Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F14 I0 R1	127	310.1	10402230	94.2%	9798900	149.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,170,713	C→A	21.1%	intergenic (‑30/+278)	gatY ← / ← fbaB	D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,170,713	0	C	A	21.1%	550.6 / 30.0	257	intergenic (‑30/+278)	gatY/fbaB	D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand): ref base C (53/128); new base A (48/13); total (116/141)
Fisher's exact test for biased strand distribution p-value = 1.70e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCG  >  CP009273/2170565‑2170861

ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTc                                                                                                                                                         <  1:904596/149‑1 (MQ=255)

ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTc                                                                                                                                                         <  2:3452914/149‑1 (MQ=255)

ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTc                                                                                                                                                         <  1:1846058/149‑1 (MQ=255)

   gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCaaa                                                                                                                                                      <  2:736786/149‑1 (MQ=255)

    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCaaaa                                                                                                                                                     <  1:2673418/149‑1 (MQ=255)

     aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCG‑ATTTAAAAat                                                                                                                                                    >  2:3828025/1‑149 (MQ=255)

      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAat‑a                                                                                                                                                  <  1:3046852/149‑1 (MQ=255)

      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAG‑ATTTAAAAat‑a                                                                                                                                                  >  2:131833/1‑149 (MQ=255)

       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAat‑at                                                                                                                                                 <  1:340202/149‑1 (MQ=255)

         gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATaa                                                                                                                                               <  2:1687570/149‑1 (MQ=255)

         gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCG‑CTTTCAAAAA‑ATaa                                                                                                                                               >  2:3211656/1‑149 (MQ=255)

         gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GGGTTTTGG‑GTTTTAAAAA‑ATaa                                                                                                                                               >  1:3653608/1‑149 (MQ=255)

         gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GGGTGTTTT‑TTTTGAAAAT‑ATaa                                                                                                                                               >  1:1280837/1‑149 (MQ=255)

          tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAAt                                                                                                                                              <  1:4438969/149‑1 (MQ=255)

          tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAAt                                                                                                                                              <  2:3731901/149‑1 (MQ=255)

           ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATg                                                                                                                                             <  2:888112/149‑1 (MQ=255)

           ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATg                                                                                                                                             <  2:1044139/149‑1 (MQ=255)

            tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGa                                                                                                                                            <  2:2722688/149‑1 (MQ=255)

            tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGa                                                                                                                                            <  2:3742332/149‑1 (MQ=255)

             cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGaa                                                                                                                                           <  2:3323187/149‑1 (MQ=255)

              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGaaa                                                                                                                                          <  2:3595155/149‑1 (MQ=255)

              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGaaa                                                                                                                                          <  1:4354402/149‑1 (MQ=255)

               accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAat                                                                                                                                         <  1:2682991/149‑1 (MQ=255)

               accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAat                                                                                                                                         <  1:996134/149‑1 (MQ=255)

               accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCG‑AATTGAAAAT‑ATAAAGAAat                                                                                                                                         >  2:4388402/1‑149 (MQ=255)

                ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTTG‑ATTTAAAAAA‑ATAATTAAat‑t                                                                                                                                       >  2:1752002/1‑149 (MQ=255)

                    cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAG‑AGTTATAAAT‑AAAATTAAAT‑TAttt                                                                                                                                   >  1:1133376/1‑149 (MQ=255)

                     acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGTAAT‑TATTTg                                                                                                                                  <  2:3058861/149‑1 (MQ=255)

                      cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAT‑ATATCAAAAA‑AAAAAGAAAA‑TAATTGt                                                                                                                                 >  1:1106821/1‑149 (MQ=255)

                       ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGtt                                                                                                                                <  1:4143721/149‑1 (MQ=255)

                         gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGtttt                                                                                                                              <  2:584740/149‑1 (MQ=255)

                          cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGttttt                                                                                                                             <  1:803564/149‑1 (MQ=255)

                           aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTGTTTT‑G‑ATTTAAAAAA‑ATAATTAAAA‑TATATTTTTTTa                                                                                                                            >  1:4966693/1‑149 (MQ=255)

                           aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTa                                                                                                                            <  1:3890370/149‑1 (MQ=255)

                           aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAG‑ATTTAAAAAA‑ATAATTAAAA‑TATttttttttt                                                                                                                            >  1:769370/1‑148 (MQ=255)

                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAG‑ATTTAAAAAT‑ATAAATAAAT‑TAATTTTTTTTaa                                                                                                                           >  2:4624026/1‑149 (MQ=255)

