‑1 (MQ=255)
                              gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCg                                                                                                                   <  2:1154370/148‑1 (MQ=255)
                               agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTT‑TGATTT‑AAAAAAATAAATAAAATAATTTTTTTTTaatttaaa                                                                                                                   >  1:2301374/1‑142 (MQ=255)
                                gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCgag                                                                                                                 <  2:2413471/148‑1 (MQ=255)
                                gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCgag                                                                                                                 <  1:530562/148‑1 (MQ=255)
                                 aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑GGATTT‑CAAAAAAT‑AATGAAAATATTTTTTTTTTAATATAgaga                                                                                                                >  2:881091/1‑148 (MQ=255)
                                  ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTTTTTTTTAATATGGAGAt                                                                                                               >  2:1969733/1‑148 (MQ=255)
                                      gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTT‑TGATTT‑GAAAATAA‑AAATTAAAAATTTTTTTTTTAATATAGAGATAAcc                                                                                                           >  1:2933885/1‑147 (MQ=255)
                                        atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                         <  1:197771/148‑1 (MQ=255)
                                         tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                        <  2:374173/148‑1 (MQ=255)
                                          atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTTAATATAGAGTTAAAGATca                                                                                                       >  2:2365009/1‑148 (MQ=255)
                                          atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                       <  1:2614460/148‑1 (MQ=255)
                                           ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑GTATTT‑AAAAATAT‑AATGTAATTATTTGTTTTTTAAAATCGAGATAACCATcaa                                                                                                      >  2:1057893/1‑147 (MQ=255)
                                             gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                    <  1:3293498/148‑1 (MQ=255)
                                              aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑GGTTTT‑GAAAATAT‑AATGAAAATATTTTTTTTTTAATATCGAGATAACGATCACaaa                                                                                                   >  2:1037559/1‑148 (MQ=255)
                                              aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAAATATTTTTTTTTTAATATAGAGATAAAGATCACaaa                                                                                                   >  2:1037527/1‑148 (MQ=255)
                                               aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                                  <  1:487979/148‑1 (MQ=255)
                                                tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                 <  1:1127072/148‑1 (MQ=255)
                                                    ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                             <  2:3268791/148‑1 (MQ=255)
                                                     gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTT‑CGATTT‑GAAAATAT‑AATGTAATTATTTGTTTTTTAATATAGAGATAAAGATCACAAAAACGACa                                                                                            >  2:1102619/1‑148 (MQ=255)
                                                             tAACCGCCGCGCTGTGCTTTTTTCAGCATCTGCTTTGTCGATACCACGTAAATTTTAATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGaaa                                                                                    <  2:2354771/148‑1 (MQ=255)
                                                             tAACCGCCGCGCTGGGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTGT‑CGATTT‑AAAAATAT‑AATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaa                                                                                    <  2:781283/148‑1 (MQ=255)
                                                              aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACTTACTTTTTCATATCCTGTCGTTTTTTTT‑CGATTT‑CAAAATAT‑AATCAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGaaaa                                                                                   <  2:953093/148‑1 (MQ=255)
                                                              aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTACAATTA‑AAAAAAAA‑AATAAAATAAATT‑TTTTTAAATAAAGACATAAAGTTAAAAAAAAAAAAAatatttaaa                                                                                   >  1:1397446/1‑143 (MQ=255)
                                                                 cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CTATTT‑CAAAATAT‑AATTAAATTATTTGTTTTTAAATATCGATATAACTATCACAAAAACGACAATATGAAAatta                                                                                <  2:423675/148‑1 (MQ=255)
                                                                 cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatta                                                                                <  2:3017914/148‑1 (MQ=255)
                                                                       gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑AGATTT‑CAAAATAT‑AAATAAATTATTTTTTTTTAAATAAAGAGATAACGATCAAAAAAAACACAAAATTAAAAATATTagag                                                                          >  2:3222796/1‑148 (MQ=255)
