Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,170,713 C→A 21.1% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,170,7130CA21.1% 228.9 / 5.4 114intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (26/54);  new base A (20/7);  total (53/61)
Fisher's exact test for biased strand distribution p-value = 2.47e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGC  >  CP009273/2170565‑2170860
                                                                                                                                                    |                                                                                                                                                       
ttGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCa                                                                                                                                                        <  1:4177878/150‑1 (MQ=255)
 tGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTGTTGTATTTaaa                                                                                                                                                       >  1:1244818/1‑150 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTTATTTAaaaaa                                                                                                                                                    >  1:633875/1‑149 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAat                                                                                                                                                    <  1:4320030/150‑1 (MQ=255)
    cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGTTTTCAAAat                                                                                                                                                    >  1:633777/1‑150 (MQ=255)
     aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                                   <  1:724721/150‑1 (MQ=255)
     aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                                   <  2:755511/150‑1 (MQ=255)
       cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtat‑a                                                                                                                                                <  2:3713627/150‑1 (MQ=255)
        ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑aa                                                                                                                                               <  1:3718689/150‑1 (MQ=255)
        ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑aa                                                                                                                                               <  1:3718690/150‑1 (MQ=255)
         gTTTCTACCACCACTTGCATCGGTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAt                                                                                                                                              <  2:4414903/150‑1 (MQ=255)
            tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑Gaa                                                                                                                                          <  2:1970251/150‑1 (MQ=255)
             cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTGTTTAGATTTCAAAATAT‑aaataaa                                                                                                                                          >  2:747324/1‑145 (MQ=255)
             cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAAAAT‑AAT‑Gaaa                                                                                                                                         >  2:3124616/1‑150 (MQ=255)
                  accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGGTTTAAAAATAA‑AAT‑TAAattatt                                                                                                                                    >  1:1090930/1‑150 (MQ=255)
                   acacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTAttt                                                                                                                                   <  1:491055/149‑1 (MQ=255)
                    cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATAT‑AAT‑GAAAATAtttt                                                                                                                                  >  2:1545274/1‑149 (MQ=255)
                     acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTTTTTTTAAAAAAAA‑AAT‑TAAAATAttttt                                                                                                                                 >  1:2123853/1‑148 (MQ=255)
                     acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAAAAT‑AAT‑TAAATTAttttt                                                                                                                                 >  1:3562270/1‑148 (MQ=255)
                     acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGAACATTTTCATATCCTGTCGTGTGTTTTAGATTTCAAAAAAA‑AAA‑TAAAATAttttt                                                                                                                                 >  1:3562281/1‑148 (MQ=255)
                        tGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGtttt                                                                                                                              <  2:4463034/150‑1 (MQ=255)
                          cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAA‑AAT‑TAAATTAAttttttttt                                                                                                                            >  2:292015/1‑149 (MQ=255)
                           aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTaa                                                                                                                           >  2:2634303/1‑150 (MQ=255)
                              gTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAata                                                                                                                        <  2:2230287/150‑1 (MQ=255)
                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAatat                                                                                                                       <  1:2844384/150‑1 (MQ=255)
                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAatat                                                                                                                       <  1:620950/150‑1 (MQ=255)
