Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F26 I0 R1 94 331.7 11075696 93.7% 10377927 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,713 C→A 21.0% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7130CA21.0% 459.9 / 19.3 214intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (47/106);  new base A (37/14);  total (94/120)
Fisher's exact test for biased strand distribution p-value = 2.03e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  CP009273/2170564‑2170862
                                                                                                                                                      |                                                                                                                                                     
gTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTc                                                                                                                                                       <  1:5231273/150‑1 (MQ=255)
 ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCa                                                                                                                                                      <  1:1837872/150‑1 (MQ=255)
   ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCTATTTCaaa                                                                                                                                                    >  2:3035529/1‑150 (MQ=255)
    gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCaaaa                                                                                                                                                   <  1:2508406/150‑1 (MQ=255)
    gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCaaaa                                                                                                                                                   <  2:1620602/150‑1 (MQ=255)
     cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAat                                                                                                                                                  <  1:4136557/150‑1 (MQ=255)
     cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAat                                                                                                                                                  <  1:3929804/150‑1 (MQ=255)
      aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAata                                                                                                                                                 <  2:2898402/150‑1 (MQ=255)
      aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAata                                                                                                                                                 <  1:4609404/150‑1 (MQ=255)
      aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAata                                                                                                                                                 <  2:1883823/150‑1 (MQ=255)
      aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTAAAAata                                                                                                                                                 >  2:5231073/1‑150 (MQ=255)
      aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTTGTTTTTaaaaaa                                                                                                                                                 >  1:956429/1‑148 (MQ=255)
      aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGATTTTaaaaaa                                                                                                                                                 >  1:1966044/1‑148 (MQ=255)
         ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATaa                                                                                                                                              <  2:4609871/150‑1 (MQ=255)
          gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAAt                                                                                                                                             <  2:171184/150‑1 (MQ=255)
          gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAAt                                                                                                                                             <  1:1896052/150‑1 (MQ=255)
          gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAAt                                                                                                                                             <  2:1974340/150‑1 (MQ=255)
           tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATg                                                                                                                                            <  2:4396549/150‑1 (MQ=255)
             tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                          <  2:195097/150‑1 (MQ=255)
              cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                         <  1:2563418/150‑1 (MQ=255)
               tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                        <  2:311195/150‑1 (MQ=255)
               tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAat                                                                                                                                        <  1:5114587/150‑1 (MQ=255)
                accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTAAAAATAAAATGaaaat                                                                                                                                       >  2:4694890/1‑148 (MQ=255)
                   accacTTGCATCGTTTCGAGGTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTCAAAATATAAATAAattatt                                                                                                                                    >  2:549064/1‑150 (MQ=255)
                   accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTGGATTTAAAAAAAAAAATAAaatatt                                                                                                                                    >  1:4485764/1‑150 (MQ=255)
                   accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATTAAattatt                                                                                                                                    >  2:1252666/1‑150 (MQ=255)
                     cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTg                                                                                                                                  <  1:1181620/150‑1 (MQ=255)
                     cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTg                                                                                                                                  <  1:2042808/150‑1 (MQ=255)
                      acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                                 <  1:2623744/150‑1 (MQ=255)
                       cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                <  2:2641319/150‑1 (MQ=255)
                       cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCGATTTAAAAAAATAATTTAAATAtttttt                                                                                                                                >  1:1369848/1‑150 (MQ=255)
                        ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                               <  2:4309435/150‑1 (MQ=255)
                        ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                               <  1:2916438/150‑1 (MQ=255)
                         tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                              <  1:2083433/150‑1 (MQ=255)
                          gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGttttt                                                                                                                             <  2:282762/150‑1 (MQ=255)
