Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,713 C→A 30.7% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7130CA30.7% 260.1 / 31.8 183intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (37/77);  new base A (35/23);  total (83/100)
Fisher's exact test for biased strand distribution p-value = 5.85e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 185 total aligned reads displayed.

GCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGC  >  CP009273/2170568‑2170860
                                                                                                                                                 |                                                                                                                                                     
gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaa                                                                                                                                                     <  1:2783849/148‑1 (MQ=255)
gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGATTTaaa                                                                                                                                                     >  2:307997/1‑148 (MQ=255)
 cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaa                                                                                                                                                    <  2:1871766/148‑1 (MQ=255)
   gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAat                                                                                                                                                  <  2:1427233/148‑1 (MQ=255)
   gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAat                                                                                                                                                  <  2:389478/148‑1 (MQ=255)
    cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                                 <  2:3300130/148‑1 (MQ=255)
    cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAata                                                                                                                                                 >  2:1481262/1‑148 (MQ=255)
      gTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATTACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                               <  2:1207559/148‑1 (MQ=255)
       tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                                                                              <  1:3542155/148‑1 (MQ=255)
       tttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAAAATaa                                                                                                                                              >  1:2910691/1‑148 (MQ=255)
        ttCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAATATAAt                                                                                                                                             >  2:3495195/1‑148 (MQ=255)
           tACCACCACTTTCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                          <  2:3525025/148‑1 (MQ=255)
           tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                          <  1:1784059/148‑1 (MQ=255)
           tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                          <  2:2573394/148‑1 (MQ=255)
            accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                         <  2:631741/148‑1 (MQ=255)
            accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                         <  1:1236820/148‑1 (MQ=255)
             ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAATGAAat                                                                                                                                        >  2:2356483/1‑148 (MQ=255)
             ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAat                                                                                                                                        >  2:1726339/1‑148 (MQ=255)
              caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                       <  1:2894131/148‑1 (MQ=255)
              caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGTATTAAAAAaataatttaaat                                                                                                                                       >  1:2249124/1‑142 (MQ=255)
                ccacTTGCATCGGTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGAATTCAAAATATAATGTAaatat                                                                                                                                     >  1:2924628/1‑148 (MQ=255)
                 cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    <  2:2427779/148‑1 (MQ=255)
                  acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAtt‑t                                                                                                                                  <  1:2685893/148‑1 (MQ=255)
                  acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAAAATAAATAAATTAtt‑t                                                                                                                                  >  1:556378/1‑148 (MQ=255)
                      gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAAAATAATTAAAATATt‑ttttt                                                                                                                              >  2:743128/1‑148 (MQ=255)
                        aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGttttt                                                                                                                            <  1:2369088/148‑1 (MQ=255)
                        aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGttttt                                                                                                                            <  2:1196187/148‑1 (MQ=255)
                        aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAAAGAAATTATt‑ttttttt                                                                                                                            >  2:3461005/1‑148 (MQ=255)
                         tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTGATTTAAAAATATAAATAAAATATt‑tttttttt                                                                                                                           >  2:35037/1‑147 (MQ=255)
