Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F10 I0 R1 85 228.3 8665814 85.8% 7435268 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,713 C→A 28.9% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7130CA28.9% 287.1 / 27.5 164intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (34/69);  new base A (30/16);  total (79/85)
Fisher's exact test for biased strand distribution p-value = 3.21e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCG  >  CP009273/2170565‑2170861
                                                                                                                                                     |                                                                                                                                                    
  ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATT‑TGaa                                                                                                                                                    >  2:4016595/1‑149 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCaaaa                                                                                                                                                  <  1:921284/149‑1 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCaaaa                                                                                                                                                  <  2:2016812/149‑1 (MQ=255)
     aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTGTTTGATTGTCaaat                                                                                                                                                  >  1:2540563/1‑148 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAata                                                                                                                                                <  1:2253730/149‑1 (MQ=255)
      gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTGTTTTAGATT‑TAaaaaaa                                                                                                                                                >  2:1127027/1‑147 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAatat                                                                                                                                               <  1:1276922/149‑1 (MQ=255)
        ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAAtata                                                                                                                                              <  2:1970254/149‑1 (MQ=255)
         gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATaa                                                                                                                                             <  2:2914655/149‑1 (MQ=255)
           ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATg                                                                                                                                           <  2:2587994/149‑1 (MQ=255)
           ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATg                                                                                                                                           <  2:4261948/149‑1 (MQ=255)
            gcgaccaccCCGTGCATCGCGTCGCGCTTGTGACGAGTGCCGGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTGTGTCGATACCACGTCCATTTTCAGATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGa                                                                                                                                          <  2:2186478/146‑1 (MQ=255)
             cTACCACCACTTGCATCGTTTCGAGAGTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGaa                                                                                                                                         >  2:2762961/1‑149 (MQ=255)
              tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGaaa                                                                                                                                        <  2:4201960/149‑1 (MQ=255)
               accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAat                                                                                                                                       >  2:2772474/1‑149 (MQ=255)
                 caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATT‑TCAAAATATAATGAAaata                                                                                                                                     >  2:3598388/1‑149 (MQ=255)
                 caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATT‑TAAAAAAAAaattaaatta                                                                                                                                     >  1:3215125/1‑149 (MQ=255)
                   ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TAAAAATAAAAAGAAattaat                                                                                                                                   >  2:342245/1‑147 (MQ=255)
                   ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATT‑TCAAAATATAAATAAattatt                                                                                                                                   >  2:2747747/1‑149 (MQ=255)
                     acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTGTTT‑TATAAAAAAAAATAAAATAtttt                                                                                                                                 >  1:2938330/1‑148 (MQ=255)
                       ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGtt                                                                                                                               <  2:3114046/149‑1 (MQ=255)
                       ttGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGtt                                                                                                                               <  2:3113985/149‑1 (MQ=255)
                            tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTTTTT‑TTTAAATAAAAAAAAAAAATTTTTTTTTat                                                                                                                          >  1:1088309/1‑148 (MQ=255)
                             cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTaaa                                                                                                                         <  2:2464385/149‑1 (MQ=255)