                               tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAatat                                                                                                                       <  1:1887114/150‑1 (MQ=255)
                                  cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCga                                                                                                                    <  2:3189806/150‑1 (MQ=255)
                                    agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAAT‑AAT‑GAAATTATTTGTTTTTAAATATCgaga                                                                                                                  >  2:358376/1‑150 (MQ=255)
                                     gaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATAT‑AAT‑TAAATTATTTGTTTTTTAatatatatat                                                                                                                 >  1:4046514/1‑144 (MQ=255)
                                      aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATa                                                                                                                <  1:2403137/150‑1 (MQ=255)
                                      aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATa                                                                                                                <  1:2403108/150‑1 (MQ=255)
                                      aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTGTTTGATTTTTAAAAATAAATATAAAATATTTTTTTTtatatagaaatt                                                                                                                  >  1:2433680/1‑144 (MQ=255)
                                         gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                             <  2:2785886/150‑1 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                           <  2:3959338/150‑1 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAAT‑AAT‑GAAATTATTTTTTTTTAAATATAGAGATAACGAt                                                                                                           >  2:1549737/1‑150 (MQ=255)
                                           gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATAT‑AAA‑GTAATTATTTGTTTTTAAATATAGAGATAAAGAt                                                                                                           >  2:2221263/1‑150 (MQ=255)
                                             atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTTGTTTTGAAAATAT‑AAT‑TGAATTATTTGTTTTTTTATATAGAGATAAAGAtaa                                                                                                         >  2:931090/1‑148 (MQ=255)
                                             atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                         <  1:3733324/150‑1 (MQ=255)
                                             atatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATca                                                                                                         <  1:1287918/150‑1 (MQ=255)
                                                ttGAATGCCGGAACCGCATAACCGCCGCGCGGTGCGGTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAATAT‑AAA‑GAAATTATTTGTTTTTAAATATCGCGATAACCATCACaa                                                                                                      >  2:2355151/1‑150 (MQ=255)
                                                  gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTAAAAAAAT‑AAA‑GAAATTATTTGTTTTTTAATAAAGAGATAACGATCaaaaaa                                                                                                    >  2:92484/1‑150 (MQ=255)
                                                  gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTTTTTTTAAATATAGAGATAACGATCACaaaa                                                                                                    >  2:2521939/1‑150 (MQ=255)
                                                   aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                   <  2:4340816/150‑1 (MQ=255)
                                                    aTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAAc                                                                                                  <  1:4216316/150‑1 (MQ=255)
                                                     tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACg                                                                                                 <  1:1163110/150‑1 (MQ=255)
                                                     tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACg                                                                                                 <  1:1163127/150‑1 (MQ=255)
                                                     tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACg                                                                                                 <  1:1163828/150‑1 (MQ=255)
                                                     tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACg                                                                                                 <  1:912793/150‑1 (MQ=255)
                                                      gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTTAAAAAAA‑AAT‑TTAAATTTTTTTTTTTTAATATATTGATAAAGATaaaaaaaaaca                                                                                                >  1:2289231/1‑147 (MQ=255)
                                                      gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTAAAAATAT‑AAT‑TTAATTATTTGTTTTTTTATATAGATATAACAATaaaaaaaaaaa                                                                                                >  1:2289194/1‑147 (MQ=255)
                                                       ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc                                                                                               <  1:3960572/150‑1 (MQ=255)
                                                       ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAT‑AAT‑GAAATTATTTTTTTTTAAATATAGAGATAAAGATCACAAAAAAGAc                                                                                               >  2:747405/1‑150 (MQ=255)
                                                                cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAA‑GAAATTATTTGTTTTTTAATATAGAGATAACGATAACAAaaaagaaaaaaagaaa                                                                                      >  2:3081464/1‑150 (MQ=255)
