Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,713 C→A 32.2% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7130CA32.2% 292.3 / 36.3 190intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (35/78);  new base A (38/25);  total (87/103)
Fisher's exact test for biased strand distribution p-value = 2.21e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

TGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  CP009273/2170566‑2170862
                                                                                                                                                    |                                                                                                                                                     
tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCaa                                                                                                                                                     <  1:3121849/150‑1 (MQ=255)
  gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCaaaa                                                                                                                                                   <  1:3815552/150‑1 (MQ=255)
  gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCaaaa                                                                                                                                                   <  2:2160898/150‑1 (MQ=255)
   cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAat                                                                                                                                                  <  1:3429479/150‑1 (MQ=255)
   cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAat                                                                                                                                                  <  1:2077791/150‑1 (MQ=255)
    aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAata                                                                                                                                                 <  2:70003/150‑1 (MQ=255)
    aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCGATTTAaaaaaa                                                                                                                                                 >  2:2783414/1‑148 (MQ=255)
     gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCTAAatat                                                                                                                                                <  1:134887/150‑1 (MQ=255)
     gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAatat                                                                                                                                                <  1:3779200/150‑1 (MQ=255)
     gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAatat                                                                                                                                                <  2:1418018/150‑1 (MQ=255)
     ctggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAatat                                                                                                                                                <  1:4354260/148‑1 (MQ=255)
      cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAAtata                                                                                                                                               <  2:3208805/150‑1 (MQ=255)
        gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAAt                                                                                                                                             <  2:4275174/150‑1 (MQ=255)
         tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATg                                                                                                                                            <  2:494736/150‑1 (MQ=255)
         tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGT‑GTGGTTTTGATTTCAAAATATAATg                                                                                                                                            >  1:892431/1‑150 (MQ=255)
           tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                          <  1:4232469/150‑1 (MQ=255)
           tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                          <  1:1231072/150‑1 (MQ=255)
              accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAAAATAATGaaaat                                                                                                                                       >  2:1698041/1‑148 (MQ=255)
                caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAaataa                                                                                                                                     >  2:2371169/1‑149 (MQ=255)
                 accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    <  1:3021804/150‑1 (MQ=255)
                 accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGATTTAAAAATATTATTAAaatatt                                                                                                                                    >  1:622489/1‑150 (MQ=255)
                   cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTg                                                                                                                                  <  2:1910374/150‑1 (MQ=255)
                   cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTg                                                                                                                                  <  1:1463304/150‑1 (MQ=255)
                     cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGAATTTCAAAAATTATTAAAAtttttttt                                                                                                                                 >  1:3720255/1‑148 (MQ=255)
                      ttGCATCGTTTAGAGATTGTTAATATTGAATGCCTGAACCTCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                               <  2:1162512/150‑1 (MQ=255)
                      ttGAATCGTTTCGAGATTGTGAATATTGAATTCCGTAACCGCATAACCGCCGAGCTGTTCGTTGTTCAGCATCTGCTTTTTCGATACCACGTTCATTTTCATATCCTGTCGT‑TTGTTTTAGATTTCAAAATATAATTAAATTATTTGttt                                                                                                                               <  1:2263480/150‑1 (MQ=255)
