Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,713 C→A 24.8% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7130CA24.8% 302.7 / 16.6 164intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (36/68);  new base A (23/18);  total (78/86)
Fisher's exact test for biased strand distribution p-value = 2.40e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

GCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATG  >  CP009273/2170568‑2170859
                                                                                                                                                 |                                                                                                                                                  
 cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaa                                                                                                                                                 <  1:2358268/148‑1 (MQ=255)
 cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGGTTTGaaa                                                                                                                                                 >  2:780829/1‑148 (MQ=255)
  aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                <  2:460403/148‑1 (MQ=255)
  aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                >  2:866313/1‑148 (MQ=255)
  aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAaaaa                                                                                                                                                >  2:1662770/1‑148 (MQ=255)
    cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGGATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                              <  2:2547483/148‑1 (MQ=255)
    cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAata                                                                                                                                              >  2:2085342/1‑148 (MQ=255)
    cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTGAAAata                                                                                                                                              >  1:1333113/1‑148 (MQ=255)
      gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCTCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                            <  2:3405048/148‑1 (MQ=255)
      gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                            <  1:908768/148‑1 (MQ=255)
      gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                            <  1:1621099/148‑1 (MQ=255)
      gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                            <  1:866761/148‑1 (MQ=255)
      gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGTTTTAAAAAaata                                                                                                                                            >  1:3529552/1‑148 (MQ=255)
      gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTGaaaaaaaa                                                                                                                                            >  1:3529680/1‑144 (MQ=255)
       tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                                                                           <  1:2044584/148‑1 (MQ=255)
       tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                                                                           <  1:3153752/148‑1 (MQ=255)
       tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGATTTGAAAAAATaa                                                                                                                                           >  1:3112486/1‑148 (MQ=255)
         tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATg                                                                                                                                         <  1:769810/148‑1 (MQ=255)
            accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGGTTTCAAAATATAATTaaa                                                                                                                                      >  2:1620216/1‑148 (MQ=255)
            accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGGTTTCGATTTCAAAAAATAATGaaa                                                                                                                                      >  2:1131394/1‑148 (MQ=255)
             ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                     <  1:1948397/148‑1 (MQ=255)
             ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                     <  2:2677420/148‑1 (MQ=255)
                ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                  <  2:3383821/148‑1 (MQ=255)
                 cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                 <  2:3481818/148‑1 (MQ=255)
                  acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                <  1:1136439/148‑1 (MQ=255)
                   cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTTATTTTAAAATATAATTTAAttttttt                                                                                                                               >  1:2548253/1‑147 (MQ=255)
                   cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTATATTTTAAAAAATAAATAAAATAtttt                                                                                                                               >  1:2548259/1‑147 (MQ=255)
                   cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTg                                                                                                                               <  2:1166996/148‑1 (MQ=255)
                      gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                            <  1:2934149/148‑1 (MQ=255)
                      gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                            <  1:1161829/148‑1 (MQ=255)
                        aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttttt                                                                                                                          <  2:903111/148‑1 (MQ=255)
                          cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                        <  1:654861/148‑1 (MQ=255)
                          cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                        <  1:597341/148‑1 (MQ=255)
                           gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaaa                                                                                                                       <  2:478373/148‑1 (MQ=255)
                            tttCGAGATTGTGAATATTGAATGCCGGAACCGCATACCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAat                                                                                                                      <  2:2186112/148‑1 (MQ=255)
                            tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAat                                                                                                                      <  1:3463437/148‑1 (MQ=255)
                             ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAATATAATTGAATTATTTGTTTTtatata                                                                                                                     >  2:3372261/1‑148 (MQ=255)
                                gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                  <  1:1720973/148‑1 (MQ=255)
                                 agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTGAAAAAATAATAAAAATATTTTTTTTTTTatatgtt                                                                                                                 >  1:2989322/1‑145 (MQ=255)