                                                                          gtgGGTTGTTCCGAATATTCTTTTTCTATACCACGTAAATTTTCATATCTTGTGTTTTTTTTT‑CGATTT‑AAAAATAT‑AATTAAATTTTTTTTTTTTAAATATCGAGTTAACTATCACAAAAACGACAATATGAAAATTATTCgaggag                                                                       <  2:1068356/148‑1 (MQ=255)
                                                                               ttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTT‑GGATTT‑AAAAAAAA‑AAATAAAATAATTTTTTTTAAATATAGAGATAAAGATCACAAAAACGACAAAAAGAAAAATATTGGAGGAGTGcaa                                                                  >  2:1237016/1‑148 (MQ=255)
                                                                                 gtccaccATCTGCTTTGTCGATCCCACTTAAATTTTCTTCTCCTGACTTTTGTTTT‑CGATTA‑CAAAATAT‑AATCAAATTTTTTTTTTTTAAATATCGATATAACGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                <  1:689159/142‑1 (MQ=255)
                                                                                  ttCAGAATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTTTTTTAAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGc                                                               <  2:3304885/148‑1 (MQ=255)
                                                                                  tacAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGc                                                               <  2:2183411/146‑1 (MQ=255)
                                                                                   tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAAATATTTTTTTTTAAATATAGAGATAACGATAACAAAAACGACAATATGTAAAATATTAGAGGTGGGaaatggc                                                              >  2:2719528/1‑144 (MQ=255)
                                                                                    cAGCATCTTCTTTGTCCACACCACGTATATTTTAATATCCTGTGTTTTTTTTT‑CTATTA‑AAAAATAT‑AATAAATTTTTTTTTTTTTAAATATCGATATCACGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaa                                                             <  1:3056808/148‑1 (MQ=255)
                                                                                     cgCATCTGCTTTGTCGATACCACTTACATTTACATACCCTGTCTTTTTTTTT‑CTTTTT‑AAAAATAT‑AATTAATTTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                            <  1:1904457/147‑1 (MQ=255)
                                                                                     agaaTCTGTTTTGTCTAAACCACTTACATTTTAATATCCTTTCTTTTGTTTT‑ATATTA‑AAAAATAT‑AATTAAATTATTTTTTTTTAAATATCGACAAAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                            <  1:2540428/145‑1 (MQ=255)
                                                                                     agaaTCTGCTTTGTCTATCCCAGCTACTTTTTAATATCCTGTGTTTTTTTTT‑TGTTTA‑AAAATTAT‑ATTCAATTTTTTTTTTTTTAAATATCGAGAAAACGTTCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                            <  1:885358/145‑1 (MQ=255)
                                                                                        aTCTGCTTTGTCGAGACCACGTACATTTACATATCCTGTCTTTTGTTTT‑CTTTTA‑CAAAATAT‑AATCAATTTTTTTGTTTTTAAATATCGATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaa                                                         <  1:417909/148‑1 (MQ=255)
                                                                                         tattattttTCTATACAACCTAAATTTTAATATCCTGTGTTTTGTTTT‑ATATTT‑AAAAATAT‑AATTAAATTTTTTTTTTTAAAATATCTATAAAACTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                        <  1:1188866/143‑1 (MQ=255)
                                                                                          cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑GGATTT‑GAAAATAT‑AATTAAATTTTTTTTTTTTAAATATAGAGATAAAGATAACAAAAACTACAAAATTTAAATTTTTAGAGGGGTGAAATGGaaaaaaaa                                                       >  2:2859694/1‑147 (MQ=255)
                                                                                          cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTT‑TGATTT‑AAAAAAAA‑AATTAAAATATATTTTTTTTAATATAGAGATAAACATAAAAAAAAAGACAAAATAAAAAATTTTGGAGGAGGGGAATGCaaaaaaaa                                                       >  2:1977942/1‑147 (MQ=255)
                                                                                             cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTT‑AGATTTGAAAAAAAA‑AATTAAATAATTT‑TTTTTTTATATAGATATAAAGATGACAAAAAAGAACAAATGTAAAATAATTGAGGCGTGTATAGCaaaaaaaaagg                                                    >  1:3229847/1‑144 (MQ=255)
                                                                                             cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑AGTTTT‑GAAAATAT‑AATTAAATTATTTTTTTTTTAAATTAGAGTTAAAGATCACAAAAAAGACAATATGTAAATTTTTGGGGGTGGGTAAGGCAAAAAAACGGc                                                    >  2:3140523/1‑148 (MQ=255)
                                                                                             cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTT‑AGTTTT‑AAAAATAA‑AATGAAATTATTTTTTTTTATATATAGAGATAAAAATAACAAAAACGAAAATAAGAAAATTATAAGAGGAGTGAAAGGCaacaaaaagtc                                                    >  2:3139917/1‑144 (MQ=255)
                                                                                                   cGATACCACGTACATTTTCATATCCTGTCGTGTGTTTT‑GGATTT‑CAAAATAT‑AATTTAAATATTTTTTTTTATATATAGTGATAAAGAACACAAAAAAGACAAAATTTAAATTATTTGAGGTGGGTAAGGGCAAAAAACTGCCTGCCg                                              >  2:2005964/1‑148 (MQ=255)
                                                                                                            gTACATTTTCTTATCCTGTCTTTTGTTTT‑CTATTT‑AAAATTAT‑AATGAATTTATTTTTTTTTAAATATCGATATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                     <  2:1257701/148‑1 (MQ=255)
                                                                                                              aCATTTTCATATCCTGTCGTGTGTTTT‑CGATTT‑CAAAATAT‑AATGTAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACCACACAAAGAAAAATATTCGAGGGGTGGAAGGCCAAAAAACGGCCTCCCGCGAgggcacac                                   >  2:2959608/1‑143 (MQ=255)
