Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,713 C→A 30.2% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7130CA30.2% 276.7 / 44.8 168intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (26/81);  new base A (35/18);  total (69/99)
Fisher's exact test for biased strand distribution p-value = 7.15e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGC  >  CP009273/2170565‑2170860
                                                                                                                                                    |                                                                                                                                                       
ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑a                                                                                                                                                       >  2:1454405/1‑150 (MQ=255)
 tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑aa                                                                                                                                                      <  1:1616550/150‑1 (MQ=255)
 tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCCGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑aa                                                                                                                                                      <  2:1471639/150‑1 (MQ=255)
 tGGCAGCGGTTTCGACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCCGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑aa                                                                                                                                                      <  2:4203352/150‑1 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAat                                                                                                                                                   <  1:3889965/150‑1 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAat                                                                                                                                                   <  1:2175469/150‑1 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAat                                                                                                                                                   <  1:2455654/150‑1 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAat                                                                                                                                                   <  2:1129397/150‑1 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTA‑aaaaa                                                                                                                                                   >  2:4168486/1‑149 (MQ=255)
     aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAata                                                                                                                                                  <  2:724588/150‑1 (MQ=255)
     aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAata                                                                                                                                                  <  2:724490/150‑1 (MQ=255)
     aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAata                                                                                                                                                  <  2:496386/150‑1 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAatat                                                                                                                                                 <  2:4159198/150‑1 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAAtata                                                                                                                                                <  1:2494121/150‑1 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAAtata                                                                                                                                                <  2:4556260/150‑1 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAAtata                                                                                                                                                <  1:2615354/150‑1 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAAtata                                                                                                                                                <  1:2492629/150‑1 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTC‑CAAAtata                                                                                                                                                >  2:3481898/1‑150 (MQ=255)
        ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTA‑AAAATATaa                                                                                                                                               >  2:349829/1‑150 (MQ=255)
        ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATaa                                                                                                                                               <  1:3503217/150‑1 (MQ=255)
        ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATaa                                                                                                                                               <  1:3505974/150‑1 (MQ=255)
        ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATaa                                                                                                                                               <  2:3382847/150‑1 (MQ=255)
        ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTAGATTTA‑AAAAAATaa                                                                                                                                               >  2:502938/1‑150 (MQ=255)
         gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTA‑AAAATATAAt                                                                                                                                              >  2:1274561/1‑150 (MQ=255)
          tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATg                                                                                                                                             <  2:4153898/150‑1 (MQ=255)
          tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGGGTTTTTTGTTTA‑AAAATATaaat                                                                                                                                             >  1:1226703/1‑148 (MQ=255)
