Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,713 C→A 20.0% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7130CA20.0% 357.8 / 28.2 175intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (45/84);  new base A (23/17);  total (74/101)
Fisher's exact test for biased strand distribution p-value = 1.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  CP009273/2170564‑2170862
                                                                                                                                                     |                                                                                                                                                       
gTTGGCAGCGGTTTCTACCACCACGTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTc                                                                                                                                                         <  2:1716522/150‑1 (MQ=255)
 tttgCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                        <  2:3626671/147‑1 (MQ=255)
   ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTTAATGCCGGAACCGCATAACCTCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaa                                                                                                                                                      <  2:263344/150‑1 (MQ=255)
   ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaa                                                                                                                                                      <  2:2529188/150‑1 (MQ=255)
      aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAat‑a                                                                                                                                                  >  2:250161/1‑150 (MQ=255)
       gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATTTTGAATGCCGGAACCGCATAACCTCAGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACAACGTACTTTTTCATATCCTGTCGTTTTTTTTCTATTTCAAAat‑at                                                                                                                                                 <  1:2371116/150‑1 (MQ=255)
        cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAt‑ata                                                                                                                                                <  1:3319999/150‑1 (MQ=255)
         ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATaa                                                                                                                                               <  2:1437622/150‑1 (MQ=255)
         ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAT‑ATaa                                                                                                                                               >  2:2273501/1‑150 (MQ=255)
         ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAAT‑ATaa                                                                                                                                               >  2:3717154/1‑150 (MQ=255)
         ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAaaaaaaaaa                                                                                                                                                >  1:354402/1‑145 (MQ=255)
          gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCCTATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAAt                                                                                                                                              <  2:3952104/150‑1 (MQ=255)
           tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATg                                                                                                                                             <  1:3315080/150‑1 (MQ=255)
             tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGaa                                                                                                                                           <  2:1733193/150‑1 (MQ=255)
             tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGACGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGaa                                                                                                                                           <  2:1301142/150‑1 (MQ=255)
              cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGaaa                                                                                                                                          <  2:2992921/150‑1 (MQ=255)
              cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGaaa                                                                                                                                          <  1:659054/150‑1 (MQ=255)
               tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAat                                                                                                                                         <  2:522704/150‑1 (MQ=255)
                   accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAattatt                                                                                                                                     <  2:658668/150‑1 (MQ=255)
                        ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGttt                                                                                                                                <  2:822304/150‑1 (MQ=255)
                          gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTGATTTAAAAAT‑AAAAATAAAATAAtttttttt                                                                                                                              >  1:3812385/1‑150 (MQ=255)
                          gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTATATTTAAAAAT‑ATAATTAAAtttttttttttt                                                                                                                              >  1:2929647/1‑150 (MQ=255)
                          gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGttttt                                                                                                                              <  2:1897199/150‑1 (MQ=255)
                          gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGACGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGttttt                                                                                                                              <  2:1898706/150‑1 (MQ=255)