                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAG‑ATTTAAAAAT‑ATAAATAAAT‑TAATTTTTTTTaa                                                                                                                           >  2:2947166/1‑149 (MQ=255)

                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAata                                                                                                                        <  2:1084320/149‑1 (MQ=255)

                                    agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAG‑ATTTAAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCgag                                                                                                                   >  2:431607/1‑149 (MQ=255)

                                    agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAG‑AATTCAAAAT‑ATAAATAAAA‑TATTTTTTTTTTAatatagtg                                                                                                                   >  2:1451094/1‑145 (MQ=255)

                                    agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGG‑ATTTAAAAAA‑ATAATTAAAT‑TATTTTTTTTTTAATATagag                                                                                                                   >  2:4788686/1‑149 (MQ=255)

                                     gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCgaga                                                                                                                  <  1:2640763/149‑1 (MQ=255)

                                     gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTTGTTTTTAAAAAA‑AAAAATAAATATATTTTTTTTTatatataaa                                                                                                                    >  1:3062224/1‑146 (MQ=255)

                                     gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCT‑ATTTCAAAAT‑ATAATGAAAT‑TATTTTTTTTTAAATATAgaga                                                                                                                  >  2:718625/1‑149 (MQ=255)

                                       ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATa                                                                                                                <  2:3569042/149‑1 (MQ=255)

                                        tgtgAATATCGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATaa                                                                                                               <  2:4742796/149‑1 (MQ=255)

                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAAc                                                                                                              <  2:169658/149‑1 (MQ=255)

                                          tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATAGAGGTAACg                                                                                                             >  2:3799213/1‑149 (MQ=255)

                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGa                                                                                                            <  2:2074377/149‑1 (MQ=255)

                                            aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAt                                                                                                           <  2:4076970/149‑1 (MQ=255)

                                             atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑c                                                                                                         <  1:4515834/149‑1 (MQ=255)

                                             atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑c                                                                                                         <  2:3623930/149‑1 (MQ=255)

                                               atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑cac                                                                                                       <  1:4704468/149‑1 (MQ=255)

                                                ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAG‑ATTTAAAAAA‑ATAAATAAAA‑TATTTTTTTTTATATATCGAGATAAAGAT‑aaca                                                                                                      >  1:5186311/1‑149 (MQ=255)

                                                   aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTTG‑ATTTTAAAAT‑ATAATTAAAA‑TATTTTTTTTTTAATATAGAGATAACGAT‑AACaaaa                                                                                                   >  2:3927907/1‑149 (MQ=255)

                                                        cGGAACCGCATAACCGCCGCGCTGTGCGTTTTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCT‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGAc                                                                                              <  2:2974745/149‑1 (MQ=255)

                                                         ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGG‑ATTTAAAAAT‑ATAAATAAAA‑TAATTTTTTTTTAATATAGAGGTAAAGAA‑AACaaaaaaaaaa                                                                                             >  1:2056954/1‑144 (MQ=255)

                                                          gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTTTTTTTATATATAGAGATAAAGAT‑CACAAAAACGACaa                                                                                            >  2:4877608/1‑149 (MQ=255)

                                                          gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAG‑ATTTAAAAAA‑ATAAATAAAT‑TATTTTTTTTTTTATATTGAGATAAAGAT‑CACAAAAACCACaa                                                                                            >  2:4003476/1‑149 (MQ=255)

                                                               gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTTG‑ATTTGAAAAT‑AAAAAGAAAA‑TATTTTTTTTTTAATATAGAGATAAAGAT‑AAACAAAACGACAAAATGa                                                                                       >  1:1202034/1‑149 (MQ=255)

                                                                  tAACCGCCGGGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCCCGTACATTTTAATATCCTGTCGT‑TTGTTTTCT‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGaaaa                                                                                    <  2:3368023/149‑1 (MQ=255)

                                                                     ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTAAAAAA‑ATAAAGAAAT‑TATTTTTTTTTAAATAACGAGATAACCAA‑AACAAAAACGACAATATGAAAatta                                                                                 >  2:2532941/1‑149 (MQ=255)

                                                                     ccgccgCGCTGTGCGTTGTTCAGCATCTGATTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAatta                                                                                 <  2:407416/149‑1 (MQ=255)

                                                                          gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTTT‑TTTTTAAAAA‑AAAAATAAAT‑TATTTTTTTTTTAAAATATATATAAAAAA‑AACAAAAAACACAAAAATAAAAATATTGga                                                                            >  1:4889219/1‑149 (MQ=255)