              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAat                                                                                                                                         <  1:4621232/150‑1 (MQ=255)
                 caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTCGATTTA‑AAAATATAATGTAattat                                                                                                                                      >  2:793541/1‑150 (MQ=255)
                  accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAattatt                                                                                                                                     <  2:4173377/150‑1 (MQ=255)
                  accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTA‑AAAATATAAATAAattatt                                                                                                                                     >  2:1658964/1‑150 (MQ=255)
                   ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTC‑AAAATATAATGAAAATAttt                                                                                                                                    >  2:1638920/1‑150 (MQ=255)
                   ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTAGATTTA‑AAAAAAAAATGAAATTAttt                                                                                                                                    >  2:4043897/1‑150 (MQ=255)
                    cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTg                                                                                                                                   <  2:1342413/150‑1 (MQ=255)
                     acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGt                                                                                                                                  <  2:2437922/150‑1 (MQ=255)
                      cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGtt                                                                                                                                 <  2:3283940/150‑1 (MQ=255)
                          cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACATACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTa                                                                                                                             <  2:3546794/150‑1 (MQ=255)
                              gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAa‑ta                                                                                                                        <  2:1098954/150‑1 (MQ=255)
                              gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAa‑ta                                                                                                                        <  1:4622173/150‑1 (MQ=255)
                              gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAa‑ta                                                                                                                        <  1:1408926/150‑1 (MQ=255)
                                   gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCgag                                                                                                                   <  1:552607/150‑1 (MQ=255)
                                     gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGAt                                                                                                                 <  1:337757/150‑1 (MQ=255)
                                       ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATaa                                                                                                               <  1:1272623/150‑1 (MQ=255)
                                       ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATaa                                                                                                               <  1:4175409/150‑1 (MQ=255)
                                        tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAAc                                                                                                              <  2:364280/150‑1 (MQ=255)
                                        tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTA‑AAAAAATAAAGAAAATATTTTTTTTTTAA‑TATAGAGATAAc                                                                                                              >  1:1524314/1‑150 (MQ=255)
                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATACCg                                                                                                             <  1:1986154/150‑1 (MQ=255)
                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACg                                                                                                             <  2:4506432/150‑1 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGAATTC‑AAAATAAAATTAAAATATTTTTTTTTAAA‑TATAGAGATAAAGAt                                                                                                           >  2:2247393/1‑150 (MQ=255)
                                            aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTA‑AAAATATTATTAAAATATTTTTTTTTTAA‑TATAGAGATAACCATc                                                                                                          >  2:3022389/1‑150 (MQ=255)
                                            aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTATTTTTG‑AAAATATAATTTAATTATTTTTTTTTAAA‑TATAGATATaaacata                                                                                                          >  2:3022383/1‑145 (MQ=255)
                                            aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATACCCTGTCGTTTTTTTTGGATTTA‑AAAATATAATTAAAATATTTTTTTTTTAA‑TATATAGATAAAGAta                                                                                                          >  2:4529511/1‑149 (MQ=255)
                                               atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGAGACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATcac‑a                                                                                                      <  1:847443/150‑1 (MQ=255)
                                                 tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑aaa                                                                                                    <  2:9820/150‑1 (MQ=255)
                                                 tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTGGATTTT‑AAAATATAATGAAAATATTTTTTTTTTAA‑TATAGAGATTACGATCAC‑aaa                                                                                                    >  1:3378027/1‑150 (MQ=255)
