Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,713 C→A 20.1% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7130CA20.1% 570.3 / 28.8 267intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (48/141);  new base A (45/18);  total (108/159)
Fisher's exact test for biased strand distribution p-value = 1.33e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 273 total aligned reads displayed.

GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  CP009273/2170564‑2170862
                                                                                                                                                     |                                                                                                                                                       
   ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCaaa                                                                                                                                                      <  2:2327193/150‑1 (MQ=255)
    gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                     <  2:2869577/150‑1 (MQ=255)
       gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                                  <  2:3916339/150‑1 (MQ=255)
       gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                                  <  2:3830017/150‑1 (MQ=255)
         ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTATATTTGAAAAAATaa                                                                                                                                                >  1:898824/1‑150 (MQ=255)
         ggTTTCTACCACCACGTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                                                                                <  2:428219/150‑1 (MQ=255)
           tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑Tg                                                                                                                                            <  1:1554646/150‑1 (MQ=255)
              cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGaaa                                                                                                                                         <  2:529633/150‑1 (MQ=255)
               tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAat                                                                                                                                        <  2:317358/150‑1 (MQ=255)
                accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAatt                                                                                                                                       <  1:3153065/150‑1 (MQ=255)
                accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAatt                                                                                                                                       <  2:2401983/150‑1 (MQ=255)
                 ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAatta                                                                                                                                      <  1:1088708/150‑1 (MQ=255)
                 ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATATAA‑‑TTAAaata                                                                                                                                      >  2:2712032/1‑150 (MQ=255)
                  caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAattat                                                                                                                                     <  1:1656671/150‑1 (MQ=255)
                   accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAattatt                                                                                                                                    <  2:4189615/150‑1 (MQ=255)
                   accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAattatt                                                                                                                                    <  2:1105668/150‑1 (MQ=255)
                   accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAattatt                                                                                                                                    <  1:600452/150‑1 (MQ=255)
                    ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTAttt                                                                                                                                   <  2:4736784/150‑1 (MQ=255)
                    ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTAttt                                                                                                                                   <  2:2119644/150‑1 (MQ=255)
                     cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTg                                                                                                                                  <  1:2562379/150‑1 (MQ=255)
                      acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGt                                                                                                                                 <  1:5119907/150‑1 (MQ=255)
                      acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGt                                                                                                                                 <  1:410812/150‑1 (MQ=255)
                       cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAA‑‑TTAAATTAtttttt                                                                                                                                >  1:3558978/1‑150 (MQ=255)
                        ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGttt                                                                                                                               <  2:796802/150‑1 (MQ=255)
                        ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGttt                                                                                                                               <  1:1166069/150‑1 (MQ=255)
                          gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGttttt                                                                                                                             <  1:3783970/150‑1 (MQ=255)
                          gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGttttt                                                                                                                             <  2:1172929/150‑1 (MQ=255)
                           cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAAAATAA‑‑TTAAATTATTTGTTTTTa                                                                                                                            >  2:2546418/1‑150 (MQ=255)
                           cATCGTTTCGAAATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTa                                                                                                                            <  1:4218025/150‑1 (MQ=255)
                             tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTaaa                                                                                                                          <  1:2352426/150‑1 (MQ=255)
                              cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGATTTAAAAAAAAAA‑‑TTAAAATTTTTTTTTTTTAat                                                                                                                         >  1:16084/1‑150 (MQ=255)
                               gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGGTTTAAAAAAAAAAATTAAAAATATTTTTTTTTaaa                                                                                                                          >  1:1389083/1‑150 (MQ=255)
                               gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAata                                                                                                                        <  1:282279/150‑1 (MQ=255)
                                tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAatat                                                                                                                       <  1:3888591/150‑1 (MQ=255)
                                tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAatat                                                                                                                       <  1:4116956/150‑1 (MQ=255)