                       tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTCAAAATATAATTAAATTAtttttttt                                                                                                                              >  2:3879394/1‑150 (MQ=255)
                       tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTTTATTTTAAAAAATAAATAAATAAtttttttt                                                                                                                              >  1:3319952/1‑150 (MQ=255)
                        gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTGGATTTCAAAAAATAAATAAATTATtttttttt                                                                                                                             >  2:1119086/1‑150 (MQ=255)
                             gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTCGATTTCAAAATATAATTAAAATATTTTTTTTTAAata                                                                                                                        >  2:1772866/1‑150 (MQ=255)
                             gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCTATTTCAAAATATAATGAAATTTTTTTTTTTTTAata                                                                                                                        >  1:1724997/1‑150 (MQ=255)
                               ttCGAGTTTGTTAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTTGTTTGTCGATACCACGTACATTTTCATATCCTTTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                      <  2:1756270/150‑1 (MQ=255)
                               ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                      <  1:3149981/150‑1 (MQ=255)
                               ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                      <  1:2308078/150‑1 (MQ=255)
                               gtCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                      <  1:1556197/149‑1 (MQ=255)
                                   agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgaga                                                                                                                  <  2:3317504/150‑1 (MQ=255)
                                   agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTAAAAAAATAATGAAATTATTTTTTTTTAAATATAgaga                                                                                                                  >  2:4198817/1‑150 (MQ=255)
                                      ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAGAATTGAAAAAAAAAATAAATTATTTTTTTTTAAATATAGAGaaaa                                                                                                               >  2:3399254/1‑150 (MQ=255)
                                       tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                              <  2:3720598/150‑1 (MQ=255)
                                        gtgAATATTGCATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                             <  1:4166594/150‑1 (MQ=255)
                                        gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTTGATTTAAAAAAATAAATTAATTAATTTTTTTTAATTATAGAGATaaag                                                                                                             >  1:3135745/1‑148 (MQ=255)
                                        gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATTAAAATATTTTTTTTTAAATATAGAGATaaag                                                                                                             >  2:1403288/1‑148 (MQ=255)
                                          gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGTTTTGAAAAAATAAATAAAATATTTTTTTTTTTATATAGTGGTAACGAt                                                                                                           >  1:3716891/1‑150 (MQ=255)
                                           aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                          <  2:3172288/150‑1 (MQ=255)
                                           aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                          <  2:2250553/150‑1 (MQ=255)
                                            atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGATATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                         <  2:3479080/150‑1 (MQ=255)
                                             tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGATTTAAAAATAAAATGAAATTATTTTTTTTTTAATAAAGATATaaacaacac                                                                                                        >  1:331313/1‑143 (MQ=255)
                                                    tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACg                                                                                                 <  2:3236417/150‑1 (MQ=255)
                                                    tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTATATTTATAAATAAAAATAAAATAATTTTTTTTTTATATAGAGTTACAGAGAACAAAAAca                                                                                                 >  1:4036753/1‑149 (MQ=255)
                                                    tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGATTTAAAAATATAATTTAATTATTTTTTTTTTAATATAGAGATAAATATaaaaaaaaag                                                                                                 >  2:639737/1‑148 (MQ=255)
                                                     gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa                                                                                                <  2:2101935/150‑1 (MQ=255)
                                                            ccGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGTTTTTTAAAAAATAATTAAAATATTTTTTTTTTAATATAGTGATAAACATAAAAAAAACAACAaaatt                                                                                         >  1:573695/1‑149 (MQ=255)
                                                            ccGAATAACCGCCGGGGTGTGGGTTTTTCAGAATGTGTTTTTTCTATACAACTTAATTTTTCTTATCGTGTGGT‑TTTTTTTCTTTTTAAAAATATATTTAATTTTTTTTTTTTTAAATTTGGATATAACTATCACAAAAACGACAATATg                                                                                         <  2:4103777/150‑1 (MQ=255)
                                                                 tAACCGCCGCGCTGTGGGTTTTTCAGCATTTGCTTTGTCGATACCCCTTAAATTTTCATATCCTGTGTT‑TTGTTTTCGATTAAAAAATATATTTAATTTTTTTTTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAat                                                                                    <  2:2181803/150‑1 (MQ=255)