                                                                                                                  tttCATATCCTGTCGTTTGTTTT‑CTATTT‑AAAAATAT‑AATGAAATTATTTTTTTTTAAATATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                               <  2:2920657/148‑1 (MQ=255)
                                                                                                                  tttCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                               <  2:1271837/148‑1 (MQ=255)
                                                                                                                  tttCATATCCTGTCGTGTGTTTT‑AGATTT‑CAAAAAAA‑AATGTAAATATTTGTTTTTAAATATCGAGATAAAGCAAACAAAAACGACAATAAGCAAATTATTGGTGGGGTGCAAGGCCAAAAAAACGCCTCCCGCTAGGGCACCCGGAg                               >  2:3198877/1‑148 (MQ=255)
                                                                                                                     cagatCCTGTCGTTTGTTTT‑CTTTTT‑CAAAATAT‑AATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaa                            <  2:1222041/145‑1 (MQ=255)
                                                                                                                     cATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaa                            <  2:1782950/148‑1 (MQ=255)
                                                                                                                         gcgtGTCGTTTGTTTT‑CTTTTA‑AAAAATAT‑AATTAAATTATTTTTTTTTAAATATCGAGAAAACCATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                        <  1:501896/145‑1 (MQ=255)
                                                                                                                            ggtgttttttttt‑CTATTT‑AAAAATAT‑AATTAATTTATTTGTTTTTAAATATCTATAAAACGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                     <  1:981953/143‑1 (MQ=255)
                                                                                                                             gTCTTTTTTTTA‑AGATTT‑AAAAATAT‑AATGAAATTATTTTTTTTTAAATATCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcg                    <  2:2301374/148‑1 (MQ=255)
                                                                                                                               cttttgtttT‑CTATTA‑CAAAATAT‑AATTAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                  <  1:1663524/146‑1 (MQ=255)
                                                                                                                                gtttttttT‑CTATTT‑CAAAATAT‑AATTAAATTATTTTTTTTTAAATATCGATATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                 <  2:616705/148‑1 (MQ=255)
                                                                                                                                  ttttttt‑CTATTA‑AAAAATAT‑AATCAAATTTTTTTTTTTTAAATAGCGACATACCCAACAAAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt               <  1:677109/145‑1 (MQ=255)
                                                                                                                                  ttgtttT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt               >  1:2381717/1‑148 (MQ=255)
                                                                                                                                  ttgtttT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt               >  2:306142/1‑148 (MQ=255)
                                                                                                                                   tgtttT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt              >  1:1032232/1‑148 (MQ=255)
                                                                                                                                    ttttt‑CTATTA‑AAAAATAT‑AATTAATTTTTTTTTTTTTAAATATCTATATAACTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt             <  1:969156/147‑1 (MQ=255)
                                                                                                                                     tttt‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa            >  1:2279501/1‑148 (MQ=255)
                                                                                                                                      ttt‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt           >  1:1263350/1‑148 (MQ=255)
                                                                                                                                        t‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc         >  2:1083107/1‑148 (MQ=255)
                                                                                                                                           gATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGGTTTTATGCCg       >  2:2191739/1‑148 (MQ=255)
                                                                                                                                            aTTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg      >  1:2071868/1‑148 (MQ=255)
                                                                                                                                             ttt‑CAAAATAT‑AATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa     <  2:2725282/148‑1 (MQ=255)
                                                                                                                                              tt‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt    >  1:1468992/1‑148 (MQ=255)
                                                                                                                                               t‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg   >  1:2678174/1‑148 (MQ=255)
                                                                                                                                                 cAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc  >  1:1853537/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
AGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTT‑CGATTT‑CAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGC  >  CP009273/2170570‑2170860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A