                           cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTa                                                                                                                             <  1:2630114/150‑1 (MQ=255)
                           cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTa                                                                                                                             <  1:1924206/150‑1 (MQ=255)
                              cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAat                                                                                                                          <  1:1331306/150‑1 (MQ=255)
                              cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTTATTTAAAAAA‑AAAAATAAAATATTTTTTTTTAAat                                                                                                                          >  1:580944/1‑150 (MQ=255)
                              cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTTTATTTACAAAA‑ATAAAGAAAATATtttttttttttt                                                                                                                          >  1:4321439/1‑146 (MQ=255)
                               gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAata                                                                                                                         <  2:70245/150‑1 (MQ=255)
                               gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAata                                                                                                                         <  1:3621866/150‑1 (MQ=255)
                                 ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATc                                                                                                                       <  2:55250/150‑1 (MQ=255)
                                        ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAT‑ATAATTAAAATAATTTTTTTTAAAAATAGATATaa                                                                                                                >  2:145915/1‑150 (MQ=255)
                                          gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                              <  1:1254511/150‑1 (MQ=255)
                                          gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                              <  2:3340727/150‑1 (MQ=255)
                                          gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                              <  2:2347619/150‑1 (MQ=255)
                                           tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGAATTCAAAAT‑ATAATTAAAATATTTGTTTTTAAAAATAGAGATaaaca                                                                                                             >  2:867191/1‑147 (MQ=255)
                                            gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                            <  1:248067/150‑1 (MQ=255)
                                             aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTGAAAAT‑ATTATTATATTATTTTTTTTTTTATATAGAGTTAActtta                                                                                                           >  1:2256525/1‑146 (MQ=255)
                                             aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTCGCTGTCAAAAA‑ATAAAGAAAATATTTTTTTTTAAAAATAGAGATAACCATc                                                                                                           >  2:3273379/1‑150 (MQ=255)
                                                atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                        <  2:3284570/150‑1 (MQ=255)
                                                    aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAAA‑ATAATGAAAATATTTTTTTTTTAATATAGAGTTAAAGATAACaaaaa                                                                                                    >  2:593552/1‑150 (MQ=255)
                                                        ccGGAACCGCATAACCGCCGCGCTGTGCTTTGTTCAGCATCTGCTTTGACGATACCACGTACATTTTAATATCCTGTCGTTTGTTTTCTATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                                <  2:3861171/150‑1 (MQ=255)
                                                        ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                                <  2:2073883/150‑1 (MQ=255)
                                                         cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACa                                                                                               <  2:633768/150‑1 (MQ=255)
                                                         cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTGGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACa                                                                                               <  2:1430404/150‑1 (MQ=255)
                                                         cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTGAATAT‑ATAAATAAATTATTTTTTTTTTAATATAGATATAAAGaaaaaaaaaaaaaaa                                                                                               >  1:494834/1‑145 (MQ=255)
                                                          ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACaa                                                                                              <  2:1535003/150‑1 (MQ=255)
                                                             aCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGCTTTAAAAATAATAAT‑AAATTTTTTTTTTTTAAAAATCGAGATAAAGATCACAAAAACGACAatat                                                                                           >  2:3107621/1‑150 (MQ=255)
                                                                 cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTTGATTTAAAAAT‑ATAATTAAATTTTTTGTTTTTTAATATCGGGTTAAAGATGAAAAAAAAGACAATATGtaa                                                                                       >  1:2565289/1‑150 (MQ=255)
                                                                  aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAT‑AAAAATAAAATATTTTTTTTTTAATATATAGATAACGATAACAAAAAACACAAAATGtaaa                                                                                      >  2:3422621/1‑150 (MQ=255)
                                                                  aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAACTATTTGTTTTTTAATATCGAGATAACGATCACCAAAACCACAAGATGaaaa                                                                                      >  2:2823021/1‑150 (MQ=255)