                                                                 aTAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATAT‑AAT‑TTAATTATTTTTTTTTTTAAATCGAGATAACGATCAAAAAAAAGACAatattatca                                                                                     >  2:3052906/1‑145 (MQ=255)
                                                                  tAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTCAAAATAT‑AAT‑GAAATTATTTTTTTTTAAATATCGTGATAACGATCACAAAAACGACAATATGAAAat                                                                                    <  2:1569744/150‑1 (MQ=255)
                                                                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATAT‑AAT‑TAAATTATTTTTTTTTAAATATAGAGATAACGATCAAAAAAAAGACAAAATGAAAattatta                                                                               >  2:3621279/1‑149 (MQ=255)
                                                                        ccgcGCTGTGCGTTGTACAGCACCTGCTTTGTCGATCCCACGTACATTTTCAAAACCTGTCGTTTGTTTTCGATTACAAAAAAT‑AAT‑CAAATTATTTTTTTTTAAATATCTATAAAACGATCACAAAAACGACAATATGAAAATTATTCg                                                                              <  2:1008196/150‑1 (MQ=255)
                                                                           cgcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAATAT‑AAT‑GAAATTATTTGTTTTTTAATATCGAGATAACGATAACAAAAAAGACAAAATTAAAAATATTAgagg                                                                           >  2:1819889/1‑150 (MQ=255)
                                                                             cTGTGGGTTGTTCAGCATCTGCTTTGTCGAACCCACTTACATTTACAAATCCTGTCGTTTTTTTTCTATTAAAAAATAT‑AAT‑CAATTTATTTTTTTTTAAATATCTAGATAACGACCACAAACACGACAATATGAAAATTATTCgaggag                                                                         <  1:964353/150‑1 (MQ=255)
                                                                              tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAATAT‑AAA‑GAAATTATTTGTTTTTAAATATAGAGATAACGATAACAAAAAACACAAAATTAAAATTATTAGAGgtgt                                                                        >  2:1853524/1‑150 (MQ=255)
                                                                               ttgggttgtgCAGCATCTGCTTTTTCGATACAACGTACATTTTCATATCCTGTCGTTTTTTTTATTTTTCAAAATAT‑AAT‑TCAATTTTTTGTTTTTAAATATCTTGATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTg                                                                       <  2:3091331/146‑1 (MQ=255)
                                                                               gtgCGTTGTTCAGCATCTGCTTTGTCGATCCCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTACAAAATAT‑AAT‑CAAATTATTTTTTTTTAAATATCGCTATAACTAACACAAACACGACAATATGAAAATTATTCGAGGAGTg                                                                       <  1:2326414/150‑1 (MQ=255)
                                                                                    ttgttCAGCATCTGCTTTGTCTATACCACTTACATTTACATATCCTGTCGTTTTTTTTCTATTACAAAATAT‑ATT‑TAATTTATTTTTTTTTAAATATCGAGATAACTAACACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                  <  1:1167120/150‑1 (MQ=255)
                                                                                    ttgtaCAGCATCTGCTTTGTCTATACCACTAACATTTTCATATCCTGTGGTTTGTTTTCTATTACAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAgg                                                                  <  1:1167065/150‑1 (MQ=255)
                                                                                     tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGTTTTGAAAAAAT‑AAT‑TTAATTATTTTTTTTTTAAAATAGTGATTAAGATAACAAAAAAGACAAAATGAAAAATATTAGAGGAGtttaatgg                                                                 >  1:3107284/1‑143 (MQ=255)
                                                                                        tCAGCATGTGCTTTGTCGATACCACGAAATTTTTAATATCCGGTCTTTTTTTTTCTATTTAAAAATAT‑AAT‑TAAATTTTTTTTTTTTAAATATCTATATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑aaa                                                            <  1:2205607/150‑1 (MQ=255)
                                                                                         cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATAA‑AAT‑GAAAATATTTGTTTTTAAATATAGATATAAAGATCAAAAAAAAGACAAAATTAAAAATATTAGAGGGGTGAAAGGC‑‑aaaa                                                           >  2:2139865/1‑150 (MQ=255)
                                                                                            cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTTGTTTTAAAAAAATAAAT‑AAAATAATTTTTTTTTAAA‑ATAGAGAAAAAGATAAAAAAAAAAACAAAAATAAAAAAATTATAGGAGTGTAATTG‑‑aaaaaaa                                                        >  1:2125116/1‑150 (MQ=255)
                                                                                              tgtGCTTTGTCTATACCACGTAAATTTTCATATCCTGTCGTTTTTTTTCTATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACg                                                      <  2:2564844/148‑1 (MQ=255)
                                                                                               cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCTTTTGTTTTCGATTTAAAAATAT‑AAT‑GAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACgg                                                     <  2:3149686/150‑1 (MQ=255)
                                                                                                tgatttGTCTATACCACTTACATTTTCATATCATTTCTTTTGTTTTCTATTAAAAAATAT‑AAT‑TAAATTATTTGTTTTTAAATATCGATATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGc                                                    <  2:1083184/147‑1 (MQ=255)
                                                                                                      gTCGATACCACGTACTTTTTCATATCCTGTCTTTTGTTTTCTATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCg                                              <  2:1121703/150‑1 (MQ=255)
                                                                                                      gTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑TAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCg                                              <  2:1121739/150‑1 (MQ=255)
                                                                                                       tCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATAA‑AAT‑GAAATTATTTTTTTTTAAATATAGAGATAACGATAAAAAAAAAGACAAAATGAAAATTATTAGAGGAGTGAAAGGC‑‑AAAAAAAAGGCCTCCCGa                                             >  2:2717329/1‑150 (MQ=255)
                                                                                                            aCCACGTACATTTTCATATCCTGTCGTGTTTTTTCGTTTTAAAAATAT‑AAT‑GAAATTATTTTTTTTTAAATATAGAGTTAACGATAACAAAAAAGAAAATATGAAAATTATTAGAGGTGTGAAAGGC‑‑ACAAAAAAGGCCTCCCGCTAggg                                        >  2:2784512/1‑150 (MQ=255)