                             cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTaaa                                                                                                                         <  1:1106431/149‑1 (MQ=255)
                              gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATT‑TAAAAAAAAAATTAAAAAATTTTTTTTTAAat                                                                                                                        >  1:722282/1‑149 (MQ=255)
                              gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGTTT‑TTAAAATATAATTAAATTATtttttttttttt                                                                                                                        >  2:3269935/1‑145 (MQ=255)
                                ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                      <  1:1525681/149‑1 (MQ=255)
                                 tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATc                                                                                                                     <  2:4274168/149‑1 (MQ=255)
                                   gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                   <  2:1884648/149‑1 (MQ=255)
                                    agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATT‑TAAAAATATAAATAAATTATTTTTTTTTAAATATagag                                                                                                                  >  2:1931632/1‑149 (MQ=255)
                                     gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATT‑TAAAAAAATAATTAAAATAATTTTTTTTAAATATAgaga                                                                                                                 >  1:2499666/1‑149 (MQ=255)
                                      aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGAt                                                                                                                <  2:2487840/149‑1 (MQ=255)
                                      aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTGGTTTTTGATT‑TAAAAAAATAATTAAAATATTTTTTTTTTAAAATagagtt                                                                                                                >  1:3337635/1‑147 (MQ=255)
                                      aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGGTTTTTTGATT‑TTAAAATATAATTTAAATATTTTTTTTTTAataaaattat                                                                                                                >  1:3339543/1‑142 (MQ=255)
                                       ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATa                                                                                                               <  1:445387/149‑1 (MQ=255)
                                        tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATaa                                                                                                              <  2:2917125/149‑1 (MQ=255)
                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                             <  1:2219365/149‑1 (MQ=255)
                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                             <  1:2219526/149‑1 (MQ=255)
                                          tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                            <  2:1064511/149‑1 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGTATT‑TAAAAAAATAATGAAATTATTTTTTTTTTAATATAGAGATAAcca                                                                                                           >  2:810570/1‑147 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGa                                                                                                           >  2:2447601/1‑149 (MQ=255)
                                             atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTTATT‑TGAAAATATAAAGTAAATAATTTTTTTTTAATATATTGATAAAGAta                                                                                                         >  1:3450486/1‑148 (MQ=255)
                                              tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                        <  1:11132/149‑1 (MQ=255)
                                              tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                        <  2:1172629/149‑1 (MQ=255)
                                              tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                        <  2:1172477/149‑1 (MQ=255)
                                               atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                       <  2:631525/149‑1 (MQ=255)
                                               atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                       <  2:3617494/149‑1 (MQ=255)
                                               atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                       <  1:1759839/149‑1 (MQ=255)
                                                     tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATT‑TCAAAAAATAAAGAAAATATTTTTTTTTAAATATAGAGATAACGATCACaaaaaa                                                                                                 >  2:3387954/1‑148 (MQ=255)
                                                      gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACAGTTTCATATCCTGTCGTGTTTTTTGGATT‑TGAAAAAATAAATTAATTATTTTTTTTTTAATATAGTGAGTAAGATCACaaacaag                                                                                                >  1:940971/1‑145 (MQ=255)
                                                         ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATT‑TAAAAATATAATGAAATTATTTTTTTTTTAATATAGAGATTACGCTCACaaaaaaaaca                                                                                             >  2:86438/1‑144 (MQ=255)
                                                         ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGTTT‑TCAAAAAAAAATTAAATTATTTTTTTTTTAATATAGAGTTTAAGATCACaaaaaaaaaa                                                                                             >  1:334495/1‑144 (MQ=255)
                                                         ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATT‑TCAAAAAAAAATTTAAATATTTTTTTTTTAATATAGATGTAAAGATAACaaaaaacaaa                                                                                             >  1:4311868/1‑144 (MQ=255)
