Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F17 I0 R1 93 279.2 9183790 95.6% 8779703 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,713 C→A 28.6% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7130CA28.6% 349.7 / 34.1 180intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (42/75);  new base A (28/27);  total (78/102)
Fisher's exact test for biased strand distribution p-value = 6.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

GCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCG  >  CP009273/2170568‑2170861
                                                                                                                                                  |                                                                                                                                                     
gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTAGATTTaaaa                                                                                                                                                    >  1:1996983/1‑149 (MQ=255)
 cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCaaaa                                                                                                                                                   <  1:908368/149‑1 (MQ=255)
  aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAat                                                                                                                                                  <  1:3021137/149‑1 (MQ=255)
  aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAat                                                                                                                                                  <  2:3063038/149‑1 (MQ=255)
  aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAat                                                                                                                                                  <  2:3964859/149‑1 (MQ=255)
   gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAata                                                                                                                                                 <  1:295871/149‑1 (MQ=255)
         tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAAaataatta                                                                                                                                           >  2:2274131/1‑147 (MQ=255)
           tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                         <  1:1929354/149‑1 (MQ=255)
            accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                        <  1:4238067/149‑1 (MQ=255)
                ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    <  2:3010550/149‑1 (MQ=255)
                ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    <  1:2636768/149‑1 (MQ=255)
                 cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCTCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                   <  2:1768959/149‑1 (MQ=255)
                 cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                   <  1:716832/149‑1 (MQ=255)
                  acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTg                                                                                                                                  <  1:2277863/149‑1 (MQ=255)
                  acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTTGATTTAAAAAAATAATTAAATAAtttt                                                                                                                                  >  1:3534162/1‑148 (MQ=255)
                   cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                                 <  2:2060401/149‑1 (MQ=255)
                   cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                                 <  1:3072796/149‑1 (MQ=255)
                    ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                <  1:1378224/149‑1 (MQ=255)
                     tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                               <  2:3589886/149‑1 (MQ=255)
                       cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGttttt                                                                                                                             <  2:2704025/149‑1 (MQ=255)
                       cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGttttt                                                                                                                             <  1:1340009/149‑1 (MQ=255)
                         tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAGAATTCAAAAAATAATTAAATTATTTTTTTTTaa                                                                                                                           >  2:4102089/1‑149 (MQ=255)
                         tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                           <  1:4139007/149‑1 (MQ=255)
                         tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGAATTAAAAAAAAAAAGAAATTAATTTTTTTTaa                                                                                                                           >  2:1804501/1‑149 (MQ=255)
                           gtgtCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGAGACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAat                                                                                                                         <  1:4230131/146‑1 (MQ=255)
                           gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAGATTTAAAAAAATAATGAAATTAATTTTTTTTAAat                                                                                                                         >  2:2448972/1‑149 (MQ=255)
                            tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAata                                                                                                                        >  2:1477071/1‑149 (MQ=255)
                            tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGATTTAAAAAAATAAAGAAAATATttttttttttata                                                                                                                        >  1:3278633/1‑144 (MQ=255)