                                                                  aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGGTTTAAAAAAAAAATTTAATTATTTTTTTTTAAATATAGAGGTTACGAACACAAAAAAGACAAAATGagaaat                                                                                   >  1:3572728/1‑148 (MQ=255)
                                                                  aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGATTTAAAAAAATAAAGAAAATATTTTTTTTTTTATATATGGATAAAAATAACAAAAAACACAAAATGtaaaat                                                                                   >  1:1381501/1‑148 (MQ=255)
                                                                  aaCCGCCGCGCTGTGCGTTGTCCAGCATCTGCTTTGTCTATACCACGTACTTTTAAATATCCTTTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatt                                                                                   <  2:4335389/150‑1 (MQ=255)
                                                                   aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGGTTTGGATTTAAAAATATAATTAAAATATTTTTTTTTTAAAATAGAGATGACGATCACAAAAACAACAAAATGAAAaata                                                                                  >  1:2089058/1‑150 (MQ=255)
                                                                      gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                               <  2:1423091/150‑1 (MQ=255)
                                                                            cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTGGATTTAAAAATATAATTAAAATATTTTTTTTTTAAAATAGCGATAAAGATAAAAAAAAACAAAAAATTAAAAATATTAgaggag                                                                         >  1:2512430/1‑150 (MQ=255)
                                                                            cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GGGTTTTTTTTTTTTAAATATAATTTAAATATTTTTTTTTTTATATTAAGATAAAGATCAAAAAAAAAACAAAAATAAAaatattattggtg                                                                         >  1:2944850/1‑143 (MQ=255)
                                                                             tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTTTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAAAGATCACAAAAACGACAATATTTAAAATATTAGATGAGt                                                                        >  2:1628544/1‑150 (MQ=255)
                                                                             tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTAGATTTAAAAATAAAATTAAATTATTTTTTTTTTAAAAAATATATAAACATAACAAAAAAAAAAAAATTAAAAATATTAGAGGAGt                                                                        >  1:337299/1‑150 (MQ=255)
                                                                              gtgCGTTGTTCAGCATCTGCTTGGTCGATACCACGTACATTTTCATATCCGGTCGT‑TTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTg                                                                       <  2:1351616/150‑1 (MQ=255)
                                                                               tgCGTTGTTCAGCATCTGCTTGGTCGATACCACGTACATTTTCATATCCTGGCGT‑TTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGa                                                                      <  2:1010578/150‑1 (MQ=255)
                                                                                gCGTTGTTCAGCATCTGCTTTGTCGATACCACGTAAATTTTAATATCCTGTCGT‑TTTTTTTCGATTAAAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaa                                                                     <  2:2411795/150‑1 (MQ=255)
                                                                                     gttCAGCATCTGCTTTGTCTATACCCCGTAAATTTTCATATCCTGTCGT‑TTGTTTTCTATTTCAAAATATAATGAATTTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCa                                                                <  2:3556273/150‑1 (MQ=255)
                                                                                     gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑GTTTTTTAGATTTAAAAATATAATTAAAATAATTTTTTTTAAATATCGAGATAAACAAAAAAAAAAAGACAAAATGAAAATTATTAGAGGGGTGTAAGGCa                                                                >  2:1695729/1‑150 (MQ=255)
                                                                                          tcATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCTT‑TTGTTTTCTATTACAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaa                                                           <  2:2997888/149‑1 (MQ=255)
                                                                                               tgttttGTCGATCCCCCGTAAATTTTCATATCCTGTGTT‑TTTTTTTCTTTTAAAAAATATAATTAATTTTTTTTTTTTTAAATATCGATATAACGAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGc                                                      <  1:3890938/147‑1 (MQ=255)
                                                                                                       cGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTAGATTTAAAAATATAATTAAATTATTTTTTTTTTAATATCGAGATAAAGATAACAAAAAAGACAATATTAAAATTATTGGAGGAGTGTAAGGGAAAAAAAAGGCCTCCCGaa                                              >  2:3568260/1‑149 (MQ=255)
                                                                                                       cGATACCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATCGAGTTAACGATCACAAAAACGACACTATGAAAATTATTAGAGGGGTGAAAGGCCAAAAAACCGCCACCCGag                                              >  2:1933523/1‑149 (MQ=255)
                                                                                                          tACCACTTAAATTTTCATATCGTGTCTT‑TTTTTTTCTATTAAAAAATATAATAAAATTATTTTTTTTTAATTATCTATATAACTATCAAAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                           <  1:3477800/150‑1 (MQ=255)
                                                                                                           aCCACGTACATTTTCATATCCTGTCGT‑TTTTTTTGGGTTTAAAAATATAATGAAATTATTTTTTTTTTAATATAGAGATAAAATTAAAAAAAACGAAAATATTAAAATTATTAGTGGGGTGGATTGTCAAAAAAAGGCCGGCCGAGAggg                                          >  2:3989717/1‑150 (MQ=255)
                                                                                                           aCCACGTACATTTTCATATCCTGTCGT‑GTGTTTTTGATTTGAAAATATAATTAAAATATTTTTTTTTTAATTTAGAGTTTACGATAACCAAAACGACAATATTAAAATTTTTAGGGGGGGGTAATGGAAAAAAAAGGCCTGCCGCTTggg                                          >  2:4163751/1‑150 (MQ=255)