                                   aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTTATTTAAAAATATAATTAAAAAATATTTTTTTTAatataaagt                                                                                                               >  1:118047/1‑143 (MQ=255)
                                     tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATa                                                                                                             >  2:55801/1‑148 (MQ=255)
                                        gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAATTATTTGTTTTTAAATATAGATATAACg                                                                                                          >  2:2269662/1‑148 (MQ=255)
                                         aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                         <  1:1139972/148‑1 (MQ=255)
                                         aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGa                                                                                                         >  2:1141542/1‑148 (MQ=255)
                                         aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAAAATCGAGATAAcca                                                                                                         >  2:2915670/1‑146 (MQ=255)
                                           tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGTTTTGAAAAAATAATTAAATTATTTTTTTTTTTATATAGTGATaaaaaac                                                                                                       >  1:2739125/1‑143 (MQ=255)
                                            atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGGTTTAAAAATATAATTTAATTATTTTTTTTTTAATATAGTGTTTACGAtga                                                                                                      >  1:3518168/1‑146 (MQ=255)
                                            atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTTATTTGAAAATATTATTCAAATATTTTTTTTTTTATATAGTGATATCGCTca                                                                                                      >  1:3395117/1‑148 (MQ=255)
                                                 aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGTTTTCAAAATATAATGAAATTATTTGTTTTTTTTTATAGAGTTAAAGATCACaaaa                                                                                                 >  2:1896682/1‑148 (MQ=255)
                                                 aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                                 <  2:1627818/148‑1 (MQ=255)
                                                  tgtcGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                <  2:2412466/145‑1 (MQ=255)
                                                  tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                <  2:1266673/148‑1 (MQ=255)
                                                   gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTGAAAATATAATTAAAATATTTTTTTTTAAATATCGGGGTAAAGATAACaaaaaa                                                                                               >  1:1485875/1‑147 (MQ=255)
                                                     cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGCTTTGAAAATATAAAGAAAATATTTTTTTTTTAATATAGAGGTAAAGATGACaaaaaaca                                                                                             >  2:2704069/1‑145 (MQ=255)
                                                      ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATAGAGATAAAGATCACaaaaaaaac                                                                                            >  2:437221/1‑144 (MQ=255)
                                                           cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGTAATTATTTTTTTTTAAAAATCGAGATAACGATCACAAAAACGACAatat                                                                                       >  2:138953/1‑148 (MQ=255)
                                                              aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTCGTTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGCTAACGATAACAAAAACGACAATATGaa                                                                                    >  2:1752811/1‑148 (MQ=255)
                                                               tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGTTTTCAAAAAATAATGGAATTATTTTTTTTTAAATATCGTGATAACGCTACGAAAAAAGAAAATATGtaa                                                                                   >  2:1974748/1‑148 (MQ=255)
                                                                       gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCg                                                                           <  2:585984/148‑1 (MQ=255)
                                                                        cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTGAAAATTTAAATAAATTATTTTTTTTTAAATATAGAGTTAAAGATAAAAAAAAACAAAAAATTAAAaatattata                                                                          >  2:430808/1‑145 (MQ=255)
                                                                           tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAATATAAAGAAATTATTTTTTTTTAAAAATCGAGATAAAGATCACAAAAACGACAAAATGAAAattattatatga                                                                       >  2:3461185/1‑142 (MQ=255)
                                                                           tgtgCGTTGTTCAGCATCTGCTTGGTCGATACCACGTACATTTTCTTATCCTGTCTTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                       <  2:3056968/148‑1 (MQ=255)
                                                                           tgtgCGTTGTTCAGCATCGGCTTTGTCGATACCACGTACATTTTCATATCCGGTCGTTTGTTTTCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                       <  2:1545948/148‑1 (MQ=255)
                                                                            gtgGGTTGTTCAGCAACGGCTTTGTCGATACAACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgaggag                                                                      <  2:1256245/148‑1 (MQ=255)
                                                                                  tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAAATAAAATATTTTTTTTTAAATAAAGATATAAAGAAAAAAAAAAAGACAAAATGAAAAATATTCGTGGCGTGTAAg                                                                >  1:2926789/1‑148 (MQ=255)
                                                                                   gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGATTTGAAAAAATAAATAAAATATTTTTTTTTTAATATATAAATAAACATAAAAAAAAAGACAATAATAAAAATATTCTAGGAGTGaaaag                                                               >  1:2323335/1‑146 (MQ=255)
                                                                                    ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTATATTTTAAAATATAATTTAATTATTTTTTTTTTAATATAGAGTTTAAGATAACAAAAAACAAACAAAGAAAAATATTTGAGGTGTGAAATGc                                                              >  1:512108/1‑148 (MQ=255)
                                                                                               cTTTGTCGAAACCACGTAAATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                   <  2:263865/148‑1 (MQ=255)
                                                                                                 ttGTCGAAACAACTTAATTTTAAATATCGTGTCTTTTGTTTTCTTTTTAAAAATATAATTAAATTTTTTTTTTTTAAATATCTATATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCt                                                 <  1:556250/148‑1 (MQ=255)
                                                                                                  tGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTc                                                <  2:1127291/148‑1 (MQ=255)
                                                                                                    tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACCATCACAAAAAACACAATAAGAAAATTATTACAGGGGTGAAAGGCAAAAAAACGGCCTccc                                              >  2:2039195/1‑148 (MQ=255)