                                 ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATc                                                                                                                      <  2:4382555/150‑1 (MQ=255)
                                 ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATc                                                                                                                      <  1:4770536/150‑1 (MQ=255)
                                 ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGAGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATc                                                                                                                      <  2:4888140/150‑1 (MQ=255)
                                 ttCGAGATTGTGAATATGGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATc                                                                                                                      <  2:2513780/150‑1 (MQ=255)
                                  tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCg                                                                                                                     <  2:2128430/150‑1 (MQ=255)
                                   cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCga                                                                                                                    <  1:733262/150‑1 (MQ=255)
                                   cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCga                                                                                                                    <  1:732988/150‑1 (MQ=255)
                                    gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTTAAAAAATAA‑‑ATAAAATATTTTTTTTTTAATATAgat                                                                                                                   >  1:3659563/1‑149 (MQ=255)
                                    gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAATATAA‑‑TGTAATTATTTGTTTTTAAatttaggg                                                                                                                   >  1:4433831/1‑144 (MQ=255)
                                     agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATATAA‑‑ATAAAATATTTTTTTTTTAATATAgata                                                                                                                  >  1:2907432/1‑148 (MQ=255)
                                      gagtgtgAATAGTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                 <  1:4467831/147‑1 (MQ=255)
                                       aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTTTGGTTTCGAATTCAAAATATAA‑‑AGAAATAATTTTTTTTTAAATATAGAtata                                                                                                                >  2:3024741/1‑150 (MQ=255)
                                              atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                         <  1:3496473/150‑1 (MQ=255)
                                               tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATATAA‑‑TTAAATTATTTTTTTTTTTATATATATATAAAGATcaa                                                                                                        >  1:1999593/1‑149 (MQ=255)
                                                atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                       <  2:1294926/150‑1 (MQ=255)
                                                atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                       <  2:4518662/150‑1 (MQ=255)
                                                    aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                   <  2:1094420/150‑1 (MQ=255)
                                                     aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                  <  1:3118432/150‑1 (MQ=255)
                                                        ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTTGGATTTGTAAATATAA‑‑TTTAATTATTTGTTTTTTTATATAGAGATAAAGATAAAAAAAAAGAc                                                                                               >  2:1829835/1‑150 (MQ=255)
                                                         cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAA‑‑TTAAATTATTTTTTTTTTAATATAGAGATTAAGATAACAAAAACGACa                                                                                              >  1:5225769/1‑150 (MQ=255)
                                                                gcttAACCGCCGCGCTTTGCGGTGTTCAGCATCTGCTTTGTCGATACCACTTACTTTTTCATATCCTGTCGTTTGTTTTCTTTTTCAAAATATAT‑‑TGAAATTATTTGTTTTTAAATATCGTGATAACGATCAAAAAAACGACAATATGaa                                                                                       <  2:4431494/147‑1 (MQ=255)
                                                                gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTGAAAAAATAA‑‑TTTAATTATTTTTTTTTTAATATAGAGATAACGCTCACAAAAACGACAAAATgta                                                                                       >  1:3985513/1‑148 (MQ=255)
                                                                 cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTGAAAAAAAAA‑‑TTAAAATATTTTTTTTTTAATATGGATATAAAAATAAAAAAAAAAACAAAAAGaaa                                                                                      >  1:459751/1‑150 (MQ=255)
                                                                    aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACTTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatt                                                                                   <  2:2825086/150‑1 (MQ=255)
                                                                      ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATCCCCCTTACATTTTCATATCCTGTCTTTTTTTTTCGATTTAAAAATATAA‑‑TTAATTTATTTTTTTTTAAATATCTAGATAACGAACACAAACACGACAATATGAAAattat                                                                                 <  1:2578222/150‑1 (MQ=255)
                                                                        gccgcGCTGTGCGTTGTACAGCATCTGCTTTTTCGATACCACGTACATTTACATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                               <  2:317233/150‑1 (MQ=255)
                                                                         ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCg                                                                              <  2:3694869/150‑1 (MQ=255)
                                                                              cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAA‑‑TGAAATTATTTTTTTTTAAATATAGAGATAAAGATAACAAAAAAGACAAAATTTAAATAATTGgaggtg                                                                         >  2:3124542/1‑148 (MQ=255)
                                                                              cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTAAAAAAATAA‑‑TTTAAATATTTTTTTTTTAATATAGAGATAAAGATAAAAAAAAAGAAAAAATTAAAaatattagtggtg                                                                         >  1:5169399/1‑143 (MQ=255)
                                                                                gtgGGTTGTTCAGCATCTGCTTTGTCGATACCAGGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAACACGACAATATGAAAATTATTCGAGGAGTg                                                                       <  2:2083597/150‑1 (MQ=255)
                                                                                 tgCGTTGTCCCGCATCTGCTTTGTCGATACCCCTTACTTTTTAATATCCTGTCTTTTGTTTTCTATTACAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGa                                                                      <  1:4354656/150‑1 (MQ=255)
                                                                                  gCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTTTTTTTTAATATCGAGATAACGATCACAAAAAAGACAAAATTCAAATTATTCGGGGGGTgtt                                                                     >  2:3294142/1‑148 (MQ=255)
                                                                                             cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGAATTAAAAATATAA‑‑TTAAATTATTTTTTTTTTAATATAGAGTTAACGATAACAAAAAAGAAAATATTAAAATTTTTAGAGGTGTGTAATGCaaaaaaa                                                          >  1:2674339/1‑150 (MQ=255)