                                                                                                           aCCACGTACATTTTCATATCCTGTCGT‑GTGTTTTGGTTTTGAAAAAATAATTTAAATATTTTTTTTTTAATATGGAGATTAACATGACAAAAAAGACCAAATTAAAATTATTTGGGGGGGGTAATGCCAAAAAAAGGCCGCCCGGTTggg                                          >  2:571123/1‑150 (MQ=255)
                                                                                                           aCCACGTACATTTTCATATCCTGTCGT‑GTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTATATATCGAGGTAAAGATCCCAAAAACGACAATATGTAAATTATTGGAGGAGTGGAAGGCAAAAAAAAAGCCACCCGCGAggg                                          >  2:2739579/1‑150 (MQ=255)
                                                                                                             cACTTACTTTTTCTTATCTTGTCTT‑TTTTTTTCTTTTTAAAAATATAATTAATTTTTTTTTTTTTAAATATCTTGATACCGAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                        <  1:1015078/150‑1 (MQ=255)
                                                                                                             cACGTACATTTTAATAACCTGTCTT‑TTTTTTTATATTTAAAAATATAATTAATTTATTTTTTTTTAAATATCTATATAACTATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                        <  1:323275/150‑1 (MQ=255)
                                                                                                               cGTACATTTTCATATCCTGTCGTGTTGTTTT‑GATTTAAAAATAAAATGAAAATATTTTTTTTTTAAAATAGAGATAACGATAAAAAAAAACACCAAAATAAAATTATTAGGGGTGTGGAAGGCAAAAAAAACGCCACCCCAGAGGGGAGc                                      >  2:780183/1‑150 (MQ=255)
                                                                                                                gTACATTTTAAGACCCTGTCTT‑TTGTTTTCGATTCAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                     <  2:2212713/150‑1 (MQ=255)
                                                                                                                gTAAATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                     <  2:258000/150‑1 (MQ=255)
                                                                                                                   cATTTTCATATCCTGTCGT‑GTGTTTTGGATTTACAAAAAAAATTAAAATATTTTTTTTTTAATTTAGAGATAACGATCAAAAAAAATACAATATTAAAATTATTAGTGGGGTTTAAAGGAAAAAAAAAGCCGCCCCCTAGGGGCGCCgga                                  >  2:1187951/1‑148 (MQ=255)
                                                                                                                       ttCATACCCTTTCTT‑TTTTTTTCTTTTTAAAAATATAATTAAATTTTTTTTTTTTAAATATCTATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaa                              <  1:272193/150‑1 (MQ=255)
                                                                                                                           tatCCTGTGGT‑TTTTTTTCTTTTAAAAAATATATTTAATTTTTTTTTTTTAAAATATCGTTATACCCAAAACAACAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                          <  1:1209436/150‑1 (MQ=255)
                                                                                                                                   ctt‑ttgtttTCTATTAAAAAATATAATTAATTTATTTTTTTTTAAATATCGAGATAACGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                  <  2:4041017/148‑1 (MQ=255)
                                                                                                                                   cGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                  >  1:2076723/1‑150 (MQ=255)
                                                                                                                                    gt‑ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                 >  1:945345/1‑150 (MQ=255)
                                                                                                                                    gt‑ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                 >  1:4212708/1‑150 (MQ=255)
                                                                                                                                       ttgtttTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt               <  2:3629053/150‑1 (MQ=255)
                                                                                                                                        tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa              >  2:3130310/1‑150 (MQ=255)
                                                                                                                                         gtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             >  2:1259770/1‑150 (MQ=255)
                                                                                                                                            ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAGATTATTGGTGGAGTGTAAGGCAATAAAACGGCGTCGGGATGGGGTAGCCGTAGCTAGGTGCGGGGGtttgtttggg          >  1:2194285/1‑143 (MQ=255)
                                                                                                                                            ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAACGTGCGCGTGTTTTTATGcc          >  1:1704703/1‑150 (MQ=255)
                                                                                                                                             tCGATTAAAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg         <  2:2664572/150‑1 (MQ=255)
                                                                                                                                               gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa       <  2:895190/150‑1 (MQ=255)
                                                                                                                                                   tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   >  2:1532058/1‑150 (MQ=255)
                                                                                                                                                    cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  2:3778258/1‑150 (MQ=255)
                                                                                                                                                    cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  2:1197638/1‑150 (MQ=255)
                                                                                                                                                    cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  1:3516482/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGT‑TTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  CP009273/2170566‑2170862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A