                          cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAATAAATTAAT‑TTTTTTTaa                                                                                                                          >  2:3303013/1‑148 (MQ=255)
                          cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAAAAAAAATAAAAAATt‑tttttttta                                                                                                                          >  2:1520525/1‑146 (MQ=255)
                           gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTaaa                                                                                                                         <  1:2070398/148‑1 (MQ=255)
                             ttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAata                                                                                                                       <  2:1013671/148‑1 (MQ=255)
                                  gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCgag                                                                                                                  <  2:260927/148‑1 (MQ=255)
                                  gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTCGATTTCAAAATATAAAGAAAATATT‑TTTTTTTAAATATAgat                                                                                                                  >  2:2328414/1‑147 (MQ=255)
                                  gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATT‑TTTTTTTAAATATagag                                                                                                                  >  2:536489/1‑148 (MQ=255)
                                  gaTTGTGAATATTGAATGCCGGAACCACATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCATGTCGTTTTTTTTATATTTAAAAAAAAAAACAAAATATTATTTTTTTaaaaaaaag                                                                                                                   >  1:2015292/1‑142 (MQ=255)
                                    ttGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGAt                                                                                                                <  2:983745/148‑1 (MQ=255)
                                    ttGTGAAGATTGACTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGAt                                                                                                                <  2:2137549/148‑1 (MQ=255)
                                     tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATa                                                                                                               <  1:175536/148‑1 (MQ=255)
                                        gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATT‑TGTTTTTAAATATAGAGATaaag                                                                                                            >  2:3398176/1‑146 (MQ=255)
                                           tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATc                                                                                                         <  1:3077360/148‑1 (MQ=255)
                                           tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATc                                                                                                         <  1:3077711/148‑1 (MQ=255)
                                            atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATca                                                                                                        <  1:2306281/148‑1 (MQ=255)
                                              tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGGTTTAAAAATATAATGAAATAATT‑TTTTTTTAAATATAGAGATAACGCTcaca                                                                                                      >  2:1250023/1‑148 (MQ=255)
                                                 aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                                   <  1:2440238/148‑1 (MQ=255)
                                                  tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTATTTTTTAAAATATAAATAAAATATT‑TTTTTTTTAATATATAGATAAAAATAACaaaaa                                                                                                  >  1:425360/1‑148 (MQ=255)
                                                       gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTTAAAATATAATTTAAATATT‑TTTTTTTTATTATCGAGTTAGCGTTTAAAAAAACCAct                                                                                             >  1:790478/1‑147 (MQ=255)
                                                             cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTGATTTAAAAATATAAATTAAATATT‑TTTTTTTTTATATAGAGTTAAAGATCAAAAAAAAGaaaaaaata                                                                                       >  1:1642836/1‑143 (MQ=255)
                                                              aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTGAAAAAATAATGTAAATATT‑TTTTTTTTAATATAGCGATAAAGATCACAAAAACCACAatattac                                                                                      >  1:1098991/1‑145 (MQ=255)
                                                                aaCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATT‑TGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGaaaa                                                                                    <  2:1444437/148‑1 (MQ=255)
                                                                   ccccgcGCGGGGCGTTGTACACCATCTGCTTTGACGACACCACGTACATTTTCAAAGCCGTTCGTTTGTTTTCGATTTCAAAATAAAATGAATTTATT‑TTTTTTTAAATATCGACAAAACGATCACAAACACGACAATATGAAAatta                                                                                 <  2:2710994/146‑1 (MQ=255)
                                                                       gcgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGGGTTTTGGATTTTAAAATAAAATTAAATTATT‑TTTTTTTTTATATATATATAAAGATAACAAAAAACAAAATATAaaaaaaattag                                                                             >  1:1592120/1‑141 (MQ=255)
                                                                        cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                            <  2:2222026/148‑1 (MQ=255)