                                                                                              aTCTGCTTTGCCTATACCACGTACATTTTAATATCCTGTCTTTTTTTTTCTATTTCAAAATATAA‑‑TTAATTTATTTTTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  1:228523/150‑1 (MQ=255)
                                                                                                cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAAAAAACAAAAAAAAA‑‑TGAAAATATTAGTTTTTAAAAATCAAAATAAAAGTCACAAAAAAGAAAATAAGAAAAAAATTAAAAGAATGAAATGaaaaaaaaacg                                                       >  2:2451845/1‑147 (MQ=255)
                                                                                                  gatttGTCGATACAACTTAAATTTTCATATATTGTGGTTTTTTTTCTTTTTAAAAATTTAA‑‑TGAATTTTTTTTTTTTTAAATTGCTATATAACGATCACAAAAACGAAAATATGAAAATTATTCGAGGAGTTAAAGGCAAAAAAACGGcc                                                     <  2:3993747/148‑1 (MQ=255)
                                                                                                    tttGTCTATACCACTTAAATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAA‑‑TTAAATTATTTGTTTTTAATTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTc                                                   <  2:147849/150‑1 (MQ=255)
                                                                                                       gTCGATACCACGTAAATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TTAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCg                                                <  2:4677139/150‑1 (MQ=255)
                                                                                                            tACCACGTAATTTTTCATATCCTGTCTTTTTTTTTCGTTTTAAAAATATAA‑‑TTAAATTTTTTGTTTTTAATTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAgg                                           <  1:1799071/150‑1 (MQ=255)
                                                                                                                 cGTACATTTTCATATCCTTTCGTTTGTTTTCGATTAAAAAATATAA‑‑TTAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                      <  2:5135169/150‑1 (MQ=255)
                                                                                                                  ttaatttttCATATCCTTTTTTTTGTTTTCTTTTTAAAAATATAT‑‑TTAAATTATTTGTTTTTAAATTTCTATATAACTATAAAAAAAACGACTATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGcc                                     <  2:898824/145‑1 (MQ=255)
                                                                                                                   tACATTTTCATATCCTGTCGTGTGTTTTTGTTTTGAAAATATAA‑‑TGTAAATATTTTTTTTTTAATTTGTAGTGAACGATAACAAAAAAGACCAAAAGAAAATTTTTTGTGGGGGGTTTGGCAAAATAAAGGCCGCCCGAGAGGGGAGccc                                    >  2:4964271/1‑149 (MQ=255)
                                                                                                                     atttttCATATCGTGTTTTTTTTTTTCTTTTAAAAAATATAA‑‑TTAATTTTTTTGTTTTTAAATATCTACAAACCGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                  <  1:2409162/148‑1 (MQ=255)
                                                                                                                             tatCCTGTCGTTTGTTTTATAATTAAAAAAAAAA‑‑ATAAAATATTTTTTTTTTAATATAGATATAAAGATAAAAAAAAAAAAAAAAATAAAATTAATAGAGGAGTGAAAGGCAAAAAAACGGCCACCCGCTAGGGAAACCCAAGCacaatg                          >  2:3278839/1‑145 (MQ=255)
                                                                                                                                ccTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGTAGCCGTAGCAAAGTgcgc                       >  2:3566988/1‑150 (MQ=255)
                                                                                                                                 cTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcg                      >  2:1659583/1‑150 (MQ=255)
                                                                                                                                 cTGTCGTTTGTTTTCGATTTAAAAATATAA‑‑TTAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGACGTATCAAAGTGcgcg                      >  1:805045/1‑150 (MQ=255)
                                                                                                                                  tGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     >  2:3674915/1‑150 (MQ=255)
                                                                                                                                   gTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCCAAGTGCGCgtg                    >  1:1192411/1‑150 (MQ=255)
                                                                                                                                    tcttttgtttTCTATTTCAAAATATAA‑‑TGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                   <  1:309442/147‑1 (MQ=255)
                                                                                                                                    tCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                   <  2:3985513/150‑1 (MQ=255)
                                                                                                                                      gtttgtttTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                 >  1:1473818/1‑150 (MQ=255)
                                                                                                                                      gtttgtttTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                 >  2:723620/1‑150 (MQ=255)
                                                                                                                                         tgtttTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa              >  1:3307865/1‑150 (MQ=255)
                                                                                                                                          tttttCTATTTAAAAATATAA‑‑TTAAATTATTTTTTTTTAAATTTCTTTATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  1:325271/149‑1 (MQ=255)
                                                                                                                                          gtttTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             >  2:3395845/1‑150 (MQ=255)
                                                                                                                                          gtttTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             >  1:1245924/1‑150 (MQ=255)
                                                                                                                                           ttttCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            >  2:3644790/1‑150 (MQ=255)
                                                                                                                                           ttttCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            <  2:2659987/150‑1 (MQ=255)
                                                                                                                                                gATTTCAAAACATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa       >  2:1527949/1‑150 (MQ=255)
                                                                                                                                                 aTTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt      >  2:4588695/1‑150 (MQ=255)
                                                                                                                                                 aTTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCGCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt      <  2:575826/150‑1 (MQ=255)
                                                                                                                                                    tCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg   >  2:4045090/1‑150 (MQ=255)
                                                                                                                                                     cAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  1:2729835/1‑150 (MQ=255)
                                                                                                                                                     cAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTGTTTATGCCGGATGCgg  >  1:4686599/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
GTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAA‑‑TGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  CP009273/2170564‑2170862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A