                                                                    aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTAAAAAA‑AAAAATTAAATATTTTTTTTTTAAAATAGAGATAAACATAAAAAAAACCACAaaatataaaat                                                                                    >  2:775494/1‑143 (MQ=255)
                                                                              cTGTGCGTTTTTCAGCATCTGCTTTGCCGATACCCCGTACATTTTAATATCCTGTCTTTTTTTTTCGATTTCAAAAT‑ATAATCAAATTTTTTTTTTTTAAATATCGATATACCGATCAAAAAAACGACAATATGAAAATTATTCgaggag                                                                          <  1:1363169/150‑1 (MQ=255)
                                                                              cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgaggag                                                                          <  2:1861334/150‑1 (MQ=255)
                                                                               tgtgCGTTGTTCAGAATCTGCTTTGTCGATCCCACGTAATTTTTCATATCGTGTCGTTTGTTTTCTATTACAAAAT‑ATATTGTAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGt                                                                         <  2:4178842/150‑1 (MQ=255)
                                                                                gtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAT‑ATAATGTAATTATTTTTTTTTTAATATCGAGTTTAAGATCAAAAAAACAAAAAAATAAAAATTATTAGAGGAGtt                                                                        >  1:1806585/1‑149 (MQ=255)
                                                                                 tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGAATTAAAAAA‑AAAATTAAAATAATTTTTTTTTAAAAAAGAGATAAACATAACAAAAAAAACAAAAAGAAAAAAATTAGAGGCGtta                                                                       >  2:3808012/1‑148 (MQ=255)
                                                                                  gCGTTGTACAGCATCTGCTTTGTCGATACCCCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑aa                                                                     <  2:1791827/150‑1 (MQ=255)
                                                                                     ttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAAT‑ATAATGAAATTATTTTTTTTTAAATTTCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAgg                                                                  <  2:4311337/150‑1 (MQ=255)
                                                                                      tgtaCAGCATCTGCTTGGTCGATACCACGTAAATTTTCATATCCTGTCTTTTTTTTGCGATTTCAAAAT‑ATATTTAATTTTTTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGc                                                                 <  2:1916923/150‑1 (MQ=255)
                                                                                       gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAT‑ATAAATAAATTATTTTTTTTTAAATATAGAGATAAAGATAACAAAAAACACAAAATTAAAATTATTAGAGGAGTG‑AAATGCa                                                                >  2:206028/1‑150 (MQ=255)
                                                                                           aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTGAAAAA‑ATAAATAAAATATTTTTTTTTTAATATAGAGATAAACAACACAAAAAAGAAAAAATTTAAAAAATTAGTGGAGTGTGAAGGCaaaa                                                             >  2:2679043/1‑150 (MQ=255)
                                                                                              aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAT‑ATAATGAAATTATTTTTTTTTTAATATAGAGATAAAGATAACAAAAAAGAAAAAATGAAAATTATTAGAGTTGTG‑TATTGCaaaaaaaa                                                         >  2:644798/1‑149 (MQ=255)
                                                                                                cTGTTTTGTCGATCCCAATTAATTTTTAATATCTTTTCTTTTTTTTTCTATTAAAAAAT‑ATAATTAATTTTTTTTTTTTTAAATATCTATAAAACGAAAAAAAACACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACgg                                                       <  1:867863/150‑1 (MQ=255)
                                                                                                cTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACgg                                                       <  1:1840524/150‑1 (MQ=255)
                                                                                                cTGATTTTTCGATACCACTTAAATTTTCATATCCTGTCGTTTGTTTTCTATTACAAAAT‑ATAATGAATTTATTTTTTTTTAATTATCGATATAACGACCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACgg                                                       <  2:1559435/150‑1 (MQ=255)
                                                                                                 tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTATATATCGAGATAAAGATAACAAAAACGACAATATGAAAATTATTCGCGGAGTG‑AAAGGCCAAAATACGGc                                                      >  2:4148979/1‑150 (MQ=255)
                                                                                                 tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAT‑ATAATGAAAATAATTTTTTTTTAAAATAGAGATAACGATAACAAAAACAAAAAAATGAAAATTTATAGAGGAGTG‑TAATGCAAAAAAAAGGc                                                      >  2:4010831/1‑150 (MQ=255)
                                                                                                      tGTCGATCCCACTTACTTTTAAATAACTTGTCGTTTTTTTTCTTTTTAAAAAT‑TAATTTAATTTTTTTTTTTTTAATTATCTATATAACTAAAACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTccc                                                 <  2:3785456/150‑1 (MQ=255)
                                                                                                         ctaTACCACTTACTTTTTCATATCCTTTCTTTTTTTTTCTATTTAAAAAT‑ATAATTAAATTTTTTTTTTTTAAATTTCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGAt                                              <  2:1353530/148‑1 (MQ=255)
                                                                                                         cGATACCACGTACATTTTCATATCCTTTCGTTTGTTTTCGATTTCAAAAT‑ATAATTAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGAt                                              <  2:1352224/150‑1 (MQ=255)
                                                                                                          gATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAT‑ATAATGAAATTATTTTTTTTTAAAAATAGAGATAACGATCACAAAAACGCCAATATTAAAATTATTGGGGGAGTG‑AAAGGCAAAAAAAAGGCCTCCCGaga                                             >  2:4022462/1‑148 (MQ=255)
                                                                                                           aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAg                                            <  2:2163261/150‑1 (MQ=255)
                                                                                                           aTACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTCAAAAT‑ATAAATAAAATATTTTTTTTTTTAAATAGAGATAAAGAGAACAAAAAACACCAAATGTAAATTAATAGAGGGGTG‑AAAGGCAAAAAAAAAGCCAcccccgag                                            >  2:455974/1‑145 (MQ=255)