                                                                        cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGAAACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                            <  2:3343521/148‑1 (MQ=255)
                                                                        cgcTGTGCGTTGTTCAGCATCGGCTTTGTCGATACCCCTTAAATTTGCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCAAAAAAACGACAATATGAAAATTATTCga                                                                            <  1:576858/148‑1 (MQ=255)
                                                                         gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTTTT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                           <  2:3493348/148‑1 (MQ=255)
                                                                          cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTGATTTAAAAATATAAATTAATTTTT‑TTTTTTTAAATATACTGATAAGGATCACAAAAAATACAATATTAAAaatattttagg                                                                          >  1:2443427/1‑143 (MQ=255)
                                                                          cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCgagg                                                                          <  2:3186300/148‑1 (MQ=255)
                                                                            gtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCgaggag                                                                        <  2:1629142/148‑1 (MQ=255)
                                                                               cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAATAATTAAATTATT‑TTTTTTTTAAAAAAGATATAAACATAAAAAAAAACACAAAAATAAAAATATTAGAGGAGTGa                                                                     >  2:1623090/1‑148 (MQ=255)
                                                                                  tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAAAATAAATAAAATATT‑TTTTTTTAAATATAGAGATAAACATAACAAAAAAGACAATAAGAAAATTATTAGAGGAGTGAAAg                                                                  >  2:412810/1‑148 (MQ=255)
                                                                                        gaaTCTGATTTGTCGATACCACGTACATTTTAATATCCTGTGTTTTTTTTTCTTTTTCAAAATATAATTAAATTTTT‑TTTTTTTAAATATCGAGATAACGAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑aaaa                                                           <  1:1765019/146‑1 (MQ=255)
                                                                                        gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAATTAAATTATT‑TTTTTTTAAATATAGAGATAAAGATAAAAAAAAAAAAAAAATTAAAAATATTAGAGGAGTGAAAGGC‑aaaa                                                           >  2:2753846/1‑148 (MQ=255)
                                                                                        gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTGAAAATATAATGAAAATAAT‑TTTTTTTAAATATCGAGTTAAACATCACAAAAAAGTAAAAATTAAAATTATTAGAGGAGGGTAAGGC‑aaat                                                           >  2:2753849/1‑147 (MQ=255)
                                                                                        gCATCGGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑aaaa                                                           <  2:2266720/148‑1 (MQ=255)
                                                                                         cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTGATTTGAAAAAATAATTAAAATATT‑TTTTTTTTTATATAGAGATAAACATCACAAAAAACACAAAATTAAAAATATTAGAGGCGGGGAAGGG‑caaaa                                                          >  2:2464345/1‑148 (MQ=255)
                                                                                         cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAATATAATGAAAATATT‑TTTTTTTAAAAATAGAGATAAACATCACAAAAAACAAAAAATGTAAATTATTAGAGGAGTGGAAGGC‑caaaa                                                          >  2:1140998/1‑148 (MQ=255)
                                                                                          aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTGGTTTTTTTTCGATTTCAAAATATAATGAAATTATT‑TTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑aaaaaa                                                         <  2:1020816/148‑1 (MQ=255)
                                                                                            cTGCTTTGTCGATACCACTAAAATTTTCATATCCTGTCTTTTGTTTTCTATTTAAAAATATAATGAATTTATT‑TTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAAc                                                       <  1:1461521/148‑1 (MQ=255)
                                                                                               cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTTATTTAAAAAAAAAAATAAAATAAT‑TTTTTTTATAAATAGAGATTACCATCACAAAAAACAAAAAATATAAAATATTAGCGGAGGGGAAGTC‑CAAAAAActtc                                                    >  2:3289721/1‑145 (MQ=255)
                                                                                                 ttGTCGATACCACGTAAATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCt                                                  <  2:1917814/148‑1 (MQ=255)
                                                                                                  tGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTc                                                 <  2:2932144/148‑1 (MQ=255)
                                                                                                    tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATT‑TTTTTTTTAATATCGAGATAACGATCACAAAAAAGACCATATTAAAATTATTAGAGGAGTGAAAGGC‑AAAAAAAAGGCCTccc                                               >  2:789243/1‑148 (MQ=255)
                                                                                                    tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATT‑TTTTTTTTAATATAGAGATAAAGATCACAAAAAAGACAATATTAAAATTATTAGAGGAGTGTAAGGC‑AAAAAAAAGGCCAccc                                               >  2:2631487/1‑148 (MQ=255)
                                                                                                     cGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTGAAAATATAATGAAAATATT‑TTTTTTTAAATATAGTGAAAAAGATCACAAAAAACAAAAAATTAAAATTATTGGTGGGGGGTAAGGC‑CAAAAAAAGGcccaccc                                              >  2:1627974/1‑143 (MQ=255)