                             ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGATTTAAAAAAATAATGTAATTATTTTTTTTTTTatat                                                                                                                       >  2:3878951/1‑149 (MQ=255)
                              tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                      <  1:2529312/149‑1 (MQ=255)
                              tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                      <  1:4407779/149‑1 (MQ=255)
                              tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTTAatata                                                                                                                      >  2:3461574/1‑148 (MQ=255)
                               cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg                                                                                                                     <  2:2528191/149‑1 (MQ=255)
                                gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                    <  2:2275250/149‑1 (MQ=255)
                                gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                    <  2:788093/149‑1 (MQ=255)
                                gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGATTTAAAAAAAAAATTAAAATATTTTTTTTTAAatatata                                                                                                                    >  2:2355736/1‑146 (MQ=255)
                                 agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTCGATTTGAAAATATAATTAAATTTTTTTTTTTTAAATATagag                                                                                                                   >  2:913823/1‑149 (MQ=255)
                                 agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTAGATTTAAAAAAAAAAATAAAATATTTTTTTTTTAataaaaat                                                                                                                   >  1:2199164/1‑144 (MQ=255)
                                  gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAGATTTAAAAAAATAATTAAATTATTTTTTTTTTAatttagata                                                                                                                  >  2:3899167/1‑142 (MQ=255)
                                  gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag‑a                                                                                                                 <  2:1617572/149‑1 (MQ=255)
                                   aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑At                                                                                                                <  2:507344/149‑1 (MQ=255)
                                    ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTAAAAAAAAAATGAAAATATTTTTTTTTAAATATAAAG‑ATa                                                                                                               >  1:4549159/1‑149 (MQ=255)
                                      gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAAc                                                                                                             <  2:3721659/149‑1 (MQ=255)
                                           tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAGATTTAAAAATAAAAATAAAATATTTGTTTTTAAATATAGAG‑ATaaacataa                                                                                                        >  2:163994/1‑143 (MQ=255)
                                           tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATca                                                                                                        <  1:609988/149‑1 (MQ=255)
                                            atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAG‑ATAAAGATaac                                                                                                       >  2:3399544/1‑149 (MQ=255)
                                            atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATcac                                                                                                       <  1:897001/149‑1 (MQ=255)
                                            atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTAAAAATATAATTAAAATATTTTTTTTTATATATAGAG‑ATaaacataac                                                                                                       >  2:4360565/1‑142 (MQ=255)
                                                 aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAAAGAAATTATTTTTTTTTAAATATAGAG‑ATAACGATCACaaaaa                                                                                                  >  2:4570766/1‑149 (MQ=255)
                                                    ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGa                                                                                               <  2:1343753/149‑1 (MQ=255)
                                                      ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTCAAAATATAATTTAATTATTTTTTTTTAAATATAGAG‑ATAAAGATCAAAAAAAAGaaa                                                                                             >  2:1083198/1‑147 (MQ=255)
                                                       gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTGTTTT‑GTTTTAAAAATATAAATAAAATATTTTTTTTTTTATATATAT‑ATAAAGATAACaaaaaacaaaa                                                                                            >  1:3910486/1‑143 (MQ=255)
                                                         aCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAGATTTCAAAAAAAAATTTAAATATTTTTTTTTTAATATAGAT‑ATAACGATCACAAAAAAAACaaaa                                                                                          >  1:1039444/1‑147 (MQ=255)
                                                         aCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGTTTTAAAAATAAAATTAAAATATTTTTTTTTTAATATAGAGAATAACGAAAACAAAAAcaacaaa                                                                                           >  1:1039302/1‑148 (MQ=255)
                                                           cgaaTAACCGCCGCGCTGTGCGTTGTTCAGCATCTTCTTTTTCGATACCACGTACATTTTCATATCCTGTCTT‑TTGTTTTCTATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCTAG‑ATAACGATCACAAAAACGACAATATg                                                                                        <  2:3751493/146‑1 (MQ=255)