                                                                                                     cGATACCACTAACATTTTCAAATCCTGTCGTTTGTTTGCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCg                                             <  2:2026897/148‑1 (MQ=255)
                                                                                                       aaaCCACGTACATTTTAATATCCTGTCTTTTGTTTTCTTTTTCAAAATATAATGAATTTATTTTTTTTTAAATATCGAGTTAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAt                                           <  1:2085439/146‑1 (MQ=255)
                                                                                                       aTACCCGGTACTTTTTCATAGCGTGTGTTTTGTTTTGGTTTTAAAAATATAATGAATTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAt                                           <  1:2915659/148‑1 (MQ=255)
                                                                                                       aTACCACGTACTTTTTCATATCGTGTCTTTTGTTTTCGTTTTAAAATTATAATTAATTTATTTTTTTTTAAATATCGAGTTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAt                                           <  1:2915670/148‑1 (MQ=255)
                                                                                                        tACCACGTACATTTTCATATCCTGTCGTTGGTTTTTGTTTTTAAAATATAATTTAATTATTTTTTTTTTTATTTAGAGATAACGATAAAAAAAAAGAAAAAATGAGAATTATTAGAGGGGGGAAAGGCCAAAAAAAGGCCGcccccga                                          >  2:1327893/1‑144 (MQ=255)
                                                                                                          ccACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTGAAAAAATAATGTAATTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAAAAACAATATGTAAATTATTTGGGGGGTGCGAGGCACAAAAAAGGCCGCCCgcgagg                                        >  2:780900/1‑143 (MQ=255)
                                                                                                           cACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAAATAAATTATTTTTTTTTAAATATAGAGATAAACATAACAAAAAAGACAAAATTAAAATTATTAGAGGAGTTAAAGGCAAAAAAAAGGCCAACCGATAggg                                       >  2:3553976/1‑148 (MQ=255)
                                                                                                           cACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATATAATTAAAATATTTTTTTTTTAATATCGAGATAACGATAACAAAAAAGACAAATTTAAAATTATTAGAGGTGTGTAATGGAAAAAAAAGGCCTCCCCGTAggg                                       >  2:1795901/1‑148 (MQ=255)
                                                                                                            aCGTAATTTTTCATATCCTGTCTTTTTTTTTCTATTAAAAAATATAATTAATTTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGa                                      <  1:3553976/148‑1 (MQ=255)
                                                                                                            aCGTAAATTTTCATATCCTGTCTTTTTTTTTCGATTTAAAAATATAATCAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGa                                      <  2:1553229/148‑1 (MQ=255)
                                                                                                              gTACTTTTTCATCTCCTGTCGTTTGATTTCGATTACAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                    <  2:3226592/148‑1 (MQ=255)
                                                                                                                 cATTTTAATATCCTGTCTTTTGTTTTCTATTACAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                                 <  2:647689/148‑1 (MQ=255)
                                                                                                                  aTTTTCATAGCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAATTTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGt                                <  2:1419039/148‑1 (MQ=255)
                                                                                                                     ttCATATCCTGTCGTTTGTTTTGGATTTGAAAATAAAATTAAATTTTTTTTTTTTAAATATCGAGATAAACATAACAAAAAACACAAAATGAAAATTATTAGTGGAGTGCAAGGCAAAAAAAACGCCGCCCCAGAGGGACGCCGTAga                             >  2:2587086/1‑147 (MQ=255)
                                                                                                                           tCCTGTCTTTTGTTTTCTATTAAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                       <  1:2934994/148‑1 (MQ=255)
                                                                                                                               gTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGCCGTAGCCAAGTGcgcg                   >  2:866911/1‑148 (MQ=255)
                                                                                                                                tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                  >  2:3125990/1‑148 (MQ=255)
                                                                                                                                tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACAAAAAAGACAAAATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATATGGAAGCCGTAGCAAAGTGcgcgg                  >  2:3126034/1‑147 (MQ=255)
                                                                                                                                 cGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATAACAAAAAAGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCCATAGGGAAGCCGTAGCAAAGTGCGCgtg                 >  2:738874/1‑148 (MQ=255)
                                                                                                                                   tttgtttTCGATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt               <  2:2782688/148‑1 (MQ=255)
                                                                                                                                    ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt              >  1:402977/1‑148 (MQ=255)
                                                                                                                                       ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa           <  2:279838/148‑1 (MQ=255)
                                                                                                                                         ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg         >  1:3390283/1‑148 (MQ=255)
                                                                                                                                             aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTaagatcg     >  1:394759/1‑142 (MQ=255)
                                                                                                                                             aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTaagatcg     >  1:394761/1‑142 (MQ=255)
                                                                                                                                               ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt   >  1:3391486/1‑148 (MQ=255)
                                                                                                                                               ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt   >  1:3212776/1‑148 (MQ=255)
                                                                                                                                                tCAAAATATAATTAATTTATTTGTTTTTAAATATCTAGATAACGATCAAAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg  <  2:2424091/148‑1 (MQ=255)
                                                                                                                                                tCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg  <  2:812425/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATG  >  CP009273/2170568‑2170859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A