                           cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                            <  1:4375446/150‑1 (MQ=255)
                            aTCGTTTCGAGATTGTGAATATTGAATGCAGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTAAAAAAATAATGAAAATATTTTTTTTTaa                                                                                                                           >  2:2075386/1‑150 (MQ=255)
                             tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaaa                                                                                                                          <  1:1904588/150‑1 (MQ=255)
                             tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCGATTTCAAAATATAATGAAAATAATTTTTTTTaaa                                                                                                                          >  1:1741365/1‑150 (MQ=255)
                               gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAata                                                                                                                        <  2:3869312/150‑1 (MQ=255)
                               gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTATAGTTAAAAAAAAAATTAAAATATTTTTTTTTaaaaa                                                                                                                        >  1:5375816/1‑148 (MQ=255)
                               gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGGTTTGGATTTCAAAAAAAAAATAAAAAATTTTTTTTTTAata                                                                                                                        >  1:1472005/1‑150 (MQ=255)
                                tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                       >  2:3685752/1‑150 (MQ=255)
                                tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                       <  2:2698901/150‑1 (MQ=255)
                                tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                       <  2:3540870/150‑1 (MQ=255)
                                 ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                      <  1:3510800/150‑1 (MQ=255)
                                    gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                                   >  2:3903056/1‑150 (MQ=255)
                                    gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgag                                                                                                                   <  1:3694327/150‑1 (MQ=255)
                                     agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGTAAATATTTTTTTTTTAATATAgata                                                                                                                  >  2:727723/1‑148 (MQ=255)
                                     agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGTATTTAAAAAAAAAAATAAAAAAATTTTTTTTAAATAAAgaga                                                                                                                  >  1:2626910/1‑150 (MQ=255)
                                      gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGAt                                                                                                                 >  2:351946/1‑150 (MQ=255)
                                      gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                 <  2:5207346/150‑1 (MQ=255)
                                          gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTAGATTTAAAAAAATAATGAAATTATTTTTTTTTTAAAATATAGATaaac                                                                                                             >  1:2269375/1‑148 (MQ=255)
                                             aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAGATTTAAAAATATAATTAAATTATTTGTTTTTAAATATAGAGATAAAGATc                                                                                                          >  2:4059908/1‑150 (MQ=255)
                                             aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                          <  2:1676653/150‑1 (MQ=255)
                                                atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                       <  1:2230691/150‑1 (MQ=255)
                                                    aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GGGTTTTAGAATTCAAAAAAAAAAGAAAAAATATTTTTTTAAATAAAGAGATAACCATAACaaaaa                                                                                                   >  1:2888698/1‑150 (MQ=255)
                                                      tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACg                                                                                                 <  2:1818957/150‑1 (MQ=255)
                                                        ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTGTTTT‑GATTTAAAAAAATAAATAAATTATTTTTTTTTTAATTTAGATATAAAGATaaaaaaaaaaaa                                                                                               >  1:3410832/1‑146 (MQ=255)
                                                        ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTGGATTTAAAAATATAAAGAAAATATTTTTTTTTTAAAATAGAGATAAAGAACACaaaaaacac                                                                                               >  2:1553060/1‑146 (MQ=255)
                                                               cGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTGGATTTTAAAATATAATTAAAATATTTTTTTTTTAATATAGAGATAAAAATAAAAAAAAAAACAaaattt                                                                                        >  1:1618820/1‑148 (MQ=255)
                                                                  aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACTTAAATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGaaaa                                                                                     <  2:2239353/150‑1 (MQ=255)
                                                                   tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGTGATAACGATCACAAAAACGACAATATGAAAat                                                                                    <  2:3213467/150‑1 (MQ=255)
                                                                    acccgccgCGGTGTGCTTTTTTCAGCATCTGCTTTGTCTATACCACTTAAATTTTAATATCCTTTCTT‑TTTTTTTCTTTTTCAAAATATAATTAATTTTTTTTTTTTTAAATATCGAGATAACTATCAAAAAAACGACAATATGAAAatt                                                                                   <  1:3002752/148‑1 (MQ=255)
                                                                      ccgccgCGCTGGGCGTTGTTCACAATCTGCTTTGTCGATACCACTTAAATTTTCATACCCTGTCGT‑TTTTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAattat                                                                                 <  1:1845748/150‑1 (MQ=255)
                                                                       cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTAAAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt                                                                                <  2:3823816/150‑1 (MQ=255)
                                                                        gccgcGCTGTGAGTTGTTCAGCATGTGCTTTGTCTATACCACTTACATTTTCATATCCTGTCGT‑TTGTTTTCTATTTCAAAATATAATTAAATTTTTTTTTTTTAAATATCGAGATAACGATCAAAAAAACGACAATATGAAAATTATTc                                                                               <  2:4407338/150‑1 (MQ=255)
                                                                         ccgcGCTGTGCTTTGTTCCGAATCGGCTTTGTCTATACCACGTACATTTTCATAACCTGTCGT‑TTTTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGAACACAAACACGACAATATGAAAATTATTCg                                                                              <  2:5180038/150‑1 (MQ=255)
                                                                         ccgcGCTGTGCGTTTTTCAGCATCTGCTTTGGCGATACCACGTACATTTTCATATCCTGTCTT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCg                                                                              <  2:5180739/150‑1 (MQ=255)