                                                                            gcTGTGCGTTGTTCCGCATCTGCTTTGTCGATACCCCGAACATTTTCATATCCTTTCGT‑TTGTTTTCG‑ATTAAAAAAT‑ATAATTAAAT‑TATTTTTTTTTAAATATCGAGATACCGAA‑CACAAACACGACAATATGAAAATTATTCgagg                                                                          <  1:2508777/149‑1 (MQ=255)

                                                                            gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTTTTTTTAAATATCGAGATAACGAT‑CACAAAAAAGCCAATATGTAAATTATTAgagg                                                                          >  2:5136293/1‑149 (MQ=255)

                                                                                   gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAG‑AATTAAAAAA‑ATAAAGAAAT‑TATTTTTTTTTAAATATAGAGATAACGAT‑AAAAAAAACGAAAAAATTAAAATTATTAGAGGAGTGaaa                                                                   >  2:2857427/1‑149 (MQ=255)

                                                                                   gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTTT‑TTTTAAAAATAAAAATAAAAT‑AATTTTTTTTTAAA‑AAATAGATAAAAAA‑AAAAAAAAAGACAATAATAAAATTATTAGAAGAGttgta                                                                   >  1:1583049/1‑145 (MQ=255)

                                                                                             aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTGT‑TTTTAAAAAA‑ATAATTAAAA‑TTTTTTTTTTTTAATATAGGGATTAACAA‑CACAAAAACCACAAAAATTAAATTAATAGAGGAGTGTAAGGGcaaaaaa                                                         >  2:3492374/1‑149 (MQ=255)

                                                                                             aTCTGCTTTGTCGATACCACGTACATTTTAATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATTAAAT‑TATTTGTTTTTAAATATCGATATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                         <  2:2792899/149‑1 (MQ=255)

                                                                                               cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTCG‑ATTTAAAAAT‑ATAATTAAAA‑TATTTGTTTTTAAATATAGAGATAACGAA‑CACAAAAAAGACAAAATGAAAATTATTAGAGGGGTGAAATGCAAAAAAAca                                                       >  2:2024180/1‑148 (MQ=255)

                                                                                                     tGTCTATACCACTTAAATTTTCATATCCTGTCGT‑TTTTTTTCT‑TTTTCAAATT‑ATAATTAAAT‑TTTTTTTTTTTAATTTTCTTTAAAACGAT‑AACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                 <  2:2181645/149‑1 (MQ=255)

                                                                                                           tACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGG‑ATTTAAAAAA‑AAAAATAAAA‑TATTTTTTTTTAAATAAAGAGATAAACAA‑AACAAAAACCACACAAAGAAAAAAAATAGAGGCGGGGAAGGCAAAAAAACCGCCAcccccaag                                           >  1:3370571/1‑144 (MQ=255)

                                                                                                             ccACGTAAATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                         <  2:4577850/149‑1 (MQ=255)

                                                                                                               aCGTACATTTTCATATCCTGTCGT‑TTTTTTTAG‑ATTTAAAAAT‑ATAATTTAAT‑TTTTTTTTTTTAAATTTCGTGATAAAGAT‑AACAAAAAAGACAATTTGTAAATTTTTAGAGGTGGGTAAGGGCAAATAACGGCCTCCCGAGAgggga                                       >  2:3410519/1‑147 (MQ=255)

                                                                                                                cGTACATTTTCATATCCTGTCGT‑TTGTTTTAG‑ATTTGAAAAT‑ATAATGAAAA‑TATTTTTTTTTAAATATCTCGATAACCAT‑AACAAAAAAGTCAATATTAAATATATTCTGGGAGTGAAATGCAAAAAAAAGGCCACCCGATAGGGGAg                                      >  2:2284561/1‑149 (MQ=255)

                                                                                                                cGTACATTTTCATATCCTGTCGT‑GTGTTTTCG‑ATTTAAAAAT‑ATAATTTAAT‑TATTTTTTTTTAAAAATCGAGATAACGAT‑AACAAAAACGACAATATGAAAATTATTCGAGGAGTGGAAGGCAAAAAAACGGCCACCCGATAGGGAAg                                      >  2:812216/1‑149 (MQ=255)

                                                                                                                   aaaTTTTCATAGCCTGTCGT‑TTGTTTTCG‑ATTTAAAAAT‑ATAATGAAAT‑TATTTTTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                                   <  2:2889216/147‑1 (MQ=255)

