Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F5 I0 R1 92 232.7 8849178 85.5% 7566047 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,713 C→A 25.5% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7130CA25.5% 286.8 / 27.3 164intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (27/78);  new base A (33/11);  total (75/89)
Fisher's exact test for biased strand distribution p-value = 3.81e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCG  >  CP009273/2170565‑2170861
                                                                                                                                                    |                                                                                                                                                       
ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTc                                                                                                                                                         <  2:3136857/149‑1 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                     <  1:3814247/149‑1 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTCCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                     <  2:2624963/149‑1 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGGGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAata                                                                                                                                                   >  2:3918291/1‑149 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTATATTTAAAAata                                                                                                                                                   >  2:150929/1‑149 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAaaaaaa                                                                                                                                                   >  2:1264095/1‑147 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAata                                                                                                                                                   >  2:151059/1‑149 (MQ=255)
          tttCTACCACCACTTTCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCATCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAt                                                                                                                                              <  2:1915855/149‑1 (MQ=255)
          tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAt                                                                                                                                              <  2:1917281/149‑1 (MQ=255)
           ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATg                                                                                                                                             <  2:1365078/149‑1 (MQ=255)
            tcgaccaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGa                                                                                                                                            <  1:2721691/146‑1 (MQ=255)
             cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGaa                                                                                                                                           <  1:3624646/149‑1 (MQ=255)
              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGaaa                                                                                                                                          <  2:2642790/149‑1 (MQ=255)
               accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAat                                                                                                                                         <  2:1840715/149‑1 (MQ=255)
                ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAAT‑AAATAAatt                                                                                                                                        >  2:840047/1‑149 (MQ=255)
                  accacTTGCATCGTATCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAattat                                                                                                                                      <  2:4027281/149‑1 (MQ=255)
                   ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATAT‑aattaaattaat                                                                                                                                     >  1:3812195/1‑147 (MQ=255)
                   ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAattatt                                                                                                                                     <  2:2184914/149‑1 (MQ=255)
                   ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAattatt                                                                                                                                     <  2:1111951/149‑1 (MQ=255)
                    cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTAttt                                                                                                                                    <  1:52481/149‑1 (MQ=255)
                    cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTAttt                                                                                                                                    <  1:2777416/149‑1 (MQ=255)
                    cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTAttt                                                                                                                                    <  1:2777409/149‑1 (MQ=255)
                    cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTAttt                                                                                                                                    <  2:2496962/149‑1 (MQ=255)
                    cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTAttt                                                                                                                                    <  1:861271/149‑1 (MQ=255)
                      cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATAA‑AAATAAATTAttttt                                                                                                                                  >  2:3729134/1‑147 (MQ=255)
                       ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAAAAT‑AATGAAAATAtttttt                                                                                                                                 >  2:2266464/1‑149 (MQ=255)