                                                                          cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGATTTAAAAAAATAATTAAATAATTTTTTTTTAAATAAAGAGAGAAACAAAACAAAAAAAACAAAAAGAAAaataatagc                                                                             >  2:948574/1‑145 (MQ=255)
                                                                              cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGAATTAAAAAAAAAATGTAAATATTTTTTTTTTAAAAAAGATAAAACCATAACAAAAACAAAAAAAATAAAAATATTCgatgag                                                                         >  2:17348/1‑150 (MQ=255)
                                                                                  ggtttgttCAGCATCGGATTTGTCGATACCACCTACATTTTCATATCCTGTCGT‑GTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaa                                                                     <  2:2450769/147‑1 (MQ=255)
                                                                                      tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCGATTTCAAAAAATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAAAGACAAAATGAAAATTATTCGAGGAGTGAAAGGc                                                                 >  2:4891340/1‑150 (MQ=255)
                                                                                       gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAAAGACAATATTAAAATTATTAGAGGAGTGAAAGGCa                                                                >  2:866031/1‑150 (MQ=255)
                                                                                            gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaa                                                           <  2:4900168/150‑1 (MQ=255)
                                                                                                             aCCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                          <  2:3610167/150‑1 (MQ=255)
                                                                                                                ccctaaaTTTTAATATCCTGTGTT‑TTTTTTCCTATTACAAAATATAATTAATTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                       <  2:956429/144‑1 (MQ=255)
                                                                                                                  ttaatttttCATATCGTGTCTT‑TTTTTTTCTTTTTAAAAATATATTTAATTTTTTTTTTTTTAAATATCTATAAAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                     <  1:226387/145‑1 (MQ=255)
                                                                                                                  gTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                     <  2:4534478/150‑1 (MQ=255)
                                                                                                                     cATTTTCATATCCTGTCGT‑GGGTTTTGGATTTCAAAATATAATGTAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACCATATGAAAAGTATTGGAGGAGTGCAAGGCCAAAAAACGGCCTCCCGCGAGGGAAGCCGTa                                  >  2:4519027/1‑150 (MQ=255)
                                                                                                                     aaTTTTCAAACCCTGTCGT‑TTTTTTACGAATAAAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  1:4522585/149‑1 (MQ=255)
                                                                                                                        tttCATATCCGGTCGT‑TTGTTTTCTATTTCAAAATATAATGAATTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCa                               <  2:4944734/150‑1 (MQ=255)
                                                                                                                              atCCTGTCGT‑TTGTTTTCGATTTAAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                         <  2:796783/150‑1 (MQ=255)
                                                                                                                                ccTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGTAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTATCAAAGTgcgc                       >  2:1370904/1‑150 (MQ=255)
                                                                                                                                    tCGT‑TTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                   <  2:2623540/150‑1 (MQ=255)
                                                                                                                                     cGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                  >  2:2293306/1‑150 (MQ=255)
                                                                                                                                       t‑ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt                >  1:207585/1‑150 (MQ=255)
                                                                                                                                         ttgtttTCGTTTTCAAAATATAATGAAATTTTTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt               <  1:5534083/150‑1 (MQ=255)
                                                                                                                                         ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt               >  1:1507961/1‑150 (MQ=255)
                                                                                                                                          tgtttTCTATTTCAAAATATAATGAATTTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa              <  2:2228871/150‑1 (MQ=255)
                                                                                                                                          tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAATCGGCCTCCCGATCGGGAAGCCGTAGCAACGTGCGCGTGTTTTTa              >  2:1474376/1‑150 (MQ=255)
                                                                                                                                          tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa              >  2:5481987/1‑150 (MQ=255)
                                                                                                                                           gtttTCGATTTCAAAATATAATGAATTTATTTGTTTTTAATTATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  2:1392851/150‑1 (MQ=255)
                                                                                                                                            ttttCTTTTTAAAAATATAATTAAATTATTTTTTTTTAAATATCTATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            <  1:866031/150‑1 (MQ=255)
                                                                                                                                            ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            <  2:4455534/150‑1 (MQ=255)
                                                                                                                                              ttCTATTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc          <  1:5132615/150‑1 (MQ=255)
                                                                                                                                              ttCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc          <  2:3587016/150‑1 (MQ=255)
                                                                                                                                                cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg        >  1:657973/1‑150 (MQ=255)
                                                                                                                                                  aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt      >  2:2798032/1‑150 (MQ=255)
                                                                                                                                                      cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  2:1445046/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  CP009273/2170564‑2170862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A