                                                                                                            aaCCACTTAAATTTTAATATCCTGTCTTTTTTTTTCTATTTAAAAAT‑ATATTGAAATTATTTTTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAgg                                           <  1:3023716/149‑1 (MQ=255)
                                                                                                             acaaCGTAAATTTTCATATCCTGTCGTTTTTTTTGTATTTCAAAAT‑ATAATTAATTTATTTTTTTTTAAATATCTTTATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAggg                                          <  1:2961047/147‑1 (MQ=255)
                                                                                                                     ctttttCAGAGCCTGTCGTTTGTTTTCGATTACAAAAT‑ATAATGAATTTTTTTGTTTTTAAATATCGAGATAACTCTCACAAACACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  2:3082406/148‑1 (MQ=255)
                                                                                                                     cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  2:4488734/150‑1 (MQ=255)
                                                                                                                     atttttCATATCTTGTGTTTTTTTTTCTATTAAAAAAT‑ATAATTAAATTATTTTTTTTTAAATATCTATATAACGATCACAACCACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  1:973928/148‑1 (MQ=255)
                                                                                                                     atttttCATACCATGTGTTTTTTTTTCTATTAAAAAAT‑ATAATTAATTTTTTTTTTTTTAAATTTCGATAAAAAGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  1:3238521/148‑1 (MQ=255)
                                                                                                                          tCATATCCTGTCGTGTGTTTTGGATTTAAAAAA‑AAAAATAAAATATTTTTTTTTTAATAAAGACAAAAAGAACACAAAAAAGACAAAAATAAAATTATTAGAGGAGTG‑AAAGGCAAAAAAAAAGCCACCCCAAAAGGAAGCCCGAGCaaa                             >  2:152040/1‑150 (MQ=255)
                                                                                                                             tatCCTTTTTTTTTTTTTCTTTTAAAAAAT‑ATAAATAAATTTTTTTTTTTTAAATATCTATAAAAGTAAAAAAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                          <  1:858411/150‑1 (MQ=255)
                                                                                                                              tcccTGTCGTTTTTTTTCGATTAAAAAAT‑AAAATGAAATTATTTTTTTTTAAATATCTATATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTga                         <  1:4412155/148‑2 (MQ=255)
                                                                                                                                cgtGTCTTTTGTTTTCTATTTCAAAAT‑ATAATGAATTTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  2:3882431/148‑1 (MQ=255)
                                                                                                                                   gTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGCGGAGTG‑AAAGGCAAAAAAACGGCCGCCCGATAGGGAAGCCGTCGCAAAGTGCGCgtg                    >  1:3904991/1‑150 (MQ=255)
                                                                                                                                   gTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                    >  2:3221570/1‑150 (MQ=255)
                                                                                                                                         tgtttTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa              >  1:195279/1‑150 (MQ=255)
                                                                                                                                          gtttTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             >  1:2409653/1‑150 (MQ=255)
                                                                                                                                          gtttTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             >  1:676077/1‑150 (MQ=255)
                                                                                                                                          gtttTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             >  1:676087/1‑150 (MQ=255)
                                                                                                                                           ttttCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            >  1:2217388/1‑150 (MQ=255)
                                                                                                                                            tttCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           >  1:766888/1‑150 (MQ=255)
                                                                                                                                                taTTTAAAAAT‑ATAATGAAATTTTTTTTTTTTAAATATCTAGATAACGAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa       <  1:1994752/149‑1 (MQ=255)
                                                                                                                                                gATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa       >  1:764121/1‑150 (MQ=255)
                                                                                                                                                 aTTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt      >  1:3449752/1‑150 (MQ=255)
                                                                                                                                                 aTTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt      >  1:4445950/1‑150 (MQ=255)
                                                                                                                                                     cAAAAT‑ATAATTAAATTATTTTTTTTTAATTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  <  2:3915226/150‑1 (MQ=255)
                                                                                                                                                     cAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  1:3662286/1‑150 (MQ=255)
                                                                                                                                                     cAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  1:3769135/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAT‑ATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTG‑AAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  CP009273/2170564‑2170862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A