                                                              aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTTGATTTAAAAATATAATTAAAATTTTTTTTTTTTTATATATAG‑TTAAAGATAAAAAAAAAGACAAAATTaaa                                                                                     >  2:5090/1‑149 (MQ=255)
                                                               tAACCGCCGCGCTGTGCTTTGTTCAGCATCTGCTTTGTCTATACCACTTAAATTTTCTTATCCTGTCTT‑TTTTTTTCTATTTAAAAATATAATGAATTTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGaaaa                                                                                    <  2:593261/149‑1 (MQ=255)
                                                               tAACCGCCGCGCTGTGCGTTGTTCAGCATCTTCTTTGTCGATACCACGTACATTTTAATATCCTGTCGT‑TTGTTTTCTATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAACACGACAATATGaaaa                                                                                    <  2:2882459/149‑1 (MQ=255)
                                                                 aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGGTTTAGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAatt                                                                                  >  2:3014365/1‑149 (MQ=255)
                                                                 aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAG‑ATAACGATCACAAAAACGACAATATGaaaaag                                                                                  >  2:3014556/1‑147 (MQ=255)
                                                                         gcTGTGCGTTGTTCAGCATCTGCTTTGTCTATACCACGTAATTTTTCATATCCTGTCTT‑TTGTTTTCGATTAAAAAATATAATCAATTTTTTTGTTTTTAATTATCGAT‑ATAACGATAAAAAAAACGACAATATGAAAATTATTCgagg                                                                          <  1:2598972/149‑1 (MQ=255)
                                                                          cTGTGTGTTGTTCAGCATCTGCTTTGTCGATACCACGAACATTTTCATATCCTGTCTT‑TTGTTTTCGTTTTAAAAATATAATTAAATTTTTTTTTTTTAAATATCTAG‑ATAACGATCAAAAACACGACAATATGAAAATTATTCgagga                                                                         <  1:2514839/149‑1 (MQ=255)
                                                                           ggggCGTTGTTCAGCATCGGCTTTGTCGAACCCACGTACTTTTTCATAGCCGTGCGT‑TTGTTTTCTTTTAAAAAATATAATTAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCgaggag                                                                        <  2:2679373/146‑1 (MQ=255)
                                                                            gtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTCTATTTGAAAATATAAATAAATTATTTTTTTTTAAATATAGAG‑ATAACGATCAAAAAAAAAAAAAAATTAAAATAATTCGAGGAGt                                                                       >  1:2648156/1‑149 (MQ=255)
                                                                               cGTTGTTCATCATCTGTTTTGTCGACACCACGTAATTTTAAATATCCTGTTTT‑TTTTTTTCTATTAAAAAATATAATTAAATTTTTTGTTTTTAAATATCGAG‑ATACCTAACAAAAACACGACAATATGAAAATTATTCGAGGAGTGaa                                                                    <  1:1610032/149‑1 (MQ=255)
                                                                                gttgttCAGCATCTGATTTGTCGATACCACGTAAATTTTCATATCCTGTCGT‑TTGTTTTCTATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                   <  2:2063149/149‑1 (MQ=255)
                                                                                      ctgCATCTGCTTTGTCGATCCCACGTACATTTTCATAGCCTGTCGT‑TTGTGTTCGATTTCAAAATTTAATGAAATTATTTTTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                             <  2:4482274/147‑1 (MQ=255)
                                                                                        gCATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGT‑TTGTTTTCTATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAG‑ATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaa                                                           <  2:2393538/149‑1 (MQ=255)
                                                                                        gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCGATTTCAAAAAAAAAAGCAATTATTTGTTTTTAAAAATAGAG‑ATAACCATCACAAAAACCACAAAATGAAAATTATTCGAGGCGTGAAAGGCaaaaa                                                           >  2:4034783/1‑149 (MQ=255)
                                                                                        gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCGATTTCAAAAAAAAAAGAAATTAATTTTTTTTAAAAATAGAG‑AAAACGATCACAAAAAACACAAAAAGTAAAATAATCGAGGAGTGAAACGCcaaaa                                                           >  2:4034565/1‑149 (MQ=255)
                                                                                        ccATTTGCTTTTTCTATACCACTTAATTTTTAATATCCTTTTTT‑TTTTTTTCTATTAAAAAATATAATCAATTTTTTTTTTTTAAAATATCTAT‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaa                                                           <  1:4429349/148‑1 (MQ=255)
                                                                                          aTCTGCTTTGTCTATACCCCGTAATTTTTCATATCCTGTCGT‑TTTTTTTCTATTACAAAATATAATTAAATTATTTTTTTTTAAATATCGAG‑ATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                         <  1:3783637/149‑1 (MQ=255)
                                                                                           tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGTATTTAAAATATAATTAAAAAATTTTTTTTTAAATATATTT‑ATAAAGAAAAAAAAAAATAAAAAATGAAAATTATTATAGGAGTTATATTCaaaaaaaa                                                        >  1:1599864/1‑148 (MQ=255)
                                                                                               cTTTGTCTATACCACGTAAATTTTCATATCCTGTCTT‑TTTTTTTCTATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGAT‑ATAACTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGcc                                                    <  2:347075/149‑1 (MQ=255)
                                                                                                  tGTCGATCCCACTTACTTTTTAATTTCCGTTGTT‑TTTTTTTCTTTTAAAAAATTTATTTAATTTTTTTTTTTTTAAATTTCTAT‑ATACCTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                 <  1:3023280/149‑1 (MQ=255)
                                                                                                   gTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGATTTAAAAATATAATGAAAATATTTGTTTTTAAATATAGAG‑ATAACGATCACAAAAACGAAAATATGAAAATTATTAGAGGGGTGAAAGGCAAAAAAAACGCCTccc                                                >  2:2992826/1‑149 (MQ=255)