                       ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGtt                                                                                                                                 <  2:904053/149‑1 (MQ=255)
                          cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATAT‑AAATAAATTATtttttttt                                                                                                                              >  2:4233110/1‑149 (MQ=255)
                           aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTa                                                                                                                             <  1:3632154/149‑1 (MQ=255)
                           aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTa                                                                                                                             <  1:3631334/149‑1 (MQ=255)
                           aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTa                                                                                                                             <  2:304314/149‑1 (MQ=255)
                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAAT‑AATGAAATTATttttttttta                                                                                                                            >  2:1757782/1‑147 (MQ=255)
                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGAATTAAAAAAAT‑AAATAAAATATTTTTTTTTaa                                                                                                                            >  1:1321112/1‑149 (MQ=255)
                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAAT‑AAATAAATTATTTTTTTTTaa                                                                                                                            >  1:2771344/1‑149 (MQ=255)
                              gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAAT‑AATTAAAATATTTTTTTTTAAat                                                                                                                          >  2:4207351/1‑149 (MQ=255)
                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAata                                                                                                                         <  1:3311312/149‑1 (MQ=255)
                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAata                                                                                                                         <  2:2472401/149‑1 (MQ=255)
                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAata                                                                                                                         <  1:995618/149‑1 (MQ=255)
                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGATTTAAAAAAAA‑AATTAAAATATtttttttttattt                                                                                                                         >  1:1154199/1‑144 (MQ=255)
                                ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAatat                                                                                                                        <  2:4315258/149‑1 (MQ=255)
                                 tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATc                                                                                                                       <  1:2381786/149‑1 (MQ=255)
                                 tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATc                                                                                                                       <  1:4374107/149‑1 (MQ=255)
                                   gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTGATTTAAAAAAAA‑AATGAAATTATTTTTTTTTAAaaatata                                                                                                                     >  1:2129074/1‑146 (MQ=255)
                                    agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCgag                                                                                                                    <  1:2742318/149‑1 (MQ=255)
                                    agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAAAAA‑AAATAAAATATTTTTTTTTAAATATAgaa                                                                                                                    >  1:651819/1‑148 (MQ=255)
                                    agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAAA‑AAATAAATTATTTGTTTTTAAATATagag                                                                                                                    >  2:1340175/1‑149 (MQ=255)
                                    agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTAGTTTTAAAAAAAT‑AATGAAATTATTTTTTTTTAAATATagag                                                                                                                    >  1:651762/1‑149 (MQ=255)
                                      aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                  <  1:3827191/149‑1 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                             <  1:1701450/149‑1 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGTTTTGAAAATAT‑AATTAAATTATTTTTTTTTTAATATAGAGATAACGa                                                                                                             >  1:3126775/1‑149 (MQ=255)
                                            aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                            <  2:3772621/149‑1 (MQ=255)
                                             atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATAT‑AATGAAATTATTTGTTTTTAAATATAGAGATAACGAta                                                                                                           >  2:1070252/1‑148 (MQ=255)
                                             atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATc                                                                                                           >  2:1070345/1‑149 (MQ=255)
                                                   aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTAGATTTCAAAATAA‑AATGTAATTAATTTTTTTTTAATATAGAGCTAACGATCAC‑aaaa                                                                                                    >  2:3387555/1‑149 (MQ=255)
                                                    aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑aaaaa                                                                                                   <  2:4416615/149‑1 (MQ=255)
                                                       ccGTAACCTCATAACCGCCGCGCTGTGCGTTGTTCAGAATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTAAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGa                                                                                                <  1:1624100/149‑1 (MQ=255)
