Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,713 C→A 25.9% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7130CA25.9% 350.4 / 26.9 192intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (42/83);  new base A (28/22);  total (87/105)
Fisher's exact test for biased strand distribution p-value = 9.99e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

GGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAT  >  CP009273/2170567‑2170858
                                                                                                                                                  |                                                                                                                                                  
ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                   <  1:1472793/148‑1 (MQ=255)
ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                   <  1:382317/148‑1 (MQ=255)
ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                   <  1:2820771/148‑1 (MQ=255)
  cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaa                                                                                                                                                 <  2:880997/148‑1 (MQ=255)
   aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                <  1:3502022/148‑1 (MQ=255)
     cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                              <  2:817593/148‑1 (MQ=255)
       gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                            <  2:628400/148‑1 (MQ=255)
       gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                            <  2:3653070/148‑1 (MQ=255)
        tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAATATaa                                                                                                                                           >  1:1204915/1‑148 (MQ=255)
        tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                                                                           <  2:710928/148‑1 (MQ=255)
        tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTATATTTCAAAATATaa                                                                                                                                           >  2:328463/1‑148 (MQ=255)
           cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTATCGTTTGTTTTCGATTTCAAAATATAATGa                                                                                                                                        <  1:901695/148‑1 (MQ=255)
            tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGAGACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                       <  2:2014895/148‑1 (MQ=255)
             accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                      <  1:1740322/148‑1 (MQ=255)
              ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTGTTTTAAAAATATAATTAAat                                                                                                                                     >  2:1002909/1‑148 (MQ=255)
               caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                    <  1:3170390/148‑1 (MQ=255)
                accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatta                                                                                                                                   <  1:1568563/148‑1 (MQ=255)
                 ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                  <  2:42110/148‑1 (MQ=255)
                   acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTGAAAATATAATGAAATTAttt                                                                                                                                >  2:2861805/1‑148 (MQ=255)
                   acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                <  2:2722200/148‑1 (MQ=255)
                    cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATTAAAATAtttt                                                                                                                               >  2:1021888/1‑147 (MQ=255)
                    cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATTAAAATATTTg                                                                                                                               >  2:3599867/1‑148 (MQ=255)
                     ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                              <  2:2618872/148‑1 (MQ=255)
                     ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                              <  1:403288/148‑1 (MQ=255)
                     ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTAAAATATTTGt                                                                                                                              >  2:3757255/1‑148 (MQ=255)
                      tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                             <  1:1809499/148‑1 (MQ=255)
                      tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGTTTTTAAAAAATAATTTAAATAtttttt                                                                                                                             >  1:459686/1‑148 (MQ=255)
                       gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttt                                                                                                                            <  1:533611/148‑1 (MQ=255)
                        cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtttt                                                                                                                           <  1:3054965/148‑1 (MQ=255)
                         aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAAAAAAAATAAAATAAtttttttt                                                                                                                          >  1:952218/1‑148 (MQ=255)
                          tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAAATAAATTATttttttttt                                                                                                                         >  2:2743116/1‑147 (MQ=255)
                          tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTATATTTAAAAAAATAAATAAATTATttttttttt                                                                                                                         >  1:886953/1‑147 (MQ=255)
                          tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCATTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTTTTTTT‑a                                                                                                                        >  2:3628524/1‑148 (MQ=255)
                           cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑aa                                                                                                                       <  2:1867511/148‑1 (MQ=255)
                           cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑aa                                                                                                                       <  1:2659265/148‑1 (MQ=255)
                           cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑aa                                                                                                                       <  1:2582981/148‑1 (MQ=255)
                           cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGGGTTTTTTATTTTAAAAAAAAAAAAAAATATttttttttta                                                                                                                        >  1:329233/1‑146 (MQ=255)
                            gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑aaa                                                                                                                      <  1:381888/148‑1 (MQ=255)
                             tttCGAGATTGTGAATATTGCATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAat                                                                                                                     <  2:3131282/148‑1 (MQ=255)