                                                                                                     ctacaCCACTTAATTTTTAATATCCTGTGTT‑TTGTTTTCTTTTAAAAAATATAATTAATTTATTTGTTTTAAAATATCGAT‑ATAACGATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                              <  1:4484905/145‑1 (MQ=255)
                                                                                                     ctaaaacACTTAAATTTTATTATCTTTTAGT‑TTTTTTTCTATAAAAAAATAAAATTAATTTTTTTTTTTTAAAATATCTAT‑AAAAATATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                              <  1:4257293/143‑1 (MQ=255)
                                                                                                              gTACATTTTCATATCCTGTCGT‑GTGTTTTAGATTTCAAAATATAAAGTAATTATTTTTTTTTTAATATGGAG‑CTTACGGTCGCAAAAAAGGCCATAGTGAAAGTATTCGGGGAGGGGAAGGCACAAGACCGGCCTCCCGATCGGGGAGc                                     >  2:3328949/1‑149 (MQ=255)
                                                                                                                        atatCCTGTCTT‑TTTTTTTCTATTAAAAAATAAAATTAAATTATTTTTTTTTAAATATCTAT‑ATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAg                           <  1:1815438/149‑1 (MQ=255)
                                                                                                                                  gt‑ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                 >  1:2754309/1‑149 (MQ=255)
                                                                                                                                  gt‑ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAGAGGGAAGCCGTAGCAAAGTGCGCGTGtt                 >  2:4108586/1‑149 (MQ=255)
                                                                                                                                   t‑ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                >  1:1726926/1‑149 (MQ=255)
                                                                                                                                    gttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCAAAGTGCGCGTGttt                >  1:4032616/2‑149 (MQ=255)
                                                                                                                                     tttttttCGTTTAAAAAATTTAATCAATTTATTTTTTTTTAAATTACGAG‑AAACCGAAAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt               <  1:2355736/146‑1 (MQ=255)
                                                                                                                                      ttttttCTATTAAAAAATATAATTAATTTATTTTTTTTAAAATATCTAT‑ATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt              <  1:2124832/147‑1 (MQ=255)
                                                                                                                                       tttttCTATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa             <  2:4037375/148‑1 (MQ=255)
                                                                                                                                       gtttTCGATTAAAAAATATAATGTAATTATTTTTTTTTAATTATAGTG‑ATAAGTATCACAAAAACGACAATATGATAATTATTCGAGGAGTGAATGGCAAAAAAACGTCCTCCCTATAGGGAAGCCGTTGCAAATTGCGCGTGTTTTTa             <  2:1881038/149‑1 (MQ=255)
                                                                                                                                       gtttTCCATTACAAAATATAATTAAATTTTTTTTTTTTAAATATCGAT‑ATAACGAACAAAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa             <  1:4339455/149‑1 (MQ=255)
                                                                                                                                         tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg           >  2:364810/1‑149 (MQ=255)
                                                                                                                                          ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc          >  1:4371498/1‑149 (MQ=255)
                                                                                                                                             gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg       >  1:2587162/1‑149 (MQ=255)
                                                                                                                                              ttttAAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa      <  2:1996988/148‑1 (MQ=255)
                                                                                                                                              aTTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa      <  2:675306/149‑1 (MQ=255)
                                                                                                                                              aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa      >  1:1402993/1‑149 (MQ=255)
                                                                                                                                              aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa      >  1:1402811/1‑149 (MQ=255)
                                                                                                                                              aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa      >  1:3543825/1‑149 (MQ=255)
                                                                                                                                              aTTACAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa      <  2:4300893/149‑1 (MQ=255)
                                                                                                                                               tttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt     >  1:1130957/1‑149 (MQ=255)
                                                                                                                                                  cAAAATATAATAAAATTATTTTTTTTTAAATATCGAT‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg  <  1:3739224/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAG‑ATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCG  >  CP009273/2170568‑2170861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A