                                                                                                               acttACTTTTTCATAGCCTGTCTTTTGTTTTCTATTTCAAAATAT‑AAT‑GAAATTATTTTTTTTTAAATATCGATATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAg                                     <  1:700624/147‑1 (MQ=255)
                                                                                                                cGTACATTTTCATTTCATGTCGTTTTTTTTCGATTTAAAAATAT‑AAT‑GAAATTTTTTTTTTTTAAATATCTAGAAAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGc                                    <  2:4154047/150‑1 (MQ=255)
                                                                                                                   aCATTTTCATATCCTGTCGTGTGTTTTAGGTTTAAAAAAAT‑AAA‑TAAAATATTTTTTTTTTAATATAGATATAAAGAAAACAAAAAAAAAAAAATTAAAATTAATCGATGGGGGAAATTC‑‑AAAAAAAAAGCCACCCGAAAGGGTAgacct                                 >  2:2912458/1‑146 (MQ=255)
                                                                                                                    cATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATAA‑AAA‑GAAAATAATTGTTTTTTAATATCGAGATAAAGATAACAAAAAACAAAAAATGAAAAATATTAGAGGAGTGGAAGGC‑‑CAAAAAAACGCCACCCCCTAAGGCAGCCgaa                                >  2:4670986/1‑148 (MQ=255)
                                                                                                                     tttttCATATCCTGTCTTTTTTTTTCTATTTCAAATTAT‑AAT‑GAAATTATTTTTTTTTAAATATCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                               <  2:166990/149‑1 (MQ=255)
                                                                                                                     aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                               <  2:166792/150‑1 (MQ=255)
                                                                                                                     aTTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAg                               <  2:4418954/150‑1 (MQ=255)
                                                                                                                                      tttgtttTCTATTTAAAAATAT‑AAT‑TAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtttt              <  1:1545274/150‑1 (MQ=255)
                                                                                                                                        ttttttCTATTTCAAAATAT‑AAT‑TAATTTTTTTTTTTTTAAATATCGATATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa            <  1:292015/148‑1 (MQ=255)
                                                                                                                                        ttttttCTATTACAAAATAT‑AAT‑TAAATTATTTGTTTTTAAATATCGAGATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa            <  2:1497114/148‑1 (MQ=255)
                                                                                                                                        tgtttTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa            >  2:3479505/1‑150 (MQ=255)
                                                                                                                                          ttttCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa            >  2:3314608/1‑150 (MQ=255)
                                                                                                                                          ttttCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCA‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt           >  2:3314736/1‑150 (MQ=255)
                                                                                                                                          ttttCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCA‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGGGTTTTTAt           >  2:3314540/1‑150 (MQ=255)
                                                                                                                                             tCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg       <  2:2816374/150‑1 (MQ=255)
                                                                                                                                             tCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg       >  2:3638115/1‑150 (MQ=255)
                                                                                                                                             tCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGAGAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg       >  2:3637939/1‑150 (MQ=255)
                                                                                                                                               gATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa     >  1:1838545/1‑150 (MQ=255)
                                                                                                                                               gATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa     >  1:1838173/1‑150 (MQ=255)
                                                                                                                                               gATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCAGGa     >  1:1840619/1‑150 (MQ=255)
                                                                                                                                                aTTTCAAAATAT‑AAT‑GAATTTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt    <  1:2755094/150‑1 (MQ=255)
                                                                                                                                                 ttacAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATg   <  2:328135/147‑1 (MQ=255)
                                                                                                                                                  ttCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc  >  1:4029072/1‑150 (MQ=255)
                                                                                                                                                  ttCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc  >  1:4029111/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
TTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAT‑AAT‑GAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGC‑‑AAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGC  >  CP009273/2170565‑2170860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A