                                                                                                      gATACCACTAAATTTTTAATATCCTGTGTTTTTTTTTCTATTACAAAATATAATCAATTTTTT‑TTTTTTTAAATATCGAGATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGa                                             <  1:2525427/148‑1 (MQ=255)
                                                                                                       aTACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCTAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGAt                                            <  2:52548/148‑1 (MQ=255)
                                                                                                        tACCACGTACATTTTCATATCCTGTCGTGTGGTTTGGATTTAAAAATATAATGTAATTTTT‑TTTTTTTTAATATAGAGATAACGATCACAAAAACGACAAAATTAAAAATATTGGAGGTGTGGAAGGG‑AAAAAAAAGGCCTcccccga                                           >  2:3058398/1‑144 (MQ=255)
                                                                                                            aggtaatttttCTTATCGTGTCTTTTTTTTTCTATTTCAAATTATATTGAAATTATT‑TTTTTTTAAATATCGAGATAACTAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGa                                       <  2:371651/141‑1 (MQ=255)
                                                                                                               taatttttCATATCCTTTCTTTTTTTTTCTATTTAAAAATATAATTAAATTATT‑TTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGc                                    <  1:875025/144‑1 (MQ=255)
                                                                                                                aaaTTTTCATATCCTGTCTTTTTTTTTCTATTTAAAAATATAATTAAATTTTT‑TGTTTTTAAATATCGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGcc                                   <  1:582719/146‑1 (MQ=255)
                                                                                                                 ctttttCATATCGTGTGTTTTTTTTTATTTTAAAAAATATAATTAATTTTTT‑TTTTTTTAATTATCGACATAACGAAAACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCg                                  <  1:3461005/146‑1 (MQ=255)
                                                                                                                  ttttaCATTTCCTGTATTTTTTTTTCTATTTAAAAATATATTTAATTTATT‑TTTTTTTAAATTTCGATATAACGAAAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGt                                 <  1:3398176/147‑1 (MQ=255)
                                                                                                                       cATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaa                            <  2:589880/148‑1 (MQ=255)
                                                                                                                       cATATCCTGTCGTGTTTTTTGGATTTCAAAATATAAAGAAATTATT‑TTTTTTTAAATATCGAGATAAACATAACAAAAAACAAAAAATGAAAATTATTAGAGGGGTGTAAGGC‑AAAAAAAACGCCTCCCCATAGGGAAGCCCTAgaca                            >  2:1088518/1‑145 (MQ=255)
                                                                                                                               gTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGcgcg                    >  2:242326/1‑148 (MQ=255)
                                                                                                                                 cGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                  >  2:186809/1‑148 (MQ=255)
                                                                                                                                  gtttttttTCTATTTAAAAATATAATGAAATTATT‑TTTTTTTAAATATCGATATAACTATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtg                  <  1:2328414/148‑1 (MQ=255)
                                                                                                                                  gtttgtttTCGATTACAAAATATAATGAAATTATT‑TTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgtgt                 <  2:219084/148‑1 (MQ=255)
                                                                                                                                   tttgtttTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                >  1:2762890/1‑148 (MQ=255)
                                                                                                                                   tttgtttTCGATTTAAAAATATATTTAAATTATT‑TGTTTTTAAATTTCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                <  2:1285810/148‑1 (MQ=255)
                                                                                                                                     tgtttTCTATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt              <  2:1906253/148‑1 (MQ=255)
                                                                                                                                         ttCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg          >  1:3136376/1‑148 (MQ=255)
                                                                                                                                             aTTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg      >  1:798161/1‑148 (MQ=255)
                                                                                                                                              tttCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa     >  1:2388753/1‑148 (MQ=255)
                                                                                                                                              tttCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa     >  1:2840264/1‑148 (MQ=255)
                                                                                                                                              tttCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa     >  1:1060851/1‑148 (MQ=255)
                                                                                                                                               ttCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt    >  2:3365800/1‑148 (MQ=255)
                                                                                                                                                 cAAAATATAATAAATTTTTT‑TTTTTTTAAATATCGATAAACCTAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc  <  1:743128/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
GCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATT‑TGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGC  >  CP009273/2170568‑2170860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A