                                                        cgtaaCCGAATAACCGCCGCGGGGTGCGTTTTTCAGAATCTGCTTTGTCGATACCACGTAATTTTTAATATCCTGTCGTTTGTTTTCTTTTTAAAAATAT‑AATTAATTTTTTTTTTTTTAAATATCGAGATAACGATCAC‑AAACACGAc                                                                                               <  1:4195801/146‑1 (MQ=255)
                                                        cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGAc                                                                                               <  2:1303781/149‑1 (MQ=255)
                                                          gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGGGTGTTAGATTTGAAAATAT‑AATGTAATTATTTTTTTTTTAATATGGTGATAACGTTGAC‑AAAAACGACaa                                                                                             >  1:3479040/1‑149 (MQ=255)
                                                          gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGGGGTTTGGGGTTAAAAAAAA‑AATTTAATTATTTTTTTTTTAATTACGTGAGAACCAAAAC‑CAAAAcaacaa                                                                                             >  1:2738855/1‑149 (MQ=255)
                                                                     ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATAT‑AATGAAAATATTTTTTTTTAAATATAGAGATAAAGATAAC‑AAAAACGAAAATATGAAAatta                                                                                  >  2:703309/1‑149 (MQ=255)
                                                                        ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGGGTTTTTGTTTTGAAAAAATAAATAAAATAATTTTTTTTTAAA‑ATATAGATAAATATAAA‑AAAAAAAAAAAAAAGAAAaatatta                                                                               >  1:3923725/1‑148 (MQ=255)
                                                                            gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGAGTTCAAAATAT‑TATTAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑CAAAACGACAAAATGAAAATTATTCgatg                                                                           >  2:4314236/1‑147 (MQ=255)
                                                                               gtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCCAAATAT‑AATGAAATTATTTGTTTTTAAAAATAGAGAAAACCAACAC‑AAAAAAGACAAAATGTAAATTTTTCgcggagg                                                                        >  2:2912940/1‑148 (MQ=255)
                                                                                         ctgcttCTGTTTTGTCTATACCACTTAAATTTTAATATCCTGTGTTTTTTTTTCTATTTAAAAATAT‑ATTTAAATTATTTGTTTTTAAATATCGATATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCaaa                                                             <  1:2509058/144‑1 (MQ=255)
                                                                                            cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTGGTTTTGAAAAAAT‑AAATCAAATATTTTTTTTTTAATATATAGATAAACAAAAA‑AAAAAAGAAAAAAAAAAAAATAATAGAGGAGT‑GAAACGCaaaaaa                                                          >  2:3327670/1‑149 (MQ=255)
                                                                                             atcttttttGTCGATACCAATTAATTTTTCATATCCTGTGTTTTTTTTTCTATTAAAAAATAT‑AATTAAATTTTTTTTTTTTAAATATCGATATAACTAACAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCaaaaaaa                                                         <  1:1135069/143‑1 (MQ=255)
                                                                                             aTCTGTTTTGTCGACACCACTTAATTTTTCATCTCCTGTTTTTTGTTTTATTTTACAAAATAT‑AATTATATTTTTTTTTTTTAAATATCTATTAAACGAACAC‑AAACACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCaaaaaaa                                                         <  1:3361913/149‑1 (MQ=255)
                                                                                              tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATAT‑AATGAAATTATTTTTTTTTAAATATAGAGGTAAAGATCAC‑AAAAAAGACAATATTAAAATTATTAGAGGAGT‑GAAAGGCCTAAAAAc                                                        >  2:3251891/1‑149 (MQ=255)
                                                                                              tCTGCTTTGTCGATACCACGTACATTTTCATAGCCTGTCTTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTTTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAAc                                                        <  2:3556408/149‑1 (MQ=255)
                                                                                                   tttGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTCGATTTGAAAATAT‑AAATTAATTATTTTTTTTTTAATATCGAGATAAAGGTAAA‑CAAAACGAAAAAATTTAAAATATTTGAGGTGG‑GGAAGGCAAAAAAAAGGcca                                                   >  2:1557034/1‑148 (MQ=255)
                                                                                                   tttGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGTATTAAAAATAT‑AAATAAAATATTTTTTTTTATATATAGATATAAACAAAAA‑AAAAAAAAAAAAATTAAAAATAATAGTGGAGT‑GTAATGCaaaaaaaatgcca                                                   >  2:776357/1‑143 (MQ=255)
                                                                                                   tttGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTTAAAATAA‑AATTAAATTTTTTTTTTTTTTATATAGAGATAACGATCACAAAAAAAGAAAAAAT‑AAAAATATTAGAGGAGTGGAAGGGCAAAAAAACgcca                                                    >  2:776183/1‑146 (MQ=255)
                                                                                                    ttGTCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCTATTTCAAAATAT‑AATTAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTc                                                  <  2:1376121/149‑1 (MQ=255)
                                                                                                      gTCTATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATAT‑AATTAAATTATTTTTTTTTAAATATCTAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTccc                                                <  2:651762/149‑1 (MQ=255)
                                                                                                      gTCGATACCACTTAAATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑ATTGAAATTATTTGTTTTTAAATATCGAGATACCGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTccc                                                <  2:651819/149‑1 (MQ=255)
                                                                                                        cGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTGGGTTTGAAAATAT‑AATGTAAATATTTTTTTTTAAATATAGAGATAAAGATCAC‑AAAAAACAAAAAATTAAAATTATTAGGGGAGT‑GAAAGGCCAAAAAACGGCCTACCGa                                              >  2:2689468/1‑149 (MQ=255)