                              ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAATTTTTTTTTTT‑AAata                                                                                                                    >  2:2978643/1‑148 (MQ=255)
                                 gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCg                                                                                                                 <  2:1153957/148‑1 (MQ=255)
                                   gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCgag                                                                                                               <  2:3582654/148‑1 (MQ=255)
                                   gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCgag                                                                                                               <  2:1134604/148‑1 (MQ=255)
                                   gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAAAATagag                                                                                                               >  2:2737503/1‑148 (MQ=255)
                                   gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAATATTTGTTTTT‑AAATATctat                                                                                                               >  2:2135214/1‑145 (MQ=255)
                                   gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAATATAATGAAATTATTTTTTTTT‑AAATATCgag                                                                                                               >  2:207761/1‑148 (MQ=255)
                                    aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCgaga                                                                                                              <  1:3375811/148‑1 (MQ=255)
                                    aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCgaga                                                                                                              <  1:1083445/148‑1 (MQ=255)
                                      tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGTTTTCAAAATATAATTTAATTATTTTTTTTT‑TTatatattgttt                                                                                                            >  1:3661593/1‑141 (MQ=255)
                                      tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATa                                                                                                            <  2:1353500/148‑1 (MQ=255)
                                      tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAAAAAAGAAAATATTTTTTTTT‑AAATATCGAGATa                                                                                                            >  2:1404085/1‑148 (MQ=255)
                                      tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAAAATAATGTAATTATTTTTTTTT‑AAATATAGAGTTa                                                                                                            >  1:952534/1‑148 (MQ=255)
                                       gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATaa                                                                                                           <  2:3378407/148‑1 (MQ=255)
                                         gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACg                                                                                                         <  2:224602/148‑1 (MQ=255)
                                            tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTGAAAATATAATGTAAATATTTTTTTTT‑TAATATAGAGTTAAAGATc                                                                                                      >  1:2798861/1‑148 (MQ=255)
                                                 aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGTAATTATTTTTTTTT‑TAATATCGAGATAAAGATAACaaa                                                                                                 >  2:517480/1‑148 (MQ=255)
                                                  aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACaaaa                                                                                                <  1:1002909/148‑1 (MQ=255)
                                                    gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTGAAAAAATAATAAAATTATTTTTTTTT‑TAATATTGAGGTTAAGATaaaaaaaaa                                                                                              >  1:3648489/1‑147 (MQ=255)
                                                         aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTT‑AAGAATAGAGATAAAGATAACAAAAAACACaa                                                                                         >  2:1919802/1‑148 (MQ=255)
                                                         aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAGTTTAGAGATAACGATCACAAAAACGACaa                                                                                         >  2:1919841/1‑148 (MQ=255)
                                                              cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGTTTTAAAAAAATAATTAAAATATTTTTTTTT‑TAATATGGTGATAAAGATCAAAAAAAAGACAatatta                                                                                    >  1:1579562/1‑146 (MQ=255)
                                                                 aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTGAAAATATAATGAAATTATTTGTTTTT‑TAATATCGGGATAACGATCACACAAACGACACTATGgaaa                                                                                 >  2:2786636/1‑148 (MQ=255)
                                                                 aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGATTTGTCGATACCACGTAAATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGATATAACGATCACAAAAACGACAATATGaaaa                                                                                 <  2:2377077/148‑1 (MQ=255)
                                                                  aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTGGTTTTGAAAATATAATTAAAATATTTGTTTTT‑TAATATAGATATAAAGATCACAAAAAAGAAAATATTTAAat                                                                                >  2:1077166/1‑148 (MQ=255)
                                                                       cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGTTTTAAAAAAAAAAATAAAAAATTTTTTTTT‑TTATATATATAAAAAAATAACAAAAAAAACAAAAATAAAattttta                                                                           >  1:2712328/1‑147 (MQ=255)
                                                                          gcTGTGCGTTTTTCACCATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTA‑AAATATCGATATAACGAACAAAAAAACGACAATATGAAAATTATTCgag                                                                        <  2:1658215/148‑1 (MQ=255)
                                                                           cTGTGCGTTGTACAGAATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACCATCACAAACACGACAATATGAAAATTATTCgagg                                                                       <  2:647494/148‑1 (MQ=255)
                                                                              tgCGTTGTACAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGt                                                                    <  2:1537703/148‑1 (MQ=255)
                                                                               gatctgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTTTTTTCTATTTAAAAATATAATTAAATTATTTTTTTTT‑AAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTg                                                                   <  2:23528/144‑1 (MQ=255)
                                                                               gCGTTGTTCACCATCTGCTTTGTCGATACCACGTACATTTTAATAACCTGTGTTTTGTTTTCTATTTAAAAATATAATTAATTTTTTTTTTTTA‑AAATATCGAGAAAACGAACACAAACACGACAATATGAAAATTATTCGAGGAGTg                                                                   <  1:522053/148‑1 (MQ=255)
                                                                                 gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTGATTTAAAAATTTTATTAAAATATTTTTTTTT‑TAATATCGAGATAAAGATCAAAAAAACGACAAAATGAAAATTATTATGGGAGTgta                                                                 >  2:3609522/1‑146 (MQ=255)