                                                             ccGAATAACCGCCGCGGTTTGCGTTTTTCAGCATAGGCTTTGTCTATACCACGTACATTTTCATATCCTTTCTTTTGTTTTCTTTT‑TAAAAATATAATTAAATTATTTTTTTTTAAATATCTATATAACTATCACAAACACGACAatat                                                                                         <  2:3839012/149‑1 (MQ=255)
                                                                 aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATT‑TGAAAATATAATTTAATTATTTTTTTTTTAATATAGAGATAACAATAAAAAAAACAACAATATTaaa                                                                                     >  2:964104/1‑149 (MQ=255)
                                                                   aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATT‑TAAAAAAATAATGAAATTATTTTTTTTTAAATATAGATATAAAGAAAAACAAAAAGACAATATGTAAat                                                                                   >  2:584932/1‑149 (MQ=255)
                                                                   aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATT‑TCAAAATATAAATAAATTATTTTTTTTTTAATATATTGATAAAGATCAAAAAAAATACAATATGAAAat                                                                                   >  1:4220178/1‑149 (MQ=255)
                                                                     ccgccgCGCTGTGCGTTGTTCAGCATCTGATTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAatta                                                                                 <  2:2371755/149‑1 (MQ=255)
                                                                      cgccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATT‑TTAAAAAAAAAATTAAAAATTTTTTTTTTTATTTATAGAAAAAGATCACAAAAAACACAAAAAGAAAaataa                                                                                >  1:3703416/1‑148 (MQ=255)
                                                                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATT‑TAAAAAAATAAATTAAATATTTTTTTTTAAATATAGAGATAAAGAAAACAAAAAAGAAAATATTAAAaatatt                                                                               >  2:455959/1‑149 (MQ=255)
                                                                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATT‑TCAAAATATAATTAAATTTTTTTTTTTTTAATATAGAGATAACGAACAAAAAAACGAAAAAATGAAAaataat                                                                               >  2:969051/1‑147 (MQ=255)
                                                                             cTGTGCGTTTTTCAGCATCTGCTTTGTCGATACCACGTACATTTTAATATCCTGTCTTTTGTTTTCGATT‑TCAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCgagga                                                                         <  2:1966972/149‑1 (MQ=255)
                                                                              tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATT‑TAAAAATATAATGTAAATATTTTTTTTTAAATTTCGATATAAAGATCACAAAAAAGACAATATGTAAATTTTTCgaggtg                                                                        >  2:4302721/1‑147 (MQ=255)
                                                                              tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATT‑TAAAAAAATAAATAAAATAATTTTTTTTAAATATAGAGATAAAGAAAACAAAAAACACAAAATTAAAAATAATAgatgag                                                                        >  2:4153619/1‑149 (MQ=255)
                                                                                          aGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaa                                                            <  2:2393109/149‑1 (MQ=255)
                                                                                           gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGTTT‑TTAAAAAATAAATTAAATATTTTTTTTTTAATAAATATATAAAAAAAAAAAAAAACACAAAATAAAAATTAATAGATTGGTGAAAGGaaaaaa                                                           >  1:91770/1‑149 (MQ=255)
                                                                                              tCTGCTTTGTCTATACCACGTAATTTTTAATATCCTGTCTTTTTTTTTCTATT‑TCAAAATATATTTAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                        <  2:1516048/149‑1 (MQ=255)
                                                                                              tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATT‑TCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATAAAAAAAAAGAAAAAAATAAAAATATTTGGTGGGTGGAAGTCaataaaaa                                                        >  2:3105588/1‑148 (MQ=255)
                                                                                                     tGTCTATACCACTTAATTTTTAATATCTTGTCTTTTTTTTTCTATT‑TAAAATTATAATGAATTTTTTTGTTTTTAATTATCGATATAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTcc                                                 <  1:2141464/149‑1 (MQ=255)
                                                                                                         gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATTTTATTAAATTTTTTTTTTTTAAATTTAGAGATTACGCTGACCAAAAAGACAAAATTAAAATTATTGGGGGGGTGTAATGCAAAAAAAAGGCCTCCCGag                                             >  2:2048851/1‑148 (MQ=255)
                                                                                                         gATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TAAAAATATAATTTAATTATTTTTTTTTAAATATCGAGATAAAGATCACAAAAAAGACAATATTTAAATTATTAGAGGGGTGTAAGGCAAAAAAAATGCCTACCGAt                                             >  2:2047715/1‑149 (MQ=255)
                                                                                                           tACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATT‑TTAAAATATAATGTAATTATTTGTTTTTATATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTGGAGGTGTGAAATGCAAAATAAAGGCCTCCCGATAg                                           >  2:1374343/1‑149 (MQ=255)
                                                                                                            aCCACGTACATTTTCATATCCTGTCGTGTGTTTTAGTTT‑TGAAAAAATAATGTAATTATTTGTTTTTTAATATAGAGATAACGATCACAAAAAAGACAATATGTAAATTATTGTTGGAGTGTAAGGCAAAAAAAAGGCCACCCGCTAgg                                          >  2:1333023/1‑149 (MQ=255)
                                                                                                             ccACGTACATTTTACTATCCTGTCGTTTTTTTTCTATT‑TCAAAATATAATTAATTTTTTTGTTTTTAATTATCGAGATACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAggg                                         <  1:305655/149‑1 (MQ=255)
                                                                                                                 gtaatttttAATATCCTGTTTTTTGTTTTCTATT‑AAAAAATATACTTAATTTTTTTTTTTTTAAATATCGATATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                     <  1:2772474/144‑1 (MQ=255)