                                                                                                         gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTTTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGAt                                             <  2:3254494/149‑1 (MQ=255)
                                                                                                           tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATAT‑AATGAAATTATTTTTTTTTAAATATCGAGATAACCATAAC‑AAAAACGACAAAATGAAAATTATTCTAGGAGT‑GAAAGGCAAAAAAAAGGCCTCCCGagag                                           >  2:2030462/1‑149 (MQ=255)
                                                                                                           tACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATAT‑AATGAAATTATTTTTTTTTAAAAATAGAGATAAACATCAC‑AAAAAAGACAAAATTAAAATTATTCGAGGCGT‑GTAAGGCAAAAAAACGGCCTCCCCATAg                                           >  2:4021648/1‑149 (MQ=255)
                                                                                                           tACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAAAAT‑AAATAAATTATTTTTTTTTTAATATCGTGCTAAAGATAAC‑AAAAACCACAAAATGAAAATAATTAGAGGCGT‑GTAAGGCAATAAAAAAGCCAcccccgag                                           >  2:1863939/1‑144 (MQ=255)
                                                                                                           tACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAATAT‑AATGAAAATATTTTTTTTTAAATATAGAGATAACGATAAC‑AAAAAAGACAAAATTAAAATTATTCTAGGCGT‑GTAAGGCAAAAAAAAGGCCTCCCGagag                                           >  2:3849375/1‑149 (MQ=255)
                                                                                                             ccACGTACATTTTCATATCCTGTCGTGTTTTTTAGTTTTGAAAATAT‑AATGAAATTATTTTTTTTTTAATATCGAGATTAAGATAAC‑AAAAAAGCCCAAATGAAACTTATTGGAGGGGT‑GGGAGGCACCAAAAAGGCCAcccccggggg                                         >  2:3839303/1‑142 (MQ=255)
                                                                                                               aCGTACATTTTCATATCCTGTCGTGTGTTTTCGGTTTGAAAATAT‑AATGAAATTATTTTTTTTTTAATATAGAGATAAAGATCAC‑AAAAAAGACAAAATTGAAATTATTAGAGGGGT‑GAAAGGCCCAAATAAGGCCACCCCAGAGGGaa                                       >  2:2882500/1‑149 (MQ=255)
                                                                                                               aCGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAAAAT‑AATGTAAATATTTTTTTTTAAAAATAGAGATAAAGATAAC‑AAAAAAGACAAAAATAAAATTATTAGAGGAGT‑GAAAGGCAAAAAAAAGGCCGCCCCAGAGGGaa                                       >  2:680852/1‑149 (MQ=255)
                                                                                                                cgtaatttttCATCTCCTGTGTTTTGTTTTCGATTTCAAAATAT‑AATTAATTTTTTTTTTTTTAATTATCGATATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                      <  1:1788648/143‑1 (MQ=255)
                                                                                                                cGTACATTTTCATAGCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATACCGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                      <  1:1789062/149‑1 (MQ=255)
                                                                                                                 ttaatttttCATATCCTGTCTTTTTTTTTCTTTTTCAAAATAT‑ATTTAATTTTTTTTTTTTTAAATATCGATATAACGATCAA‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                     <  1:1763180/144‑1 (MQ=255)
                                                                                                                 gttctttttAATATCGTTTCGTTTTTTTTAGATTAAAAAATAA‑AATTAAATTATTTTTTTTTAAATATCTTGATAACTATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                     <  2:1639348/144‑1 (MQ=255)
                                                                                                                 gTACATTTTCATATCCTGTCGTTTTTTTTCTATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACTATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                     <  2:723514/149‑1 (MQ=255)
                                                                                                                  tACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCTATATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                    <  2:1673293/149‑1 (MQ=255)
                                                                                                                  tACATGTTCCTCCCCGGTCGTTTGTTTGCGATTTCAAAATCT‑ACTGACATTATTTGTTTTTAACTATCGAGATACCGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                    <  2:1673506/149‑1 (MQ=255)
                                                                                                                    cATTTTCATATCCGGTCGTTTGTTTTCTATTACAAAATAT‑AATGAATTTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGt                                  <  2:3550617/149‑1 (MQ=255)
                                                                                                                             agccTGTCTTTTGTTTTCGATTTCAAAATAT‑AATTAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                         <  2:1258490/147‑1 (MQ=255)
                                                                                                                                     gtttgtttTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                 >  2:313867/1‑149 (MQ=255)
                                                                                                                                      tttgtttTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                >  2:3469958/1‑149 (MQ=255)
                                                                                                                                                aTTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa      >  1:2445489/1‑149 (MQ=255)
                                                                                                                                                   tCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATACCGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc   <  2:2790004/149‑1 (MQ=255)
                                                                                                                                                    cAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg  >  1:4424526/1‑149 (MQ=255)
                                                                                                                                                    cAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg  >  2:75019/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAC‑AAAAACGACAATATGAAAATTATTCGAGGAGT‑GAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCG  >  CP009273/2170565‑2170861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A