                                                                                                                   aCATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATATTGAACT‑TATTTTTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                                   <  2:3653608/149‑1 (MQ=255)

                                                                                                                     aTTTTCATATCCTGTCGT‑TTGTTTTAG‑ATTTAAAAAA‑ATAAATAAAA‑TATTTGTTTTTAAATATAGAGATAACGAT‑CAAAAAAAAGAAAAAAATTAAAATATTAGAGGAGTGAAAGGCAAAAAAACCGCCAACCCATAGGGAAAcccga                                 >  2:416119/1‑146 (MQ=255)

                                                                                                                     aTTTTCATATCCTGTCGT‑GTGTTTTAG‑ATTTAAAAAA‑ATAATTAAAT‑TATTTTTTTTTAAATATCGAGATAACGAT‑CACAAAAAACACAAAATTAAAATTATTCGAGGAGTGAAAGGCCAAAAAAAGGCCACCCGATAGGGAAGCCgaa                                 >  2:2903829/1‑147 (MQ=255)

                                                                                                                           ttatCGTGTTTT‑TTTTTTTCT‑TTTAAAATAT‑ATATTTATAT‑TTTTTTTTTTTAAATATCTATATATCTAA‑AACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAg                           <  1:152777/148‑1 (MQ=255)

                                                                                                                             atCCTGTCGT‑TTGTTTTAG‑ATTTAAAAAT‑ATAATGAAAT‑TATTTTTTTTTAAAAATATAGATAACGATACAAAAAAAC‑ACAAAAAGAAAATTATTAGAGGAGTGAAATGCAAAAAAAAGGCCACCCCATAGGGAAACCCTAGCCAAGTg                         >  2:4519433/1‑149 (MQ=255)

                                                                                                                              tCCTTTCTT‑TTTTTTTCT‑ATTAAAAAAT‑ATAATTATTT‑TTTTTTTTTTAAATTATCTATAAAACTAA‑CAAAACAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                        <  1:1348954/149‑1 (MQ=255)

                                                                                                                                 tgtgtt‑tttttttTG‑ATTAAAAAAT‑ATAATTAAAT‑TTTTTTTTTTTAAATATCTATATAACGAA‑AACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcg                     <  1:216846/144‑1 (MQ=255)

                                                                                                                                     gt‑ttttttTCG‑ATTTCAAAAT‑ATATTGATTT‑TATTTTTTTTTAATTATGGATATATCTTT‑AACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                 <  2:1190712/149‑1 (MQ=255)

                                                                                                                                     gt‑ttgtttTCG‑ATTTCAAAAT‑ATAATGGAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                 >  2:4136788/1‑149 (MQ=255)

                                                                                                                                     gt‑ttgtttTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                 >  2:1521297/1‑149 (MQ=255)

                                                                                                                                      t‑ttgtttTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                >  2:4897298/1‑149 (MQ=255)

                                                                                                                                          gtttTCT‑ATTTCAAAAT‑ATAATTATTT‑TATTTTTTTTTAAATATCTAGATAACGAT‑CAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa             <  2:1931964/149‑1 (MQ=255)

                                                                                                                                           ttttCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTGTTAt            >  1:4031908/1‑149 (MQ=255)

                                                                                                                                              tCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc         >  2:1842082/1‑149 (MQ=255)

                                                                                                                                               tctttttCAAAAT‑ATAATGAAAT‑TTTTTGTTTTTAAATTTCTATATAACGAA‑CAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc         <  2:2058403/145‑1 (MQ=255)

                                                                                                                                                g‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg       <  2:4206548/149‑1 (MQ=255)

                                                                                                                                                g‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg       >  1:2922927/1‑149 (MQ=255)

                                                                                                                                                g‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg       >  2:5000218/1‑149 (MQ=255)

                                                                                                                                                g‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg       >  2:4999848/1‑149 (MQ=255)

                                                                                                                                                   tttCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt     >  1:2041954/1‑149 (MQ=255)

                                                                                                                                                   ttacAAAAT‑ATAATTAAAT‑TATTTTTTTTTAAATATCTAGATAACGAA‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt     <  1:4648411/146‑1 (MQ=255)

                                                                                                                                                     tCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc   >  2:1712046/1‑149 (MQ=255)

TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCG‑ATTTCAAAAT‑ATAATGAAAT‑TATTTGTTTTTAAATATCGAGATAACGAT‑CACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCG  >  CP009273/2170565‑2170861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A