                                                                                                                 gTACATTTTCATTTCCTGTCGTTTGTTTTCTATT‑TCAAAATATAATGAAATTATTTTTTTTTAAATATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGc                                     <  2:3685825/149‑1 (MQ=255)
                                                                                                                      ttttCATATCCTGTGGTTTGTTTTCTATT‑AAAAAATATAATTAAATTTTTTTTTTTTAAATATCGTTATAACGACAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                                <  1:2678315/149‑1 (MQ=255)
                                                                                                                         tCATATCCTTTCTTTTTTTTTCTTTT‑AAAAAATATAATTAAATTTTTTTTTTTTAAATATCGATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaa                             <  1:3956222/149‑1 (MQ=255)
                                                                                                                          aatatCCTGTCGTTTTTTTTCTTTT‑TAAAATTATAATTAAATTATTTTTTTTTAAATATCGAGATACCGACCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                            <  1:2325720/148‑1 (MQ=255)
                                                                                                                           atttCCTGTGTTTTTTTTTCTTTT‑AAAAATTATAATTAATTTTTTTTTTTTTAATTTTCGATAAACCCATAAAAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAg                           <  1:1480794/146‑1 (MQ=255)
                                                                                                                            aacccTGTCTTTTGTTTTCTATT‑ACAAAATATAATGAAATTTTTTTTTTTTAAATATCGAGATAACTATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGt                          <  2:3073358/146‑1 (MQ=255)
                                                                                                                              tCCTGTCGTTTGTTTTCTATT‑TCAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgc                        <  1:3208833/149‑1 (MQ=255)
                                                                                                                                cTGTCGTTTTTTTTAGATT‑TCAAAAAATAATGAAAATATTTTTTTTTAAATATAGAGATAAAGATAACAAAAAAAAAAATATTAAAAATATTAGAGGAGTGAAATGCAAAAAAAAAGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                      >  2:2517590/1‑149 (MQ=255)
                                                                                                                                    cttttgtttTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                  <  2:2521199/147‑1 (MQ=255)
                                                                                                                                    cGTTTTTTTTCTATT‑ACAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                  <  1:2461687/149‑1 (MQ=255)
                                                                                                                                     tttttttttCTATT‑ACAAAATATAATGAAATTTTTTTTTTTTAAATATCTATATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                 <  1:1322290/148‑1 (MQ=255)
                                                                                                                                        tgtttTCGATT‑TCAAAATATAATGAAATTTTTTGTTTTTAAATATCTAGATAACTAAAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt              <  1:3105867/149‑1 (MQ=255)
                                                                                                                                        tgtttTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAACGGGCGCGTGttttt              >  2:3947147/1‑149 (MQ=255)
                                                                                                                                         gtttTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa             >  1:414599/1‑149 (MQ=255)
                                                                                                                                           tttCGATT‑TCAAATTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg           <  2:137591/149‑1 (MQ=255)
                                                                                                                                           tttCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg           >  1:2374873/1‑149 (MQ=255)
                                                                                                                                             tCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc         >  2:3934663/1‑149 (MQ=255)
                                                                                                                                               gATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATTACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg       >  2:701331/1‑149 (MQ=255)
                                                                                                                                                 tt‑tAAAAATTTAATTAAATTATTTGTTTTTAAATATCGTTATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt     <  2:2105615/149‑1 (MQ=255)
                                                                                                                                                  t‑tCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAATAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg    >  2:2922330/1‑149 (MQ=255)
                                                                                                                                                   tacAAAATATAATTAATTTTTTTTTTTTTAAATATCTATATAACGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg    <  2:415503/147‑1 (MQ=255)
                                                                                                                                                     cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg  >  2:3384852/1‑149 (MQ=255)
                                                                                                                                                     cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCg  >  1:1682364/1‑149 (MQ=255)
                                                                                                                                                     cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGGGTTTTTATGCCGGATGCg  >  2:1934054/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATT‑TCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCG  >  CP009273/2170565‑2170861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A