                                                                                 gtgtttCAGAATCTGCTTTGTCGATACAACTTACATTTTAATATCCTGTCTTTTTTTTTCGTTTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaa                                                                 <  2:2592512/144‑1 (MQ=255)
                                                                                   tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTTAAAATATAAATAAAATATTTTTTTTT‑TAAAATAGAGATAAAGATAACAAAAAACAAAAAATTAAAATTTTTTGAGGGGTTaaaa                                                               >  1:2195763/1‑147 (MQ=255)
                                                                                   tgttCAGCATCTGATTTGTCGATCCCCCGTAATTTTTAATATCCTGTCTTTTGTTTTCGATTTAAAAATATAATGAATTTTTTTTTTTTT‑AAATATCGAGATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAg                                                               <  1:489561/148‑1 (MQ=255)
                                                                                            tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTAAAAATATAATTTAAATATTTTTTTTT‑TAATATAGTCATAAAGAAGAAAAAAAAGACAAAATTTAAAATATTAGTTGAGTTGAATGCaaaaaaa                                                      >  2:366043/1‑148 (MQ=255)
                                                                                             cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAAAGAAAATAATTTTTTTT‑AAATATAGAGATAAACATAAAAAAAAACACAAAAATAAAAAAAATAGAGGAGTTGAATGCaaaaaaaa                                                     >  1:635977/1‑147 (MQ=255)
                                                                                             cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATTTAATTATTTGTTTTT‑AAAAATCGAGATAACCATCACAAAAAAGAAAAAATGAAAATTATTAGCGGAGTGAAAGGCaaaaaaaa                                                     >  2:938841/1‑147 (MQ=255)
                                                                                                  ttGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAAAAAAAATAAATAATTTTTTTTT‑AAAAATCGAGATAAACATAAAAAAAAAAACAAAAATAAAAATATTATTGGGGTGTAAGGGaaaaaaaatccca                                                >  2:2885958/1‑142 (MQ=255)
                                                                                                      cGATACCACTTAATTTTTAATATCCTTGGTTTTTTTTTCTATTAAAAAATATATTTAATTTTTTTTTTTTT‑AAATATCTTTAAAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCg                                            <  1:2743116/148‑1 (MQ=255)
                                                                                                          aCCACGTAAATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                        <  2:84589/148‑1 (MQ=255)
                                                                                                            cACGTAAATTTTCATATCCTGTCTTTTGTTTTCTATTTCAAATTAAAATTAATTTTTTTTTTTTA‑AAATATCGATATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                      <  1:2476639/148‑1 (MQ=255)
                                                                                                              cGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAATATAATTAAAATATTTGTTTTT‑TAATATAGAGTTAACGATCACAAAAAACACAAAATTAAAAATATTTTAGGGGTGTAATGGAAAAAAAACGCCTCCCCATAggggc                                    >  2:2953750/1‑146 (MQ=255)
                                                                                                               ttACTTTTTAATTTCCTTTGTTTTGTTTTGGTTTTAAAATTATATTTAAATT‑TTTGTTTTTAAAATATCGATATAACTATAAAAAAAACGACAATATGAAAATTATTCGTGGATTGAATGGCAAAAAAACGGCCTCCCGATAGGGAAg                                   <  2:324541/147‑1 (MQ=255)
                                                                                                                tACATTTTCAGATCCTGTCGTTTTTTTTCGATTTAAAAATATAATTAAATTATTTGTTTTT‑AAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                  <  2:1329768/148‑1 (MQ=255)
                                                                                                                 aCATTTTCATATCCTGTCGTGTGTTTTAGCTTTAAAAATATAATGAAAATATTTTTTTTT‑AAATATAGAGATAAAGATCACAAAAACGACAATATGGAAAGTATTAGCGGAGGGAAAGGCAAAAAAAAGGCCTCCCGCGAGGGGAGcc                                 >  2:2397784/1‑148 (MQ=255)
                                                                                                                       tCATCTCCGGTCTTTTGTTTTCTATTTAAAAATATAATTAAATTATTTGTTTTT‑AAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCa                           <  2:3661593/148‑1 (MQ=255)
                                                                                                                                 tCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACTATCACAAAAAAGACAATAAGAAAATTATTCGAGGACTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                 >  2:182477/1‑148 (MQ=255)
                                                                                                                                  cttttgtttTATATTAAAAAATATAATAAAATTTTTTTTTTTA‑AAATATCCAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                <  2:969710/146‑1 (MQ=255)
                                                                                                                                  cGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                <  2:175238/148‑1 (MQ=255)
                                                                                                                                    tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt              >  1:2316305/1‑148 (MQ=255)
                                                                                                                                    tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt              >  1:3783880/1‑148 (MQ=255)
                                                                                                                                    tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt              >  1:3578013/1‑148 (MQ=255)
                                                                                                                                    tttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt              >  1:1166691/1‑148 (MQ=255)
                                                                                                                                     tttttttCTATTTCAAAATATAATCAAATTATTTTTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt             <  2:716906/145‑1 (MQ=255)
                                                                                                                                     tttttttCGATTAAAAAATATAATTAATTTATTTTTTTTA‑AAATATCGAGATAACTAAAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt             <  1:34873/145‑1 (MQ=255)
                                                                                                                                         tttCTATTAAAAAATATATTTAATTTTTTTTTTTTA‑AAATATCTAAAAAACTACCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt         <  1:2952774/148‑1 (MQ=255)
                                                                                                                                         tttCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt         >  2:1994526/1‑148 (MQ=255)
                                                                                                                                                ttCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt  >  1:45515/1‑148 (MQ=255)
                                                                                                                                                ttCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt  >  1:455547/1‑148 (MQ=255)
                                                                                                                                                ttCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt  >  1:2127338/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTT‑AAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAT  >  CP009273/2170567‑2170858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A