                                                 agAATGGCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTCTTAAA‑TATCGAGATAACGATCAC‑aaa                                                                                                    <  2:2401356/149‑1 (MQ=255)
                                                   aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑aaaaa                                                                                                  <  2:2921667/150‑1 (MQ=255)
                                                    aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAAc                                                                                                 <  2:3406260/150‑1 (MQ=255)
                                                     tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTC‑AAAATATAATTAAATTATTTGTTTTTTAA‑TATAGTGTTAAAGATCAC‑aaaaaag                                                                                                >  2:2872050/1‑148 (MQ=255)
                                                        cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTA‑AAAATAAAATGAAATTATTTGTTTTTAAA‑TATAGAGATAACGATCAC‑AAAAACGaaa                                                                                             >  2:3440844/1‑148 (MQ=255)
                                                         ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGTTTTC‑ATAAAATAAAGTAAAAATTTTTTTTTTTA‑TATAGTGATAAACAACAC‑AAAAAAGACaa                                                                                            >  1:2102703/1‑150 (MQ=255)
                                                           aaCCGAATAACCGCCGCGCTGTGCGTTTTTATGATTCTGATTTGTCGATACCACTTAATTTTTCATATCCTGTCTTTTTTTTTCTATTTA‑AAAATATAATTAATTTTTTTTTTTTTAAT‑TATCTTTATAACGATAAA‑AAACACGACAata                                                                                          <  2:1483788/150‑1 (MQ=255)
                                                               gCATAACCGCCGCGCTGTGCGTTTTTCTGCATGTGCTTTGTCGATACCACGTACATTTTCTTATCCTGTCGTTTGTTTTCTTTTTC‑AAAATATAATTAAATTATTTGTTTTTAAA‑TATCGAGATAACTATCAC‑AAAAACGACAATATGaa                                                                                      <  2:4171617/150‑1 (MQ=255)
                                                               gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTTTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGaa                                                                                      <  2:2365799/150‑1 (MQ=255)
                                                                cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTTTTTTG‑AAAAAAAAATGAAAATATTTTTTTTTTAA‑AATATAGATAAAAAAAAC‑ACAAAACACAATAAGaaa                                                                                     >  1:1283678/1‑150 (MQ=255)
                                                                  tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTC‑AAAATATAATGTAATTATTTTTTTTTTAA‑TATAGAGATAACGATCAA‑AAAAAAGACAATATGAAAat                                                                                   >  2:4309910/1‑150 (MQ=255)
                                                                  tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTA‑AAAATATAATTAAATTATTTTTTTTTAAA‑AATAGAGATAACCCTAAC‑AAAAACGACAAAATTAAAat                                                                                   >  2:1335857/1‑150 (MQ=255)
                                                                      cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTA‑AAAAAAAAATTAAAATATTTTTTTTTTAA‑AAAATAGGTAACGATAACAAAAAACAACAAAATCAAAattat                                                                                >  1:4352631/1‑150 (MQ=255)
                                                                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTCGAATTA‑AAAATAAAAAGAAATTATTTGTTTTTAAA‑TTTCGAGATAACGATAAA‑AAAAAAGACAATAATAAAaatatta                                                                              >  2:1034708/1‑149 (MQ=255)
                                                                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTC‑AAAATATAATGAAAATATTTTTTTTTTTA‑TATATAGATAAAGATAAC‑AAAAAAGAAAAAATGAAAattatta                                                                              >  2:4081506/1‑149 (MQ=255)
                                                                          gcgcTGTGCGTTTTTCAGCATCTGCTTTGTCGATACCACTTACATTTTAATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAATTTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCgag                                                                           <  2:2236514/150‑1 (MQ=255)
                                                                                     tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGTTTTA‑AAAAAATAAATAAAATAATTTTTTTTTAA‑TATAGATATAAACAAAAC‑AAAAAAAACAAAATTAAAAATATTTGAGGAG‑TTTAAGGc                                                               >  1:284405/1‑150 (MQ=255)
                                                                                       ttCAGCATCTGCTTTGTCTATACCACTTACATTTTCATATCCTGTCTTTTGTTTTCTATTTC‑AAAATATAATTAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCaa                                                             <  2:2158611/150‑1 (MQ=255)
                                                                                       ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTA‑AAAAAATAATGAAATTATTTTTTTTTTAA‑TATCGAGATAAAGATAAC‑AAAAACGAAAATATTAAAATTATTAGATGAG‑TGAAATGCaa                                                             >  2:3579749/1‑150 (MQ=255)
                                                                                        tCAGAATCTTTTTTGTCTAAACCACTTAAATTTAAATATCCTGTTTTTTTTTTTCTATTAA‑AAAATATAATTAATTTTTTTTTTTTAAAT‑TATCTATAAAACTAAAAA‑AAACACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCaaa                                                            <  1:3267233/150‑1 (MQ=255)
                                                                                          aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTG‑AAAATATAATGAAATTATTTTTTTTTTAA‑TATAGATATAAAGATAAC‑AAAAAAAACAAAAATAAAATTATTTGAGGGG‑TGTAAGGGaaaaa                                                          >  2:2009255/1‑150 (MQ=255)
                                                                                              tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTA‑AAAATATAATGAAATTATTTTTTTTTAAA‑TATCTAGATAACCATAAC‑AAAAAAGACAAAATTAAAATTATTCGAGGAT‑TGAAATGCaaaaaaaag                                                      >  2:401805/1‑148 (MQ=255)
                                                                                                  cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTC‑AAAATATAATGAAATTATTTTTTTTTAAA‑TATCGAGATAAAGATAAC‑AAAAAAGACAATATTAAAATTATTGGTGGAGTTTAAGGGCAAAAAAACgcca                                                   >  2:2708306/1‑147 (MQ=255)
                                                                                                     ggtctacaCCACGTACATTTACATATCCTGTCGTTTGTTTTCTATTAC‑AAAATATAATGAAATTATTTTTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTccc                                               <  2:2342232/143‑1 (MQ=255)
                                                                                                     ggacGATCCCCCGTACATTTTCATACCCCGCCGTTTGTGTTCGATTAC‑AAAATATAATGAAATTTATTGTTTTTAAA‑TATCGAGATAACGACCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTccc                                               <  2:2947096/147‑1 (MQ=255)
                                                                                                          aaaCCACTTACTTTTTAATAACCTGTCGTTTGTTTTCGATTTA‑AAAATATAATCAATTTATTTTTTTTTAAA‑TATCGAGATAACTATCAA‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAg                                          <  2:2471852/148‑1 (MQ=255)
                                                                                                           tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTA‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATACCGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAgg                                         <  2:3107848/150‑1 (MQ=255)
                                                                                                            aCCACGTACAGTTTCATATCCTGTCGTTTGTTTTAGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATAGAGATAACGATAAC‑AAAAAACAAAAAAATAAAAATATTCGAGGGG‑TGAAATGGAAAAAAAAGGCCACCCGaaatgg                                        >  2:4771282/1‑145 (MQ=255)
                                                                                                              cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                      <  2:352624/150‑1 (MQ=255)
                                                                                                                   aaaTTTTAATTTCTTGTC‑TTTTTTTTCATTTTAAAAAATATAAT‑AAATTTTTTTTTTTAAAATTACCTAGAAAACCATCAA‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGt                                 <  1:3722270/148‑1 (MQ=255)
                                                                                                                          cATATCCTTTCGTTTGTTTTCTATTAC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAA‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAg                          <  2:3803089/150‑1 (MQ=255)
                                                                                                                              tCCTGTCGTGTGTTTTAGATTTA‑AAAAAAAAAAGAAAAAATTTTTTTTTAAA‑TATCGAGATAACCATCAC‑AAAAAACAAAAAAATAAAAATAATAGAGGAG‑GGAAAGGCAAAAAAACAGCCACCCCAAAGGGCACCCGTAGCAAAATgcg                      >  2:533599/1‑150 (MQ=255)
                                                                                                                                 tGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTTTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAAAGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                   >  2:4230851/1‑150 (MQ=255)
                                                                                                                                  gTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                  >  2:642447/1‑150 (MQ=255)
                                                                                                                                   tCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AATAACGACAATATGAATATTATTCGAGGTG‑TGTAAGGCATATTTTCGGCCTCCCGATTGGGAAGCGGTAGCAAAGTGCGCgtgt                 >  2:2780553/1‑150 (MQ=255)
                                                                                                                                      ttttttttCGATTTC‑AAATTATAATTAATTTTTTTTTTTTTAAA‑TATCGAGATAACTAACAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt              <  1:703983/150‑1 (MQ=255)
                                                                                                                                      tttgtttTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGATATAACGATCAA‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt              <  2:2581817/150‑1 (MQ=255)
                                                                                                                                      tttgtttTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCTAAGTGCGCGTGtttt              >  1:1114436/1‑150 (MQ=255)
                                                                                                                                       tttttttCTATTTC‑AAAATATAATTAAATTATTTGTTTTTAAA‑TATCGATATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt             <  1:297519/147‑1 (MQ=255)
                                                                                                                                       ttgtttTCTATTAC‑AAAATATAATTAATTTTTTTGTTTTTAAA‑TATCGATATAACTATCAA‑AAACACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt             <  1:298368/150‑1 (MQ=255)
                                                                                                                                        tgtttTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa            >  2:1959418/1‑150 (MQ=255)
                                                                                                                                           tttCGATTTC‑AAAATATAATGAAATTTTTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACTGCCTCCCGATAGGGATGCCGTAGCACAGTGCGCGTGTTTTTATGc         <  1:3569600/150‑1 (MQ=255)
                                                                                                                                            ttCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc        >  1:3965806/1‑150 (MQ=255)
                                                                                                                                            ttCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc        >  2:309875/1‑150 (MQ=255)
                                                                                                                                               taTTTA‑AAAAAATAATTAAATTATTTTTTTTTAAT‑TATCTTTATAACTAAAAA‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa     <  1:1757167/149‑1 (MQ=255)
                                                                                                                                                  ttC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc  >  2:325460/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTC‑AAAATATAATGAAATTATTTGTTTTTAAA‑TATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAG‑TGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGC  >  CP009273/2170565‑2170860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A